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High-Pressure Fluorescence Spectroscopy

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High Pressure Bioscience

Part of the book series: Subcellular Biochemistry ((SCBI,volume 72))

Abstract

The combination of fluorescence and pressure perturbation is a widely used technique to study the effect of pressure on a protein system to obtain thermodynamic, structural and kinetic information on proteins. However, we often encounter the situation where the available pressure range up to 400 MPa of most commercial high-pressure fluorescence spectrometers is insufficient for studying highly pressure-stable proteins like inhibitors and allergenic proteins. To overcome the difficulty, we have recently developed a new high-pressure fluorescence system that allows fluorescence measurements up to 700 MPa. Here we describe the basic design of the apparatus and its application to study structural and thermodynamic properties of a couple of highly stable allergenic proteins, hen lysozyme and ovomucoid, using Tryptophan and Tyrosine/Tyrosinate fluorescence, respectively. Finally, we discuss the utility and the limitation of Trp and Tyr fluorescence. We discuss pitfalls of fluorescence technique and importance of simultaneous use of other high-pressure spectroscopy, particularly high-pressure NMR spectroscopy.

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Author notes

  1. Kazuyuki Akasaka

    Present address: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, 606-8522, Japan

Authors and Affiliations

  1. High Pressure Protein Research Center, Institute of Advanced Technology, Kinki University, 930 Nishimitani, Kinokawa, 649-6493, Japan

    Akihiro Maeno & Kazuyuki Akasaka

Authors
  1. Akihiro Maeno
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  2. Kazuyuki Akasaka
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Correspondence to Akihiro Maeno .

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Editors and Affiliations

  1. Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Kyoto, Japan

    Kazuyuki Akasaka

  2. Department of Life System, Institute of Technology and Science, Tokushima University, Tokushima, Japan

    Hitoshi Matsuki

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Maeno, A., Akasaka, K. (2015). High-Pressure Fluorescence Spectroscopy. In: Akasaka, K., Matsuki, H. (eds) High Pressure Bioscience. Subcellular Biochemistry, vol 72. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9918-8_32

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