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Production of Biomass and Bioactive Compounds Using Bioreactor Technology pp 625–651Cite as

Production of Ginsenosides from Adventitious Root Cultures of Panax ginseng

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Abstract

Ginsenosides are a type of glycosylated triterpenes produced as secondary metabolites by Panax ginseng (ginseng). The ginsenosides contain many physiologically and pharmacologically active ones, which possess cardio-protective, immunomodulatory, antifatigue, hepato-protective and anti-tumor properties. Field cultivation of the ginseng plant is a traditional production system to obtain the ginseng bioactive components like ginsenosides, but it is a time-consuming and labour-intensive process. It takes 5–7 years to attain maturity and to reach the harvesting stage, during which a close attention is needed as growth is subjected to several conditions such as soil, climate, pathogens and pests. The use of cell and organ culture has been sought as a potential alternative for efficient production of secondary compounds from ginseng, and various bioprocessing techniques have been developed over the past decades. Powerful strategies for adventitious root culture of ginseng have been developed for biomass and ginsenoside accumulation in large-scale bioreactors. The present status and the art of production of ginseng adventitious roots and ginsenosides from bioreactor cultures are described in this chapter.

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Abbreviations

2, 4 – D:

2, 4-Dichlorophenoxy acetic acid

DW:

Dry weight

FW:

Fresh weight

IBA:

Indole-3-butyric acid

MJ:

Methyl jasmonate

MS:

Murashige and Skoog medium

NAA:

α-naphthalene acetic acid

NO:

Nitric oxide

PUFAs:

Polyunsaturated fatty acids

SA:

Salicylic acid

TCMGRs:

Tissue culture mountain ginseng adventitious roots

WPM:

Woody plant medium

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Acknowledgement

This study was supported by a grant of Korea Healthcare Technology R&D project, Ministry of Health and Welfare, Republic of Korea (Grant No. A103017). Dr. H. N. Murthy is thankful to Ministry of Education, Science and Technology, Republic of Korea for the award of Brain pool Fellowship (131S-4-3-0523) and this paper was studied with the support of Ministry of Science, ICT and Planning (MSIP). JJZ acknowledges the support from National Natural Science Foundation of China (No. 21176153) and the Program of Shanghai Subject Chief Scientist (No. 14XD1402600).

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Authors and Affiliations

  1. Research Centre for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Hosakatte Niranjana Murthy & Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, 580003, India

    Hosakatte Niranjana Murthy

  3. Natural Resources Research Institute, R&D Headquarters, Korea Ginseng Corporation, 22, Gajeong-ro, Shinseong-dong, Yusenong-gu, 305-805, Republic of Korea

    Yun-Soo Kim

  4. Osong Medical Innovation Foundation, Clinical Drug Manufacturing Center, Chungbuk, 363-951, Republic of Korea

    Cheol-Seung Jeong

  5. Gwacheon National Museum, Ministry of Science, ICT and Future Planning, 110 Sanghabeol-ro, Gwacheon-Si, Gyeonggi-do, 427-060, Republic of Korea

    Sun-Ja Kim

  6. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai, 200240, China

    Jian-Jiang Zhong

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  1. Hosakatte Niranjana Murthy
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Editors and Affiliations

  1. Department of Horticultural Science, Chungbuk National University, Cheongju, Korea, Republic of (South Korea)

    Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, India

    Hosakatte Niranjana Murthy

  3. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

    Jian-Jiang Zhong

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Murthy, H.N., Kim, YS., Jeong, CS., Kim, SJ., Zhong, JJ., Paek, KY. (2014). Production of Ginsenosides from Adventitious Root Cultures of Panax ginseng . In: Paek, KY., Murthy, H., Zhong, JJ. (eds) Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9223-3_24

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