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Taurine 10 pp 403-411 | Cite as

Increased N-Acetyltaurine in the Skeletal Muscle After Endurance Exercise in Rat

  • Teruo Miyazaki
  • Yuho Nakamura
  • Kei Ebina
  • Takaki Mizushima
  • Song-Gyu Ra
  • Keisuke Ishikura
  • Yasushi Matsuzaki
  • Hajime Ohmori
  • Akira Honda
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 975)

Abstract

Taurine is metabolized to a novel metabolite, N-acetyltaurine (NAT), through N-acetylation with acetate. Furthermore, NAT production increases when the endogenous production of acetate is elevated in some situations, such as alcohol catabolism and endurance exercise. We have previously reported that both the serum concentration and urinary excretion of NAT from humans were increased after endurance exercise, and that NAT was secreted by cultured skeletal muscle cells exposed to both acetate and taurine. The present study evaluated the hypothesis that NAT is synthesized in the skeletal muscle after endurance exercise. Normal rats were loaded to a transient treadmill running until exhaustion. Serum, skeletal muscle, and liver were collected immediately after the exercise. The NAT concentration in the plasma and in the soleus muscle from the exercised rats was significantly increased compared to that in the samples from the sedentary control rats. There was a significant positive correlation in the NAT concentration between the plasma and soleus muscle. The NAT concentration in the liver was unchanged after the endurance exercise. These results confirm that the significantly increased NAT in both the serum and urine after endurance exercise is derived from NAT synthesis in the skeletal muscle.

Keywords

Treadmill running N-Acetylation Acetate Acetyl-CoA Energy production 

Abbreviations

ACS2

Acetyl-CoA synthetase 2

EX group

Exercise group

IS

Internal standard

NAT

N-acetyltaurine

PDH

Pyruvate dehydrogenase

SED group

Sedentary group

TCA

Trichloroacetic acid

Notes

Acknowledgments

This study was supported in part by Kakenhi grants (25750334 (Miyazaki 2013–2015)) from the Japan Society for the Promotion of Science.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Teruo Miyazaki
    • 1
  • Yuho Nakamura
    • 2
  • Kei Ebina
    • 3
  • Takaki Mizushima
    • 2
  • Song-Gyu Ra
    • 4
  • Keisuke Ishikura
    • 5
  • Yasushi Matsuzaki
    • 1
  • Hajime Ohmori
    • 2
  • Akira Honda
    • 1
  1. 1.Tokyo Medical University Ibaraki Medical CenterIbarakiJapan
  2. 2.University of TsukubaIbarakiJapan
  3. 3.Seitoku UniversityChibaJapan
  4. 4.Fukuoka UniversityFukuokaJapan
  5. 5.Sojo UniversityKumamotoJapan

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