Skip to main content

Cultivation and Breeding of Cannabis sativa L. for Preparation of Standardized Extracts for Medicinal Purposes

  • Chapter

Part of the book series: Medicinal and Aromatic Plants of the World ((MAPW,volume 1))

Abstract

Cannabis sativa L. (marijuana; Cannabaceae) is a plant with worldwide distribution, yielding fiber and food, as well as a psychoactive drug. Cannabinoids and in particular the main psychoactive Δ9-THC are promising substances for the development of new drugs and are of high therapeutic potential. This review gives an overview of Cannabis classification, the current verities, botanical features, genomics, chemicals constituents, cellular site and biosynthesis of cannabinoids. Furthermore, the different cultivation and breeding forms, changes in cannabinoids over time, method of harvesting, drying and processing of Cannabis are extensively described in addition to the analytical procedures for standardization of Cannabis based medicinal extracts. Finally, some aspects of current approved Cannabis based medicine and its ways of administration are described.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Appendino G, Chianese G, Taglialatela-Scafati O (2011) Cannabinoids: occurrence and medicinal chemistry. Curr Med Chem 18(7):1085–1099

    Article  CAS  PubMed  Google Scholar 

  • Argo WR, Fischer PR (2002) Understanding pH management for container-grown crops. Meister, Willoughby

    Google Scholar 

  • Barnicomparini I, Ferri S, Centini F (1984) Cannabinoid level in the leaves as a tool for the early discrimination of Cannabis chemovariants. Forensic Sci Int 24(1):37–42

    Article  CAS  Google Scholar 

  • Barrett ML, Scutt AM, Evans FJ (1986) Cannflavin-A and Cannflavin-B, prenylated flavones from Cannabis sativa L. Experientia 42(4):452–453

    Article  CAS  PubMed  Google Scholar 

  • Beresford TP (1993) Marijuana the forbidden medicine. Grinspoon, L, Bakalar, Jb. Psychosomatics 34(4):368–369

    Article  Google Scholar 

  • Bohlmann F, Hoffmann E (1979) Compounds from Helichrysum umbraculigerum related to cannabigerol. Phytochemistry 18(8):1371–1374

    Article  CAS  Google Scholar 

  • Brenneisen R (1983) Psychotropic drugs. I. Cannabis sativa L. (Cannabinaceae). Pharm Acta Helv 58(11):314–320

    CAS  PubMed  Google Scholar 

  • Brenneisen R (2007) Chemistry and analysis of phytocannabinoids and other Cannabis constituents. In: Elsohly MA (ed) Marijuana and the cannabinoids. Humana Press, Totowa, pp 17–49

    Chapter  Google Scholar 

  • Brischia R, Piccioni E, Standardi A (2002) Micropropagation and synthetic seed in M.26 apple rootstock (II): a new protocol for production of encapsulated differentiating propagules. Plant Cell Tiss Org 68(2):137–141

    Article  Google Scholar 

  • Brunner TE (1973) Marijuana in Ancient Greece and Rome? The literary evidence. Bull Hist Med 47(4):344–355

    Google Scholar 

  • Cervantes J (2006) Marijuana horticulture: The indoor/outdoor medical grower’s bible. Van Patten, Vancouver, p 41

    Google Scholar 

  • Chandra SH, Lata I, Khan A, ElSohly MA (2010) Propagation of elite Cannabis sativa for the production of Δ9-Tetrahydrocannabinol (THC) using biotechnological tools. In: Rajesh A (ed) Medicinal plant biotechnology. CABI, Cambridge, MA, pp 98–114

    Google Scholar 

  • Choi YH, Hazekamp A, Peltenburg-Looman AMG, Frederich M, Erkelens C, Lefeber AWM, Verpoorte R (2004) NMR assignments of the major cannabinoids and cannabiflavonoids isolated from flowers of Cannabis sativa. Phytochem Anal 15(6):345–354

