Abstract
Taurine is thought to affect bone in rats favorably. However, studies on the actions of this estrogen deficiency and high cholesterol diet factors on the bone metabolism are limited. In this study, the protective effect of taurine on bone was determined. Thirty-two 42 days old female SD rats were placed in individual stainless cages. Given to rats was fed to chow (Samyang Corporation, South Korea) and deionized water for a 4 days adaptation period. After the period of adaptation, Half of the rats were induced estrogen deficiency model by ovariectomy (OVX), and the left rats with sham-operated were used control (SHAM). For six weeks, the OVX and SHAM rats had separately a 2% taurine supplemented diet with ad libitum in both the water and the food. DEXA for small animals (PIXImus, GE Lunar co, Wisconsin) was used to determine spinal and femoral bone. The concentrations of serum calcium and phosphorus were also measured. The monitoring of bone formation was done by determining the serum ALP and osteocalcin. Urinary DPD the values were determined as index of bone resorption. Statistical measure was done with SAS (version 9.3). A lower overall intake of the daily food was observed in non-ovariectomized rats than in the OVX rats. At sacrifice, a much greater body weight was observed in ovariectomized group compare to non-operated group. That difference was absent in both fed taurine SHAM and OVX rats. Serum calcium and phosphorus were not statistically different by taurine supplementation. Urinary excretion of calcium was not effected by taurine supplementation. Serum ALP and was significantly decreased by taurine in OVX rats (p < 0.05). For the spine BMD and BMC, there was no difference among SHAM and OVX rats by taurine. Spine BMC per body weight of taurine groups were higher than control groups (p < 0.1). No significant difference was observed after taurine supplementation in femur BMD and BMC. The analysis of the results suggest that taurine supplementation modulates the bone mineral contents in postmenopausal model rats fed with high cholesterol diet.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- ALP:
-
Alkaline phosphatase
- BMC:
-
Bone mineral contents
- BMD:
-
Bone mineral density
- DPD:
-
Deoxypyridinoline
- OVX:
-
Ovariectomized
- SHAM:
-
Sham operated
References
Bleibler F, Konnopka A, Benzinger P, Rapp K, Konig HH (2013) The health burden and costs of incident fractures attributable to osteoporosis from 2010 to 2050 in Germany—a demographic simulation model. Osteoporos Int 24:835–847
Bonjour JP, Benoit V, Rousseau B, Souberbielle JC (2012) Consumption of vitamin D-and calcium-fortified soft white cheese lowers the biochemical marker of bone resorption TRAP 5b in postmenopausal women at moderate risk of osteoporosis fracture. J Nutr 142:698–703
Choi MJ (2009) Effects of taurine supplementation on bone mineral density in ovariectomized rats fed calcium deficient diet. Nutr Res Pract 3:108–113
Choi MJ, DiMarco NM (2009) The effects of dietary taurine supplementation on bone mineral density in ovariectomized rats. Adv Exp Med Biol 643:341–349
Choi MJ, Seo JN (2013) Effect of taurine feeding on bone mineral density and bone markers in rats. Adv Exp Med Biol 776:51–58
Colaianni G, Brunetti G, Faienza MF, Colucci S, Grano M (2014) Osteoporosis and obesity: role of Wnt pathway in human and murine models. World J Orthop 5:242–246
Crepaldi G, Romanato G, Tonin P, Maggi S (2007) Osteoporosis and body composition. J Endocrinol Investig 30:42–47
Dong XL, Li CM, Cao SS, Zhou LP, Wong MS (2016) A high-saturated-fat, high-sucrose diet aggravates bone loss in ovariectomized female rats. J Nutr 146:1172–1179
Grimble RF (2006) The effects of sulfur amino acid intake on immune function in humans. J Nutr 136:1660S–1665S
Guo HY, Jiang L, Ibrahim SA, Zhang L, Zhang H, Zhang M, Ren FZ (2009) Orally administered lactoferrin preserves bone mass and microarchitecture in ovariectomized rats. J Nutr 139:958–964
Hill Gallant KM, Weaver CM, Towler DA, Thuppal SV, Bailey RL (2016) Nutrition in cardioskeletal health. Adv Nutr 7:544–555
Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:175–192
Kim KC, Shin DH, Lee SY, Im JA, Lee DC (2010) Relation between obesity and bone mineral density and vertebral fractures in Korean postmenopausal women. Yonsei Med J 51:857–863
Ko KS, Backus RC, Berg JR, Lame MW, Rogers QR (2007) Differences in taurine synthesis rate among dogs relate to differences in their maintenance energy requirement. J Nutr 137:1171–1175
Ko KS, Tôrres CL, Fascetti AJ, Stipanuk MH, Hirschberger L, Rogers QR (2006) Copper deficiency does not lead to taurine deficiency in rats. J Nutr 136:2502–2505
Kontny E, Szczepanska K, Kowalczewski J, Kurowska M, Janicka I, Marcinkiewicz J, Maslinski W (2000) The mechanism of taurine chloramine inhibition of cytokine (interleukin-6, interleukin-8) production by rheumatoid arthritis fibroblast-like synoviocytes. Arthritis Rheum 43:2169–2177
Lee HR, Hong SS, Lee SY, Cho YH, Park HJ, Jung DW, Lee JG, Kim YJ, Kim JE (2011) The impact of body weight change on bone mineral density of the lumbar spine in perimenopausal women: a retrospective, one-year follow-up study. Korean J Fam Med 32:219–225
Longo AB, Ward WE (2016) PUFAs, Bone mineral density, and fragility fracture: findings from human studies. Adv Nutr 7:299–312
McElroy JF, Wade GN (1987) Short-and long-term effects of ovariectomy on food intake, body weight, carcass composition, and brown adipose tissue in rats. Physiol Behav 39:361–365
Moon PD, Kim MH, Lim HS, Oh HA, Nam SY, Han NR, Kim MJ, Jeong HJ, Kim HM (2015) Taurine, a major amino acid of oyster, enhances linear bone growth in a mouse model of protein malnutrition. Biofactors 41:190–197
Murakami S, Kondo Y, Sakurai T, Kitajima H, Nagate T (2002) Taurine suppresses development of atherosclerosis in Watanabe heritable hyperlipidemic (WHHL) rabbits. Atherosclerosis 163:79–87
Nieves JW (2005) Osteoporosis: the role of micronutrients. Am J Clin Nutr 81:1232S–1239S
Rauch F, Middelmann B, Cagnoli M, Keller KM, Schönau E (1997) Comparison of total alkaline phosphatase and three assays for bone-specific alkaline phosphatase in childhood and adolescence. Acta Paediatr 86:583–587
Rizzoli R (2014) Dairy products, yogurts, and bone health. Am J Clin Nutr 99:1256S–1262S
Shen CL, Chyu MC, Wang JS (2013) Tea and bone health: steps forward in translational nutrition. Am J Clin Nutr 98:1694S–1699S
Ueki I, Stipanuk MH (2009) 3T3-L1 Adipocytes and rat adipose tissue have a high capacity for taurine synthesis by the cysteine dioxygenase/cysteinesulfinate decarboxylase and cysteamine dioxygenase pathways. J Nutr 139:207–214
Yokogoshi H, Mochizuki H, Nanami K, Hida Y, Miyachi F, Oda H (1999) Dietary taurine enhances cholesterol degradation and reduces serum and liver cholesterol concentrations in rats fed a high-cholesterol diet. J Nutr 129:1705–1712
You L, Sheng ZY, Tang CL, Chen L, Pan L, Chen JY (2011) High cholesterol diet increases osteoporosis risk via inhibiting bone formation in rats. Acta Pharmacol Sin 32:1498–1504
Yuan LQ, Liu W, Cui RR, Wang D, Meng JC, Xie H, Wu XP, Zhou HD, Lu Y, Liao EY (2010) Taurine inhibits osteoclastogenesis through the taurine transporter. Amino Acids 39:89–99
Zhang Y, Lai WP, Leung PC, Wu CF, Wong MS (2007) Short- to mid-term effects of ovariectomy on bone turnover, bone mass and bone strength in rats. Biol Pharm Bull 30:898–903
Zhao LJ, Jiang H, Papasian CJ, Maulik D, Drees B, Hamilton J, Deng HW (2008) Correlation of obesity and osteoporosis: effect of fat mass on the determination of osteoporosis. J Bone Miner Res 23:17–29
Acknowledgement
Dong-A Pharmaceutical Co donated taurine, Maeil dairies Co. donated casein.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media B.V.
About this paper
Cite this paper
Choi, MJ. (2017). Taurine May Modulate Bone in Cholesterol Fed Estrogen Deficiency-Induced Rats. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_87
Download citation
DOI: https://doi.org/10.1007/978-94-024-1079-2_87
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-024-1077-8
Online ISBN: 978-94-024-1079-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)