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Octopus vulgaris. Paralarval Culture

  • José IglesiasEmail author
  • Lidia Fuentes
Chapter

Abstract

There have been many attempts worldwide to produce in captivity juveniles of Octopus vulgaris, one the most studied cephalopod species in the world because of its very strong market interest. This chapter reviews the different methods used to obtain and maintain broodstocks and the rearing technologies applied to the paralarvae. The main parameters and culture methods to rear the planktonic stage are discussed and a protocol for the rearing of the paralarvae is suggested. The main bottlenecks in the cultivation of this species are emphasized, and further research topics are suggested, including both technical and biological aspects.

In laboratory trials, the best growth and survival of the paralarval phase is currently achieved by feeding a mixed live diet composed of enriched Artemia and crustacean zoeae. However, this method is not transferable to a commercial scale as there is limited availability of live zoeae. In order to advance from a research to an industrial scale, it is essential to develop an inert diet with the appropriate nutritional composition to be supplied from an age of 1 month onwards. Another option would be to develop an appropriate enrichment protocol for Artemia so that its composition simulates more closely that of crustacean larvae or wild zooplankton.

A protocol for the first month of O. vulgaris paralarvae culture, which allows the production of good-quality individuals (in terms of dry weight and survival) to start the settlement process, is proposed. Relatively high survival rates and paralarvae dry weights of 1.3–1.8 mg can be attained after 1 month on a sole diet of Artemia. These weights are increased to 2.5–3.5 mg when that diet is supplemented with zoeae.

Keywords

Octopus vulgaris Common octopus Paralarvae rearing Paralarvae feeding Culture conditions 