    Article  CAS  PubMed  Google Scholar 

  • Clarke RC, Watson DP (2002) Botany of natural Cannabis medicines. In: Grotenhermen F, Russo E (eds) Cannabis and Cannabinoids: pharmacology, toxicology, and therapeutic potential. The Haworth Press, New York, pp 3–13

    Google Scholar 

  • Coene T (1995) Greenhouse coverings uncovered. The Growing Edge 6(3):66–72

    Google Scholar 

  • De Backer B, Debrus B, Lebrun P, Theunis L, Dubois N, Decock L, Verstraete A, Hubert P, Charlier C (2009) Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material. J Chromatogr B 877(32):4115–4124

    Article  CAS  Google Scholar 

  • De Backer B, Maebe K, Verstraete AG, Charlier C (2012) Evolution of the content of THC and other major cannabinoids in drug-type Cannabis cuttings and seedlings during growth of plants. J Forensic Sci 57(4):918–922

    Article  PubMed  CAS  Google Scholar 

  • de Meijer EP, Bagatta M, Carboni A, Crucitti P, Moliterni VM, Ranalli P, Mandolino G (2003) The inheritance of chemical phenotype in Cannabis sativa L. Genetics 163(1):335–346

    PubMed Central  PubMed  Google Scholar 

  • Demeijer EPM, Vansoest LJM (1992) The CPRO Cannabis germplasm collection. Euphytica 62(3):201–211

    Article  Google Scholar 

  • Dioscorides P (1968) The Greek herbal of Dioscorides, AD 1655. Hafner, London

    Google Scholar 

  • Ebbell B (1937) The papyrus Ebers, the greatest Egyptian medical document. Levin & Munksgaard/H. Milford/ Oxford University Press, Copenhagen/London

    Google Scholar 

  • Ebers G (1875) Das hermetische buch über die arzneimittel der alten Ägypten in heratischer schrift. 2Bde., Leipzing

    Google Scholar 

  • Elbatsh MM, Moklas MA, Marsden CA, Kendall DA (2012) Antidepressant-like effects of delta(9)-tetrahydrocannabinol and rimonabant in the olfactory bulbectomised rat model of depression. Pharmacol Biochem Behav 102(2):357–365

    Article  CAS  PubMed  Google Scholar 

  • ElSohly MA, Slade D (2005) Chemical constituents of marijuana: the complex mixture of natural cannabinoids. Life Sci 78(5):539–548

    Article  CAS  PubMed  Google Scholar 

  • ElSohly MA, Turner CE, Phoebe CH, Knapp JE, Schiff PL, Slatkin DJ (1978) Anhydrocannabisativine, a new alkaloid from Cannabis sativa. J Pharm Sci 67:124

    Article  CAS  PubMed  Google Scholar 

  • Fairbairn JW, Rowan MG (1975) Cannabinoid pattern in Cannabis sativa L seedlings as an indication of chemical race. J Pharmacy Pharmacol 27:P90–P90

    Article  Google Scholar 

  • Fankhauser M (2002) History of cannabis in Western Medicine. In: Cannabis and Cannabinoids. The Haworth Integrative Healing Press, New York

    Google Scholar 

  • Feeney M, Punja ZK (2003) Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.). In Vitro Cell Dev-Pl 39(6):578–585

    Article  CAS  Google Scholar 

  • Fellermeier M, Eisenreich W, Bacher A, Zenk MH (2001) Biosynthesis of cannabinoids – incorporation experiments with C-13-labeled glucoses. Eur J Biochem 268(6):1596–1604

    Article  CAS  PubMed  Google Scholar 

  • Ferioli V, Rustichelli C, Pavesi G, Gamberini G (2000) Analytical characterisation of Hashish samples. Chromatographia 52(1–2):39–44

    Article  CAS  Google Scholar 

  • Fetterma P, Keith ES, Waller CW, Guerrero O, Doorenbo NJ, Quimby MW (1971) Mississippi-grown Cannabis sativa L- preliminary observation on chemical definition of phenotype and variations in tetrahydrocannabinol content versus age, sex, and plant part. J Pharm Sci 60(8):1246–1249

