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Nano-structuring for Molecular Motor Control

  • Mercy Lard
  • L. ten Siethoff
  • S. Kumar
  • M. Persson
  • G. te Kronnie
  • A. Månsson
  • H. Linke
Conference paper
Part of the NATO Science for Peace and Security Series B: Physics and Biophysics book series (NAPSB)

Abstract

The interaction of self-propelled biological molecular-motors and cytoskeletal filaments holds relevance for a variety of applications such as biosensing, drug screening, diagnostics and biocomputation. The use of these systems for lab-on-a-chip biotechnology applications shows potential for replacement of microfluidic flow by active, molecular-motor driven transport of filaments. The ability to control, confine and detect motile objects in such a system is possible by development of nanostructured surfaces for on-chip applications and fundamental studies of molecular-motors. Here we describe the localized detection (Lard et al., Sci Rep 3:1092, 2013) and fast transport of actin filaments by myosin molecular-motors (Lard et al., Biosens Biolectron 48(0):145–152, 2013), inserted within nanostructures, as a method for biocomputation and molecular concentration. These results include extensive myosin driven concentration of actin filaments on a miniaturized detector, of relevance for use of molecular-motors in a diagnostics platform. Also, we discuss the local enhancement of the fluorescence signal of filaments, relevant for use in a biocomputation device where tracking of potentially thousands of motile objects is of primary significance.

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Mercy Lard
    • 1
  • L. ten Siethoff
    • 2
  • S. Kumar
    • 2
    • 3
  • M. Persson
    • 2
  • G. te Kronnie
    • 4
  • A. Månsson
    • 2
  • H. Linke
    • 1
  1. 1.The Nanometer Structure Consortium (nmC@LU), Division of Solid State PhysicsLund UniversityLundSweden
  2. 2.Department of Chemistry and Biomedical SciencesLinnaeus UniversityKalmarSweden
  3. 3.Department of BiotechnologyDelhi Technological UniversityDelhiIndia
  4. 4.Department of Women’s and Children’s HealthUniversity of PadovaPadovaItaly

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