Endothelial Cell Tube Formation on Basement Membrane to Study Cancer Neoangiogenesis
In this chapter we will describe the use of the tubular network assay as a useful tool in the evaluation of cancer neoangiogenesis (as reported by Arnaoutova et al. [Angiogenesis 12:267–274, 2009]). Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. A detailed working protocol applicable to the study of any type of endothelial cells is provided below and in addition, details on data interpretation analysis and troubleshooting.
KeywordsTube formation Cancer neoangiogenesis Human aortic endothelial cells Data analysis
Key Web-Addresses for Further Information
- Web site address to download ImageJ software. http://rsbweb.nih.gov/ij/