    Article  Google Scholar 

  • Fischedick JT, Hazekamp A, Erkelens T, Choi YH, Verpoorte R (2010) Metabolic fingerprinting of Cannabis sativa L, cannabinoids and terpenoids for chemotaxonomic and drug standardization purposes. Phytochemistry 71(17–18):2058–2073

    Article  CAS  PubMed  Google Scholar 

  • Fisse J, Braut F, Cosson L, Paris M (1981) Étude in vitro des capacités organogénétiques de tissus de Cannabis sativa L.; Effet de différentes substances de croissance. Plantes Médicinales et Phytotherapie 15:217–223

    CAS  Google Scholar 

  • Flemming T, Muntendam R, Steup C, Kayser O (2007) Chemistry and biological activity of tetrahydrocannabinol and its derivatives. In: Khan MTH (ed) Topics in heterocyclic chemistry: bioactive heterocycles IV, vol 10. Springer, Berlin, pp 1–42

    Chapter  Google Scholar 

  • Formukong EA, Evans AT, Evans FJ (1989) The medicinal uses of Cannabis and its constituents. Phytother Res 3(6):219–231

    Article  CAS  Google Scholar 

  • Fox P, Bain PG, Glickman S, Carroll C, Zajicek J (2004) The effect of Cannabis on tremor in patients with multiple sclerosis. Neurology 62(7):1105–1109

    Article  CAS  PubMed  Google Scholar 

  • Gaoni Y, Mechoula R (1966) Structure of cannabichromene a new active constituent of Hashish. J Chem Soc Chem Commun 1:20–21

    Google Scholar 

  • Gaoni Y, Mechoulam R (1964) Isolation, structure, and partial synthesis of an active constituent of Hashish. J Am Chem Soc 86(8):1646–1647

    Article  CAS  Google Scholar 

  • Grotenhermen F, Russo E (2002) Chemical constituents of Cannabis: pharmacology, toxicology, and therapeutic potential. In: ElSohly MA (ed) Cannabis and Cannabinoids. Haworth Press, New York, pp 27–36

    Google Scholar 

  • Guy GW, Stott C (2005) The development of Sativex®- a natural cannabis based medicine. In: Mechoulam R, Bruinrels J (eds) Cannabinoids as therapeutics milestones in drug therapy. Springer, Berlin, pp 231–263

    Chapter  Google Scholar 

  • Hao YJ, Deng XX (2003) Genetically stable regeneration of apple plants from slow growth. Plant Cell Tiss Org 72(3):253–260

    Article  CAS  Google Scholar 

  • Happyana N, Agnolet S, Muntendam R, Van Dam A, Schneider B, Kayser O (2013) Analysis of cannabinoids in laser-microdissected trichomes of medicinal Cannabis sativa using LCMS and cryogenic NMR. Phytochemistry 87:51–59

    Article  CAS  PubMed  Google Scholar 

  • Hazekamp A (2006) An evaluation of the quality of medicinal grade cannabis in the Netherlands. Cannabinoids 1(1):1–9

    Google Scholar 

  • Hazekamp A, Justin TF, Díez ML, Lubbe A, Ruhaak RL (2010) 3.24 – Chemistry of Cannabis. Comprehensive Natural Products II 3:1033–1084

    Google Scholar 

  • Heinz KM, Nelson JM (1996) Interspecific interactions among natural enemies of Bemisia in an inundative biological control program. Biol Control 6(3):384–393

    Article  Google Scholar 

  • Hendriks H, Malingre TM, Batterman S, Bos R (1975) Monoterpene and sesquiterpene hydrocarbons of essential oil of Cannabis sativa. Phytochemistry 14(3):814–815

    Article  CAS  Google Scholar 

  • Hillig KW, Mahlberg PG (2004a) A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaceae). Am J Bot 91(6):966–975

