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Circulating DNA and miRNA Isolation

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Part of the Advances in Predictive, Preventive and Personalised Medicine book series (APPPM, volume 5)

Abstract

Analysis of circulating nucleic acids undoubtedly represents a breakthrough in the diagnostic field and in predictive, preventive and personalized medicine. In order to adequately and systematically study and to transfer this approach into clinical practice, standardization of the pre-analytical steps is a crucial prerequisite. Thus, during the first pre-analytical step, it is critical to achieve nucleic acid extraction from blood cell free nucleic acid with the highest purity and yields. Optimization of isolation processes will lead to a low variation of measurements and sensitive quantification of these macromolecules that are often present at low concentration and sometimes are physically tightly associated with biological constituents in the blood. Various isolation methods are used, but ready to use extraction kits appear as a good compromise with respect to routine application, especially in a clinical setting. Improvement or high specificity of the circulating nucleic acid analysis might be possible with a better knowledge of their form and structure. The choice of the biological source (serum vs. plasma) is described in the previous chapter. Circulating DNA and microRNA were recently applied in clinical practice; their isolation methods are here described and discussed.

Keywords

Circulating DNA/RNA mRNA microRNA Isolation Plasma Serum 

Notes

Acknowledgements

Anthony Laybats for bibliographical work and Mr Batbedat for his financial support. The work was granted from the Fondation de la Recherche sur le Cancer (ARC). A.R. Thierry is supported by the Institut National de la Santé et de la Recherche Médicale (INSERM) (France).

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.IRCM-Institut de Recherche en Cancérologie de Montpellier, U896 INSERMMontpellierFrance
  2. 2.ICM-Institut de Cancérologie de MontpellierMontpellierFrance

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