Abstract
Basic in vitro angiogenesis assays conducted on endothelial cells of various origin are critically important in allowing us to identify mechanisms responsible for modulation of vascularization during pathological events and disease. In this chapter, we review methods for and measurement of proliferation, migration and tube-like structure formation in cerebral endothelial cultures. Alternative protocols, materials required to conduct these assays, and example results are also provided. Accurate reproducibility of these assays is highly important and therefore we have provided a section on trouble shooting near to the end. The assays are applicable to endothelial cells of any origin with minor modifications and optimizations.
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Acknowledgments and Funding
Supported in part by the National Institutes of Health. Materials including figures in this chapter have been extensively drawn from our previously published papers including: Xing et al., J Neurosci Res, 2009; Hayakawa et al., J Neurochem, 2012; Hayakawa et al., PNAS, 2012.
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Hayakawa, K., Liang, A.CL., Xing, C., Lo, E.H., Arai, K. (2015). In Vitro Angiogenesis Assay: Endothelial Migration, Proliferation, and Tube Formation. In: Slevin, M., McDowell, G. (eds) Handbook of Vascular Biology Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9716-0_1
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DOI: https://doi.org/10.1007/978-94-017-9716-0_1
Publisher Name: Springer, Dordrecht
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