    Article  CAS  PubMed  Google Scholar 

  • Hillig KW, Mahlberg PG (2004b) A chemotaxonomic analysis of cannabinoid variation in Cannabis (Cannabaceae). Am J Bot 91(6):966–975

    Article  CAS  PubMed  Google Scholar 

  • Holdcroft A, Maze M, Dore C, Tebbs S, Thompson S (2006) A multicenter dose-escalation study of the analgesic and adverse effects of an oral Cannabis extract (Cannador) for postoperative pain management. Anesthesiology 104(5):1040–1046

    Article  CAS  PubMed  Google Scholar 

  • Jones JB (1997) Hydroponics: a practical guide for the soilless grower. Lucie Press, Bocan Raton

    Google Scholar 

  • Kim ES, Mahlberg PG (1997) Immunochemical localization of tetrahydrocannabinol (THC) in cryofixed glandular trichomes of Cannabis (Cannabaceae). Am J Bot 84(3):336–342

    Article  CAS  PubMed  Google Scholar 

  • Kim ES, Mahlberg PG (2003) Secretory vesicle formation in the secretory cavity of glandular trichomes of Cannabis sativa L. (Cannabaceae). Mol Cells 15(3):387–395

    CAS  PubMed  Google Scholar 

  • Kubota C, Thomson CA (2006) Controlled environments for production of value-added food crops with high phytochemical concentrations: lycopene in tomato as an example. Hortscience 41(3):522–525

    CAS  Google Scholar 

  • Lata H, Chandra S, Khan IA, ElSohly MA (2008) Propagation of Cannabis sativa L. using synthetic seed technology. Planta Med 74(3):328–328

    Article  Google Scholar 

  • Lata H, Chandra S, Khan IA, ElSohly MA (2009) Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. an important medicinal plant. Physiol Mol Biol Plants 15(1):79–86

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Lata H, Chandra S, Khan I, ElSohly M (2010a) Cannabis sativa L. Micropropagation in temporary immersion bioreactor system. Planta Med 76 (P9)

    Google Scholar 

  • Lata H, Chandra S, Khan IA, ElSohly MA (2010b) High frequency plant regeneration from leaf derived callus of high delta(9)-tetrahydrocannabinol yielding Cannabis sativa L. Planta Med 76(14):1629–1633

    Article  CAS  PubMed  Google Scholar 

  • Lata H, Chandra S, Mehmedic Z, Khan IA, ElSohly MA (2012) In vitro germplasm conservation of high Delta(9)-tetrahydrocannabinol yielding elite clones of Cannabis sativa L. under slow growth conditions. Acta Physiol Plant 34(2):743–750

    Article  CAS  Google Scholar 

  • Latta R, Easton B (1975) Seasonal fluctuations in cannabinoid in cannabinoid content of Kansas marijuana. Econ Bot 29:153–163

    Article  CAS  Google Scholar 

  • Le Bot J, Adamowicz S, Robin P (1998) Modelling plant nutrition of horticultural crops: a review. Sci Hortic Amsterdam 74(1–2):47–82

    Article  Google Scholar 

  • Leggett T (2006) A review of the world cannabis situation. B Narcotics 58(1–2):1–155

    CAS  Google Scholar 

  • Lercker G, Bocci F, Frega N, Bortolomeazzi R (1992) Cannabinoid acids analysis. Farmaco 47(3):367–378

    CAS  PubMed  Google Scholar 

  • Li HL (1974) Archeological and historical account of Cannabis in China. Econ Bot 28(4):437–448

    Article  Google Scholar 

  • Lynn AB, Herkenham M (1994) Localization of cannabinoid receptors and nonsaturable high-density cannabinoid binding sites in peripheral tissues of the rat: implications for receptor-mediated immune modulation by cannabinoids. J Pharmacol Exp Ther 268(3):1612–1623

    CAS  PubMed  Google Scholar 

  • Mahlberg PG, Hemphill JK (1983) Effect of light quality on cannabinoid content of Cannabis sativa L (Cannabaceae). Bot Gaz 144(1):43–48

