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Taurine 10 pp 319–328Cite as

Taurine Increases Insulin Expression in STZ-Treated Rat Islet Cells In Vitro

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 975))

Abstract

This research aims at figure out the effects and the pathway of taurine on insulin in islet cells cultured in vitro treated by STZ. In the experiment, islet cells were isolated from pancreatic tissue by in situ perfusion with collagenase V. The pancreatic islet cells, maintained in RPMI 1640 culture medium were divided into six groups: C: control, E: supplemented with 10 mmol/L of taurine, group M, T1, T2 and T3 was treated with STZ (0.5 mmol/L), at the same time, taurine were added in group T1,T2 and T3 for 30 min, and then culture medium were collected by centrifugation and then insulin levels were detected by radioimmunoassay, the cells were then rinsed with Hanks, and 0,10, 0, 5, 10, 20 mmol/L of taurine in group C, E, M, T1, T2 and T3 were added for 24 h respectively. Total RNA was extracted, then insulin gene and its transcription regulator such as PDX-1, NeuroD1 were amplified by semi-quantitative RT-PCR. The results showed that, the release of insulin from islet cells treated by STZ could be inhibited by taurine, gene expression of insulin, PDX-1 and NeuroD1 in STZ group decreased significantly, which were up-regulated by taurine administration. In conclusion, taurine exerts a certain degree of protective and reconstructive effects on islet cells treated by STZ.

$Shumei Lin and Gaofeng Wu are Co-first authors

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Abbreviations

NeuroD1:

Norvegicus neurogenic differentiation 1

PDX-1:

Pancreatic and duodenal homeobox 1

STZ:

Streptozotocin

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Acknowledgements

This study was supported by a grant from the National Natural Science Foundation of China (No. 31402160; 31572481; 31302051 and 31502026), doctoral initiating project of Liaoning province (No. 20111083) and Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province (No. 2014049).

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    Authors and Affiliations

    1. Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, 110866, People’s Republic of China

      Shumei Lin, Gaofeng Wu, Dongdong Zhao, Jie Han, Qunhui Yang, Ying Feng, Mei Liu, Jiancheng Yang & Jianmin Hu

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    1. Shumei Lin
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    2. Gaofeng Wu
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    3. Dongdong Zhao
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    Correspondence to Jiancheng Yang or Jianmin Hu .

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    Editors and Affiliations

    1. Department of Life Sciences, University of Seoul, Seoul, South Korea

      Dong-Hee Lee  & Ha Won Kim  & 

    2. College of Medicine, University of South Alabama, Mobile, Alabama, USA

      Stephen W. Schaffer

    3. Department of Developmental Neurobiology, NY State Institute for Basic Research in Developmental Disabilities, New York, USA

      Eunkyue Park

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    Lin, S. et al. (2017). Taurine Increases Insulin Expression in STZ-Treated Rat Islet Cells In Vitro. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_28

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