Advancement of Emerging Tools in Synthetic Biology for the Designing and Characterization of Genetic Circuits

  • Vijai Singh
  • Indra Mani
  • Dharmendra Kumar Chaudhary


Bioengineering of synthetic metabolic pathways is a valuable tool for production of useful products and basic understanding of the biological complexity. Thus, we require high-throughput cloning and characterization tools and technologies. Standard cloning techniques have limitation for construct size and slow process. Here, we underline recent advancement and development of high-throughput technologies which can help to accelerate the synthetic biology research. It can be useful for the construction of large gene cassettes for production of drugs, bio-fuels, therapeutics and also rewired the natural systems; and created novel gene networks. In this chapter, we confer the different gene cloning methods for assembly of gene networks and their characterization using recent synthetic biology tools. It can be useful for accelerating the synthetic biology research for human welfare.


Bioengineering Gene network Microfluidics Genetic circuits Synthetic biology 



Authors wish to thank A.K. Singh, Satya Prakash and Pritee Singh for providing the suggestions, encouragement and fruitful discussion during preparation of this chapter.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Vijai Singh
    • 1
  • Indra Mani
    • 3
    • 2
  • Dharmendra Kumar Chaudhary
    • 2
  1. 1.Synth-Bio Group, Institute of Systems & Synthetic Biology University of EvryGenopole Campus 1, Genavenir 6 5 rue Henri DesbruèresÉVRYFrance
  2. 2.National Bureau of Fish Genetic ResourcesLucknowIndia
  3. 3.Department of BiochemistryBanaras Hindu UniversityVaranasiIndia

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