    Article  CAS  Google Scholar 

  • Mahlberg PG, Kim ES (2004) Accumulation of cannabinoids in glandular trichomes of Cannabis (Cannabaceae). J Indust Hemp 9:15–36

    Article  CAS  Google Scholar 

  • Malingre T, Hendriks H, Batterman S, Bos R, Visser J (1975) The essential oil of Cannabis sativa. Planta Med 28(1):56–61

    Article  CAS  PubMed  Google Scholar 

  • Mandolino G, Ranalli P (1999) Advances in biotechnological approaches for hemp breeding and industry. In: Ranalli P (ed) Advances in hemp research. The Haworth Press, New York, pp 185–212

    Google Scholar 

  • Mandolino G, Carboni A, Forapani S, Faeti V, Ranalli P (1999) Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.). Theor Appl Genet 98(1):86–92

    Article  CAS  Google Scholar 

  • Mandolino G, Bagatta M, Carboni A, Ranalli P, de Meijer EPM (2003) Qualitative and quantitative aspects of the inheritance of chemical phenotype in Cannabis. J Ind Hemp 8:51–72

    Article  CAS  Google Scholar 

  • Mathur J, Ahuja PS, Lal N, Mathur AK (1989) Propagation of Valeriana-Wallichii Dc using encapsulated apical and axial shoot buds. Plant Sci 60(1):111–116

    Article  Google Scholar 

  • Mcpartland JM (1983) Fungal pathogens of Cannabis sativa in central illinois. Phytopathology 73(5):797–797

    Google Scholar 

  • McPartland JM (1991) Common names for diseases of Cannabis sativa L. Plant Disease 75:226–227

    Google Scholar 

  • McPartland J, Mediavilla V (2002) Noncannabinoid components. In: Grotenhermen F, Russo E (eds) Cannabis and Cannabinoids pharmacology, toxicology, and therapeutic. Potential Haworth Press, New York, pp 401–409

    Google Scholar 

  • McPartland JM, Clarke RC, Watson DP (2000) Hemp diseases and pests: management and biological control: an advanced treatise. CABI Pub, New York

    Book  Google Scholar 

  • Mechoulam R (1988) Alkaloids in Cannabis sativa L. In: Brossi A (ed) The Alkaloids. Academic, San Diego, pp 77–93

    Google Scholar 

  • Mechoulam R, Shvo Y (1963) Hashish-I. The structure of cannabidiol. Tetrahedron Lett 19:2073–2078

    Article  CAS  Google Scholar 

  • Morimoto S, Komatsu K, Taura F, Shoyama Y (1998) Purification and characterization of cannabichromenic acid synthase from Cannabis sativa. Phytochemistry 49(6):1525–1529

    Article  CAS  PubMed  Google Scholar 

  • Muntendam R, Erkelens T, Kayser O (2009) Genetic and metabolic studies of cannabinoids in standardized medicinal Cannabis sativa. Planta Med 75(4):417–417

    Article  Google Scholar 

  • Muntendam R, Happyana N, Erkelens T, Bruining F, Kayser O (2012) Time dependent metabolomics and transcriptional analysis of cannabinoid biosynthesis in Cannabis sativa var. bedrobinol and bediol grown under standardized condition and with genetic homogeneity. Online Int J Med Plant Res 1(2):31–40

    Google Scholar 

  • Nahas GG (1982) Hashish in Islam 9th century to 18th century. B New York Acad Med 58(9):814–831

    CAS  Google Scholar 

  • Pacifico D, Miselli F, Carboni A, Moschella A, Mandolino G (2008) Time course of cannabinoid accumulation and chemotype development during the growth of Cannabis sativa L. Euphytica 160(2):231–240

    Article  CAS  Google Scholar 

  • Page JE, Nagel J (2006) Biosynthesis of terpenophenolic metabolites in Hop and Cannabis. J Recent Adv Phytochem 40:179–210

