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Endothelial Cell Tube Formation on Basement Membrane to Study Cancer Neoangiogenesis

  • Amelia Casamassimi
  • Filomena de Nigris
  • Concetta Schiano
  • Claudio Napoli

Abstract

In this chapter we will describe the use of the tubular network assay as a useful tool in the evaluation of cancer neoangiogenesis (as reported by Arnaoutova et al. [Angiogenesis 12:267–274, 2009]). Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. A detailed working protocol applicable to the study of any type of endothelial cells is provided below and in addition, details on data interpretation analysis and troubleshooting.

Keywords

Tube formation Cancer neoangiogenesis Human aortic endothelial cells Data analysis 

References

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Key Web-Addresses for Further Information

  1. Web site address to download ImageJ software. http://rsbweb.nih.gov/ij/

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Amelia Casamassimi
    • 1
  • Filomena de Nigris
    • 1
  • Concetta Schiano
    • 2
  • Claudio Napoli
    • 1
    • 2
    • 3
  1. 1.Department of Biochemistry, Biophysics and General PathologySecond University of NaplesNaplesItaly
  2. 2.Institute of Diagnostic and Nuclear Development (SDN)IRCCSNaplesItaly
  3. 3.U.O.C. Division of Immunohematology, Transfusion Medicine and Transplantation Immunology (SIMT) and Regional Reference Laboratory of Immunogenetics and Transplantation Immunology (LIT)Second University of NaplesNaplesItaly

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