References

  1. Almansa E, Shcherbakova A, Jiménez P, Rodríguez C, Riera R, Felipe BC, Martín MV, Andrade JP, Sykes A (2012) Effects of different tank volumes and feeding regimes on growth, survival and lipid composition of Octopus vulgaris paralarvae. Book of abstracts, Aqua 2012. Czech Republic, Prague, p 52Google Scholar
  2. Arai D, Kurihara A, Komi R, Iwamoto A, Takeuchi T (2008) Effect of feeding various amounts of Pacific sandeel flakes on growth survival and carcass fatty acid composition of common octopus Octopus vulgaris paralarvae. Aquac Sci 56(4):595–600Google Scholar
  3. Bersano JGF (2003) Intensive cultivation of the calanoid copepod Acartia tonsa. A potential source of live food for aquaculture. In: Book of abstracts, proceedings: word aquaculture, p 95, Salvador, BA, Brazil, 19–23 May 2003Google Scholar
  4. Boletzky SV (1987) Embryonic phase. In: Boyle PR (ed) Cephalopod life cycles, vol 2. Academic Press, London, pp 5–31Google Scholar
  5. Carrasco JF, Rodríguez C, Rodríguez M (2005) Cultivo intensivo de paralarvas de pulpo (Octopus vulgaris, Cuvier) utilizando como base de la alimentación zoeas vivas de crustáceos. Libro de actas, IX Congreso Nacional de Acuicultura, Sevilla, pp 219–222Google Scholar
  6. Carrasco JF, Arronte JC, Rodríguez C (2006) Paralarval rearing of the common octopus, Octopus vulgaris (Cuvier). Aquac Res 37:1601–1605CrossRefGoogle Scholar
  7. Cerezo Valverde J, García GB (2005) Suitable dissolved oxygen for common octopus (Octopus vulgaris, cuvier, 1797) at different weights and temperatures: analysis of respiratory behaviour. Aquac 244:303–314CrossRefGoogle Scholar
  8. Couto A (2012) Study on the wild diet of Octopus vulgaris paralarvae in Ría de Vigo (NW Spain). Dissertation application to the Master’s degree in marine sciences and marine resources submitted to Institute of Biomedical Sciences Abel Salazar, University of Porto. pp 1–90Google Scholar
  9. De Wolf T, Lenzi S, Lenzi F (2011) Paralarval rearing of Octopus vulgaris (Cuvier) in Tuscany, Italy. Aquac Res 42:1406–1414CrossRefGoogle Scholar
  10. Estefanell JA (2012) Optimización de las condiciones de engorde y avances en el conocimiento de los requerimientos nutricionales del pulpo común Octopus vulgaris (Cuvier, 1797). Doctoral Thesis, Universidad de Las Palmas de Gran Canaria, Gran Canaria, p 292Google Scholar
  11. Estévez A, Gairin I, Berger E (2009) Wild zooplancton for Octopus vulgaris larval rearing. In: Hendry CI, Van Stappen G, Wille M, Sorgeloos P (eds) LARVI 09, Fish & shellfish larviculture symposium, special publication No. 38. European Aquaculture Society, Oostende, pp 88–91Google Scholar
  12. FAO (2012). FAO yearbook. Fishery and aquaculture statistics. 2010. Rome, pp 609Google Scholar
  13. Feyjoo P, Riera R, Felipe CB, Skalli A, Almansa E (2011) Tolerance response to ammonia and nitrite in paralarvae of Octopus vulgaris and its toxic effects on prey consumption rate and chromatophores activity. Aquac Int 19(1):193–204CrossRefGoogle Scholar
  14. Fuentes L, Iglesias J, Sánchez FJ, Otero JJ, Moxica C, Lago MJ (2005) Técnicas de transporte de paralarvas y adultos de pulpo (Octopus vulgaris). Bol Inst Esp Oceanogr 21(1–4):155–162Google Scholar
  15. Fuentes L, Sánchez FJ, Otero JJ, Lago MJ, Iglesias J (2009) Utilización de zooplancton natural y Artemia en el cultivo de paralarvas de pulpo Octopus vulgaris. Libro de resúmenes, Congreso Nacional de Acuicultura. Madrid, pp 122–123Google Scholar
  16. Fuentes L, Sánchez FJ, Lago MJ, Iglesias J, Pazos G, Linares F (2011) Growth and survival of Octopus vulgaris (Cuvier 1797) paralarvae fed on 3 Artemia-based diets complemented with frozen fish flakes, crushed zooplankton and marine microalgae. Sci Mar 75(4):771–777CrossRefGoogle Scholar