    Article  CAS  Google Scholar 

  • Paris M, Boucher F, Cosson L (1975) Constituents of Cannabis sativa Pollen. Econ Bot 29(3):245–253

    Article  CAS  Google Scholar 

  • Pattnaik S, Chand PK (2000) Morphogenic response of the alginate encapsulated axillary buds from in vitro shoot cultures of six mulberries. Plant Cell Tiss Org 60(3):177–185

    Article  Google Scholar 

  • Pec J, Flores-Sanchez IJ, Choi YH, Verpoorte R (2010) Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by (1)H-NMR spectroscopy. Biotechnol Lett 32(7):935–941

    Article  CAS  PubMed  Google Scholar 

  • Petri G, Oroszlan P, Fridvalszky L (1988) Histochemical detection of hemp trichomes and their correlation with the THC content. Acta Biol Hung 39(1):59–73

    CAS  PubMed  Google Scholar 

  • Porterfield DM, Musgrave ME (1998) The tropic response of plant root to oxygen: oxitropism in Pisum sativum L. Planta Med 206:1–6

    Article  CAS  Google Scholar 

  • Potter DJ (2004) Growth and morphology of medicinal Cannabis. In: Guy GW, Whittle BA, Robson PJ (eds) The medicinal uses of Cannabis and Cannabinoids. Pharmaceutical Press, London, pp 17–54

    Google Scholar 

  • Raharjo TJ, Verpoorte R (2004) Methods for the analysis of cannabinoids in biological materials: a review. Phytochem Analy 15(2):79–94

    Article  CAS  Google Scholar 

  • Rahn EJ, Hohmann AG (2009) Cannabinoids as pharmacotherapies for neuropathic pain: from the bench to the bedside. Neurotherapeutics 6(4):713–737

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Raman A (ed) (1998) The Cannabis plant: botany, cultivation, and processing for use. Cannabis: the Genus Cannabis. Harwood Academic Publishers, Amsterdam

    Google Scholar 

  • Razdan RK, Puttick AJ, Zitko BA, Handrick GR (1972) Hashish.6. Conversion of (−)-delta1(6)-tetrahydrocannabinol to (−)-delta1(7)-tetrahydrocannabinol – stability of (−)-delta1- and ″(−)-delta1(6)-tetrahydrocannabinols. Experientia 28(2):121–123

    Article  CAS  Google Scholar 

  • Rosenthal E (ed) (1984) Marijuana growers handbook; indoor/greenhouse. Quick American Publishing Company, San Fransisco

    Google Scholar 

  • Ross SA, ElSohly MA (1995) Constituents of Cannabis sativa L. XXVIII-A review of the natural constituents: 1980–1994. Zagazig J Pharm Sci 4:1–10

    CAS  Google Scholar 

  • Ross SA, ElSohly MA (1996) The volatile oil composition of fresh and air-dried buds of Cannabis sativa. J Nat Prod 59(1):49–51

    Article  CAS  PubMed  Google Scholar 

  • Ross SA, Mehmedic Z, Murphy TP, Elsohly MA (2000) GC-MS analysis of the total delta9-THC content of both drug and fiber type cannabis seeds. J Anal Toxicol 24(8):715–717

    Article  CAS  PubMed  Google Scholar 

  • Ross SA, ElSohly MA, Sultana GNN, Mehmedic Z, Hossain CF, Chandra S (2005) Flavonoid glycosides and cannabinoids from the pollen of Cannabis sativa L. Phytochem Anal 16(1):45–48

    Article  CAS  PubMed  Google Scholar 

  • Russo EB (2007) History of cannabis and its preparations in saga, science, and sobriquet. Chem Biodivers 4(8):1614–1648

    Article  CAS  PubMed  Google Scholar 

  • Russo EB (2011) Taming THC: potential cannabis synergy and phytocannabinoid-terpenoid entourage effects. Br J Pharmacol 163(7):1344–1364

