Abstract
The annual turnover of the herbal active substances exceeds yearly 10 billion Euros. The biotechnological production of these secondary metabolites leads year round supply of these compounds for the pharmaceutical use and it also helps to some extent to a conservation of natural resources in their countries of origin to avoid wild collections. Furthermore, the pharmaceutical production supports the product safety, constancy of price and quality of the secondary metabolites. During the past years, numerous different bioreactor types have been set up allowing a biotechnological production of secondary compounds in plants and the temporary immersion system is one among them which is considered as the simplest method. In this article, we have discussed the production of Digitalis purpurea shoot biomass using temporary immersion system.
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Abbreviations
- LEDs:
-
Light emitting diodes
- PET:
-
Polyethylene terephthalate
- TIS:
-
Temporary immersion system
References
Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851
Roca-PĂ©rez L, Boluda R, Gavidia I, PĂ©rez-BermĂºdez P (2004) Seasonal cardenolide production and Dop5βr gene expression in natural populations of Digitalis obscura. Phytochemistry 65:1869–1878
Vanisree M, Tsay HS (2004) Plant cell cultures – an alternative and efficient source for the production of biologically important secondary metabolites. Int J Appl Sci Eng 2:29–48
Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tiss Organ Cult 81:287–300
Wilken D, JimĂ©nez E, Hohe A, Jordan M, GĂ³mez R, Schmeda G, Gerth A (2005) Production of plant active compounds applying temporary immersion systems. In: Hvoslef-Eide AK, Preil W (eds) Liquid culture systems for in vitro plant propagation. Springer, Dordrecht, pp 525–537
Jeong C-S, Chakrabarty D, Hahn EJ, Lee H, Paek KY (2006) Effects of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots. Biochem Eng J 27:252–263
Russowski D, Maurmann N, Rech SB, Fett-Neto A (2006) Role of light and medium composition on growth and valepotriate contents in Valeriana glechomifolia whole plant liquid cultures. Plant Cell Tiss Organ Cult 86:211–218
Tisserat B, Vaughn S (2008) Growth, morphogenesis, and essential oil production in Mentha spicata L. plantlets in vitro. In Vitro Cell Dev Biol Plant 44:40–50
Quiala E, BarbĂ³n R, JimĂ©nez E, de Feria M, ChĂ¡vez M, Capote A, PĂ©rez N (2006) Biomass production of Cymbopogon citratus (D.C) Stapf., a medicinal plant, in temporary immersion systems. In Vitro Cell Dev Biol Plant 42:298–300
Georgiev V, Ilieva M, Bley T, Pavlov A (2008) Betalain production in plant in vitro systems. Acta Physiol Plant 30:581–593
Perez-Alonso N, Wilken D, Gerth A, Jahn A, Nitzsche HM, Kerns G, Capote-Perez A, Jiménez E (2009) Cardiotonic glycosides from biomass of Digitalis purpurea L. cultured in temporary immersion systems. Plant Cell Tiss Organ Cult 99:151–156
Levin R, Tanny G (2004) Bioreactors as low cost option for tissue culture. In: Low cost options for tissue culture technology in developing countries. International Atomic Energy Agency, Vienna, pp 47–54
Sivakumar G (2006) Bioreactor technology: a novel industrial tool for high-tech production of bioactive molecules and biopharmaceuticals form plant roots. Biotechnol J 1:1419–1427
Gupta SD, Ibaraki Y (2006) In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Dordrecht, p 477
Alvard D, Cote F, Teisson C (1993) Comparison of methods of liquid medium culture for banana micropropagation. Plant Cell Tiss Organ Cult 32:55–60
Albany N, JimĂ©nez E, Vilchez J, GarcĂa L, de Feria M, Perez N (2005) Use of growth retardants for banana shoot multiplication in TIS. In: Hvoslef-Eide AK, Preil W (eds) Liquid systems for in vitro mass propagation of plants. Springer, Dordrecht, pp 213–224
Roels S, Noceda C, Escalona M, Sandoval J, Canal MJ, RodrĂguez R (2006) The effect of headspace renewal in a temporary immersion bioreactor on plantain (Musa AAB) shoot proliferation and quality. Plant Cell Tiss Organ Cult 84:155–163
Haq I, Dahot MU (2007) Effect of immersion systems on chlorophyll contents in micropropagating banana. Afr J Biotechnol 6:1095–1101
Haq I, Dahot MU (2007) Micropropagation efficiency in banana (Musa sp.) under different immersion systems. Pak J Biol Sci 10:726–733
Aragon CE, Escalona M, RodrĂguez R, Canal MJ, Capote I, Pina D, Olmedo JG (2010) Effect of sucrose, light, and carbon dioxide on plantain micropropagation in temporary immersion bioreactors. In Vitro Cell Dev Biol Plant 46:89–94
Tisserat B, Vandercook CE (1985) Development of an automated plant culture system. Plant Cell Tiss Organ Cult 5:107–117
Teisson C, Alvard D (1995) A new concept of plant in vitro cultivation liquid medium: temporary immersion. In: Terzi M, Celia R, Falavigna A (eds) Current issues in plant molecular and cellular biology. Kluwer, Dordrecht, pp 105–110
Escalona M, Lorenzo JC, Gonzalez B, Daquinta M, Gonzalez JL, Desjardins Y, Borroto CG (1999) Pineapple (Ananas comosa L. Merr) micropropagation in temporary immersion systems. Plant Cell Rep 18:743–748
Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tiss Organ Cult 69:215–231
Jimenez E (2005) Mass propagation of tropical crops in temporary immersion systems: present status and future prospects. In: Hvoslef-Eide AK, Preil W (eds) Liquid systems for in vitro mass propagation of plants. Springer, Dordrecht, pp 197–211
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Wichtl M, Mangkudidjojo M, Wichtl-Bleier W (1982) Hochleistungsfluessigkeits-chromatographische Analyse von Digitalis-Blattextrakten. J Chromatogr 234:503–508
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Schumann, A., Claus, D., Gerth, A. (2014). In Vitro Production of Digitalis purpurea Biomass Using Temporary Immersion Cultures. In: Paek, KY., Murthy, H., Zhong, JJ. (eds) Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9223-3_17
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DOI: https://doi.org/10.1007/978-94-017-9223-3_17
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