  17. Garrido D, Reis D, Orol D, Gonçalves R, Martín MV, Sykes AV, Rodríguez C, Felipe BC, Santamaría FJ, Zheng X, Almansa E (2012) Efecto de distintos tipos de iluminación en el cultivo de las paralarvas del pulpo común (Octopus vulgaris Cuvier, 1797). In: V Foro Iberoamericano de los Recursos Marinos y la Acuicultura, Cádiz, 26–29 Nov 2012Google Scholar
  18. Gunnarsli K, Toften H, Mortensen A (2008) Effects of nitrogen gas supersaturation on growth and survival in juvenile Atlantic cod (Gadus morhua L.). Aquac 283:175–179. http://dx.doi.org/10.1016/j.Aquac. Accessed 29 June 2008Google Scholar
  19. Hamasaki K, Morioka T (2002) Effects of temperature on egg incubation period, paralarval survival and growth of common octopus, Octopus vulgaris reared in the laboratory. Suisanzoshoku 50:407–413Google Scholar
  20. Hamasaki K, Takeuchi T (2000) Effects of the addition of Nannochloropsis to the rearing water on survival and growth of planktonic larvae of Octopus vulgaris. Saibai- Giken 28:65–68Google Scholar
  21. Hamasaki K, Takeuchi T (2001) Dietary value of Artemia enriched with ω-yeast or shark eggs as feed for planktonic larvae of Octopus vulgaris. Saibai-Giken 28:13–16Google Scholar
  22. Hamasaki H, Fukunaga K, Yoshida Y, Maruyama K (1991) Effects of marine microalgae Nannochloropsis sp. on survival and growth on rearing pelagic paralarvae of Octopus vulgaris, and results of mass culture in the tank of 20 metric tons. Saibai-Giken 19:75–84Google Scholar
  23. Hargreaves JA, Tucker CS (1999) Design and construction of degassing units for Catfish hatcheries. Southern Regional Aquaculture Center, publication No. 191. Mississippi State UniversityGoogle Scholar
  24. Hormiga JA, Almansa E, Sykes AV, Torres NV (2010) Model based optimization of feeding regimens in aquaculture: application to the improvement of Octopus vulgaris viability in captivity. J Biotechnol 149:209–214. doi: 10.1016/j.jbiotec.2009.12.008CrossRefGoogle Scholar
  25. Iglesias J, Fuentes L (2013) Cephalopods paralarval rearing with special reference to the common octopus (Octopus vulgaris, Cuvier 1797). In: Allan G, Burnell G (eds) Advances in aquaculture hatchery technology, Number 242. Woodhead Publishing Series in Food Science, Technology and Nutrition, Oxford, pp 374–403Google Scholar
  26. Iglesias J, Sánchez FJ, Otero JJ (1997) Primeras experiencias sobre el cultivo integral del pulpo (Octopus vulgaris) en el Instituto Español de Oceanografía. In Costa J, Abellan E, García B, Ortega A, Zamara S (eds) Libro de actas, VII Congreso Nacional de Acuicultura. Cartagena, pp 221–226, ISBN: 84-491-0323Google Scholar
  27. Iglesias J, Sánchez FJ, Otero JJ, Moxica C (2000) Culture of octopus (Octopus vulgaris, Cuvier). Present knowledge, problems and perspectives. Cah Options Méditer 47:313–321Google Scholar
  28. Iglesias J, Otero JJ, Moxica C, Fuentes L, Sánchez FJ (2004) The completed life cycle of the octopus (Octopus vulgaris, Cuvier) under culture conditions: paralarval rearing using Artemia and zoeae, and first data on juvenile growth up to 8 months of age. Aquac Int 12:481–487CrossRefGoogle Scholar
  29. Iglesias J, Fuentes L, Sánchez FJ, Otero JJ, Moxica C, Lago MJ (2006) First feeding of Octopus vulgaris Cuvier, 1797 paralarvae using Artemia: effect of prey size, prey density and feeding frequency. Aquac 261(2):817–822CrossRefGoogle Scholar
  30. Iglesias J, Sánchez FJ, Bersano JGF, Carrasco JF, Dhont J, Fuentes L, Linares F, Muñoz JL, Okumura S, Roo J, van der Meeren T, Vidal EAG, Villanueva R (2007) Rearing of Octopus vulgaris paralarvae: Present status, bottlenecks and trends. Aquac 266:1–15CrossRefGoogle Scholar
  31. Imamura S (1990) Larval rearing of octopus (Octopus vulgaris Cuvier). The progress of technological development and some problems remained. Collect Breed 52:339–343Google Scholar
  32. Itami K, Izawa Y, Maeda S, Nakai K (1963) Notes on the laboratory culture of the Octopus larvae. Bull Jap Soc Sci Fish 29(6):514–520CrossRefGoogle Scholar