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Russo EB, Jiang HE, Li X, Sutton A, Carboni A, del Bianco F, Mandolino G, Potter DJ, Zhao YX, Bera S, Zhang YB, Lu EG, Ferguson DK, Hueber F, Zhao LC, Liu CJ, Wang YF, Li CS (2008) Phytochemical and genetic analyses of ancient Cannabis from Central Asia. J Exp Bot 59(15):4171–4182

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Rustichelli C, Ferioli V, Vezzalini F, Gamberini G (1996) Simultaneous separation and identification of hashish constituents by coupled liquid chromatography mass spectrometry (HPLC-MS). Chromatographia 43(3–4):129–134

    Article  CAS  Google Scholar 

  • Rustichelli C, Ferioli V, Baraldi M, Zanoli P, Gamberini G (1998) Analysis of cannabinoids in fiber hemp plant varieties (Cannabis sativa L.) by high performance liquid chromatography. Chromatographia 48(3–4):215–222

    Article  CAS  Google Scholar 

  • Sicher RC, Bunce JA (1997) Relationship of photosynthetic acclimation to changes of Rubisco activity in field-grown winter wheat and barley during growth in elevated carbon dioxide. Photosynth Res 52(1):27–38

    Article  CAS  Google Scholar 

  • Sicurani M, Piccioni E, Standardi A (2001) Micropropagation and preparation of synthetic seed in M.26 apple rootstock I: attempts towards saving labor in the production of adventitious shoot tips suitable for encapsulation. Plant Cell Tiss Org 66(3):207–216

    Article  CAS  Google Scholar 

  • Slatkin DJ, Doorenbo NJ, Harris LS, Masoud AN, Quimby MW, Schiff PL (1971) Chemical constituents of Cannabis sativa L root. J Pharm Sci 60(12):1891–1892

    Article  CAS  PubMed  Google Scholar 

  • Stahl E, Kunde R (1973) Neue Inhaltsstoffe aus dem atherischen 01 von Cannabis sativa. Tetrahedron Lett 30:2841–2844

    Article  Google Scholar 

  • Stamler RT, Fahlman RC, Vigeant H (1985) Illicit traffic and abuse of cannabis in Canada. B Narcotics 37(4):37–49

    CAS  Google Scholar 

  • Staquet M, Gantt C, Machin D (1978) Effect of a nitrogen analog of tetrahydrocannabinol on cancer pain. Clin Pharmacol Ther 23(4):397–401

    Article  CAS  PubMed  Google Scholar 

  • Steiner AA (1961) A universal method for preparing nutrient solutions of certain desired composition. Plant and Soil 15(2):134–154

    Article  CAS  Google Scholar 

  • Taguchi C, Taura F, Tamada T, Shoyama Y, Shoyama Y, Tanaka H, Kuroki R, Morimoto S (2008) Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa. Acta Crystallogr F 64:217–220

    Article  CAS  Google Scholar 

  • Taura F, Morimoto S, Shoyama Y, Mechoulam R (1995) First direct evidence for the mechanism of delta(1)-tetrahydricannabinolic acid biosynthesis. J Am Chem Soc 117(38):9766–9767

    Article  CAS  Google Scholar 

  • Taura F, Morimoto S, Shoyama Y (1996) Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid. J Biol Chem 271(29):17411–17416

    Article  CAS  PubMed  Google Scholar 

  • Taura F, Sirikantaramas S, Shoyama Y, Shoyama Y, Morimoto S (2007) Phytocannabinoids in Cannabis sativa: Recent studies on biosynthetic enzymes. Chem Biodivers 4(8):1649–1663

    Article  CAS  PubMed  Google Scholar 

  • Thakur GA, Duclos RI, Makriyannis A (2005) Natural cannabinoids: templates for drug discovery. Life Sci 78(5):454–466

    Article  CAS  PubMed  Google Scholar 

  • Tius MA, Kannangara GSK (1992) Synthesis of 5′-(2h3)-(−)-11-nor-9-carboxy-delta-9-tetrahydrocannabinol methyl-ester methyl-ether. Tetrahedron 48(42):9173–9186

