Abstract
Polybrominated diphenyl ethers (PBDEs) are globally dispersed throughout the environment, and the levels of some PBDEs in the environment may still be increasing. Previous studies showed that BDE 209 exerted neurodevelopmental and neurobehavioral effects in humans and animals. Oxidative stress is a common mechanism reported in PBDEs-induced neurotoxicity. Taurine, as an antioxidant, whether it is effective in alleviating BDE 209-induced neurotoxicity is still unknown. PC12 cells were exposed to various concentrations of BDE 209 (6.25, 12.5, 25, 50, and 100 μM). 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used to assess the cell viability. 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) detector was used to explore the production of ROS. Acridine orange was used to reflect the permeation of lysosomal membrane. Rhodamine 123 was used to reflect the permeation of mitochondrial membrane. Lactate dehydrogenase and catalase in PC12 cells exposed to BDE 209 were examined by kits. The results showed that taurine could significantly reverse the decreased viability, the serious oxidative stress and abnormal autophagy in PC12 cells exposed to BDE 209. Collectively, our results indicated that taurine could protect PC12 cells from BDE 209-induced neurotoxicity by alleviating oxidative stress.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- CAT:
-
Catalase
- LDH:
-
Lactate dehydrogenase
- PBDEs:
-
Polybrominated diphenyl ethers
- ROS:
-
Reactive oxygen species
References
Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
Bodas M, Van Westphal C, Carpenter-Thompson R, Mohanty DK, Vij N (2016) Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment. Free Radic Biol Med 97:441–453
Chen H, Tang X, Zhou B, Xu N, Wang Y (2016) Mechanism of Deca-BDE-induced apoptosis in Neuro-2a cells: role of death-receptor pathway and reactive oxygen species-mediated mitochondrial pathway. J Environ Sci (China) 46:241–251
Costa LG, Giordano G (2011) Is decabromodiphenyl ether (BDE-209) a developmental neurotoxicant? Neurotoxicology 32:9–24
Frederiksen M, Vorkamp K, Thomsen M, Knudsen LE (2009) Human internal and external exposure to PBDEs—a review of levels and sources. Int J Hyg Environ Health 212:109–134
La Guardia M J, Hale R C, Harvey E (2006) Detailed polybrominated diphenyl ether (PBDE) congener composition of the widely used penta-, octa-, and deca-PBDE technical flame-retardant mixtures[J]. Environmental science and technology 40(20):6247–6254
Liang SX, Gao HX, Zhao YY, Ma XM, Sun HW (2010) Effects of repeated exposure to decabrominated diphenyl ether (BDE-209) on mice nervous system and its self repair. Environ Toxicol Pharmacol 29:297–301
Liu X, Wang J, Lu C, Zhu C, Qian B, Li Z, Liu C, Shao J, Yan J (2015) The role of lysosomes in BDE 47-mediated activation of mitochondrial apoptotic pathway in HepG2 cells. Chemosphere 124:10–21
Rahman F, Langford KH, Scrimshaw MD, Lester JN (2001) Polybrominated diphenyl ether (PBDE) flame retardants. Sci Total Environ 275:1–17
Sarkar D, Chowdhury JP, Singh SK (2016) Effect of polybrominated diphenyl ether (BDE-209) on testicular steroidogenesis and spermatogenesis through altered thyroid status in adult mice. Gen Comp Endocrinol 239:50–61. doi:10.1016/j.ygcen.2015.11.009
Sayed RH, Salem HA, El-Sayeh BM (2012) Potential protective effect of taurine against dibromoacetonitrile-induced neurotoxicity in rats. Environ Toxicol Pharmacol 34:849–857
Shaki F, Hosseini MJ, Ghazi-Khansari M, Pourahmad J (2012) Toxicity of depleted uranium on isolated rat kidney mitochondria. Biochim Biophys Acta 1820:1940–1950
Suzuki N, Miller G, Morales J, Shulaev V, Torres MA, Mittler R (2011) Respiratory burst oxidases: the engines of ROS signaling. Curr Opin Plant Biol 14:691–699
Tagliaferri S, Caglieri A, Goldoni M, Pinelli S, Alinovi R, Poli D, Pellacani C, Giordano G, Mutti A, Costa LG (2010) Low concentrations of the brominated flame retardants BDE-47 and BDE-99 induce synergistic oxidative stress-mediated neurotoxicity in human neuroblastoma cells. Toxicol In Vitro 24:116–122
Tang MZ, Wang ZF, Shi YL (2004) Involvement of cytochrome c release and caspase activation in toosendanin-induced PC12 cell apoptosis. Toxicology 201:31–38
Zhang C, Liu F, Liu X, Chen D (2010) Protective effect of N-acetylcysteine against BDE-209-induced neurotoxicity in primary cultured neonatal rat hippocampal neurons in vitro. Int J Dev Neurosci 28:521–528
Acknowledgements
This work was supported by the National Natural Science Foundation of China (81302400/H2601 to X.L.; 81273031/H2601 to J.S.); and the startup funding from Dalian Medical University under Talent Introduction Program (201069 to J.S.).
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media B.V.
About this paper
Cite this paper
Liu, Q. et al. (2017). Role of Taurine in BDE 209-Induced Oxidative Stress in PC12 Cells. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_71
Download citation
DOI: https://doi.org/10.1007/978-94-024-1079-2_71
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-024-1077-8
Online ISBN: 978-94-024-1079-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)