An Overview of Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry and Some of Its Applications

  • Mark W. DuncanEmail author
  • David Gibson
  • Ryan Walsh
  • Afshan Masood
  • Hicham Benabdelkamel
Conference paper
Part of the NATO Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA)


Matrix-assisted laser desorption/ionization time-of-flight (TOF) mass spectrometry (MS) is a powerful tool for both the qualitative and quantitative analysis of wide array of analytes. MALDI-TOF MS is simple, both in design and practice, and offers the advantages that analysis times are very short, the mass range is virtually unlimited, and sensitivity is high. This chapter describes the theory and practice of MALDI and illustrates the utility of approach by reviewing its common applications.


MALDI Mass spectrometry Microbiology Clinical applications Imaging 



We thank Dr Marvin Vestal and his colleagues at SimulTOF Systems, Sudbury, MA, for supplying the two figures that are included in this manuscript.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Mark W. Duncan
    • 1
    Email author
  • David Gibson
    • 2
  • Ryan Walsh
    • 3
  • Afshan Masood
    • 1
  • Hicham Benabdelkamel
    • 1
  1. 1.Proteomics Resource, Obesity Research Center, College of MedicineKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Northern Ireland Centre for Stratified MedicineUniversity of UlsterLondonderryUK
  3. 3.Division of Endocrinology, Metabolism & Diabetes, Department of MedicineUniversity of Colorado Anschutz Medical CampusAuroraUSA

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