    Article  CAS  Google Scholar 

  • Toyota M, Shimamura T, Ishii H, Renner M, Braggins J, Asakawa Y (2002) New bibenzyl cannabinoid from the New Zealand liverwort Radula marginata. Chem Pharm Bull 50(10):1390–1392

    Article  CAS  PubMed  Google Scholar 

  • Trofin IG, Dabija G, Vaireanu DI, Filipescu L (2012) The influence of long term storage conditions on the stability of cannabinoids derived from Cannabis resin. Rev Chim-Bucharest 63(4):422–427

    CAS  Google Scholar 

  • Turner CE, Cheng PC, Lewis GS, Russell MH, Sharma GK (1979) Constituents of Cannabis sativa. 15. Botanical and chemical profile of Indian variants. Planta Med 37(3):217–225

    Article  CAS  Google Scholar 

  • Van Patten GF (1992) Hydroponics for the rest of us. Growing Edge 3(3):24–51

    Google Scholar 

  • Vanhoenacker G, Van Rompaey P, De Keukeleire D, Sandra P (2002) Chemotaxonomic features associated with flavonoids of cannabinoid-free cannabis (Cannabis sativa subsp sativa L.) in relation to hops (Humulus lupulus L.). Nat Prod Lett 16(1):57–63

    Article  CAS  PubMed  Google Scholar 

  • Vanhove W, Van Damme P, Meert N (2011) Factors determining yield and quality of illicit indoor cannabis (Cannabis spp.) production. Forensic Sci Int 212(1–3):158–163

    Article  CAS  PubMed  Google Scholar 

  • Veress T, Szanto JI, Leisztner L (1990) Determination of cannabinoid acids by high performance liquid chromatography of their neutral derivatives formed by thermal decarboxylation. 1. Study of the decarboxylation process in open reactors. J Chromatogr 520:339–347

    Article  CAS  Google Scholar 

  • Vogelmann AF, Turner JC, Mahlberg PG (1988) Cannabinoid composition in seedlings compared to adult plants of Cannabis sativa. J Nat Prod 51(6):1075–1079

    Article  CAS  Google Scholar 

  • Waldo AZ (2006) History of cannabis as a medicine: a review. Rev Bras Psiquiatr 28(2):153–157

    Article  Google Scholar 

  • Whittle BA (2007) Cannabis as a medicine (benefits and risks associated with phytomedicines). J Pharm Pharmacol 59:A69–69

    Google Scholar 

  • Whittle BA, Guy GW (2004) Development of cannabis based medicines: risk, benefit and serendipity. In: Guy GW, Robson PJ, Whittle BA (eds) The medicinal uses of Cannabis and Cannabinoids. Pharmaceutical Press, London, pp 427–463

    Google Scholar 

  • Zhang WJ, Bjorn LO (2009) The effect of ultraviolet radiation on the accumulation of medicinal compounds in plants. Fitoterapia 80(4):207–218

    Article  CAS  PubMed  Google Scholar 

  • Zheng YB, Zhang P, Dixon M (2005) Evaluation of four lamp types for the production of tomato plants in controlled environments. Horttechnology 15(3):646–652

    Google Scholar 

  • Zulfiqar F, Ross SA, Slade D, Ahmed SA, Radwan MM, Ali Z, Khan IA, ElSohly MA (2012) Cannabisol, a novel delta(9)-THC dimer possessing a unique methylene bridge, isolated from Cannabis sativa. Tetrahedron Lett 53(28):3560–3562

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Oliver Kayser .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Farag, S., Kayser, O. (2015). Cultivation and Breeding of Cannabis sativa L. for Preparation of Standardized Extracts for Medicinal Purposes. In: Máthé, Á. (eds) Medicinal and Aromatic Plants of the World. Medicinal and Aromatic Plants of the World, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9810-5_9

Download citation

Publish with us

Policies and ethics