  33. Kurihara A, Okumura S, Iwamoto A, Takeuchi T (2006) Feeding Pacific sandeel enhances DHA level in common octopus paralarvae. Aquac Sci 54:413–420Google Scholar
  34. Lenzi F, Cittolin G, Ingle E, Tibaldi E (2002) Allevamento del polpo (Octopus vulgaris): riproduzione e allevamento larvale in avannotteria industriale. Ricerca per lo sviluppo dell’Aquacoltura Toscana, risultati conseguiti, pp 73–83Google Scholar
  35. Lenzi F, Capiferri U, De Wolf T (2006) Paralarval rearing of the common Octopus Octopus vulgaris: state of the art in Italy. In: Book of abstracts Aqua, Firenze, Florence, Italy, p 523, 9–13 May 2006Google Scholar
  36. Mangold K (1983) Octopus vulgaris. In: Boyle PR (ed) Cephalopod life cycles, vol I. Academic Press, London, pp 335–364Google Scholar
  37. Mangold K, Boletzky S (1973) New data on reproductive biology and growth of Octopus vulgaris. Mar Biol 19:7–12CrossRefGoogle Scholar
  38. Márquez L, Quintana D, Lorenzo A, Almansa E (2013). Biometrical relationships in developing eggs and neonates of Octopus vulgaris in relation to parental diet. Helgoland Marine ResearchGoogle Scholar
  39. Moxica C, Linares F, Otero JJ, Iglesias J, Sánchez FJ (2002) Cultivo intensivo de pararlarvas de pulpo, Octopus vulgaris Cuvier, 1797, en tanques de 9 m3. Bol Inst Esp Oceanogr 18(1–4):31–36Google Scholar
  40. Moxica C, Fuentes L, Hernández J, Iglesias J, Lago MJ, Otero JJ, Sánchez FJ (2006) Efecto de Nannochloropsis sp. en la supervivencia y crecimiento de paralarvas de pulpo Octopus vulgaris. IX Foro dos Recursos Mariños e da Acuicultura das Rías Gallegas 9:255–261Google Scholar
  41. Naef A (1928) Cephalopoda embryology, part I, vol II (final part of monograph no. 35). In: Fauna and flora of the Bay of Naples (translated by the Smithsonian Institution Libraries). Smithsonian Institution Libraries, Washington 35:1–461Google Scholar
  42. Navarro JC, Villanueva R (2000) Lipid and fatty acid composition of early stages of cephalopods: an approach to their lipid requirements. Aquac 183:161–177CrossRefGoogle Scholar
  43. Navarro JC, Villanueva R (2003) The fatty acid composition of Octopus vulgaris paralarvae reared with live and inert food: deviation from their natural fatty acid profile. Aquac 219:613–631CrossRefGoogle Scholar
  44. Okumura S, Kurihara A, Iwamoto A, Takeuchi T (2005) Improved survival and growth in Octopus vulgaris paralarvae by feeding large type Artemia and Pacific sandeel, A. personatus. Aquac 244:144–157CrossRefGoogle Scholar
  45. Parra G, Villanueva R, Yúfera M (2000) Respiration rates in late eggs and early hatchlings of the common octopus, Octopus vulgaris. J Mar Biol Ass UK 80:557–558CrossRefGoogle Scholar
  46. Quintana D (2009) Valoración de los requerimientos nutricionales de reproductores de pulpo común Octopus vulgaris. PhD Thesis, Universidad de La Laguna, Tenerife, p 225Google Scholar
  47. Quintana D, Márquez L, Arévalo JR, Almansa E, Lorenzo A (2007) Aplicación de análisis multivariante al estudio de la influencia de la dieta de los reproductores de Octopus vulgaris sobre la composición lipídica de huevos y paralarvas y su relación con la calidad de puesta. Poster. XI Congreso Nacional de Acuicultura, Vigo, Sept 2007Google Scholar
  48. Quintana D, Márquez L, Suárez H, Rodríguez E, Jerez S, Almansa E (2009) Efecto de la dieta de los reproductores de pulpo común (Octopus vulgaris) sobre la composición de aminoácidos de huevos y paralarvas: Relación con la calidad de puesta. XII Congreso Nacional de Acuicultura, MadridGoogle Scholar
  49. Rosas C, Caamal C, Cazares RJ (2010) Incubation process for octopuses and incubator. Universidad Autónoma de México. Organización Mundial de la Propiedad Intelectual. WO 2010/030155 A1Google Scholar
  50. Roura A, González AF, Redd K, Guerra A (2012) Molecular prey identification in wild Octopus vulgaris paralarvae. Mar Biol 159(6):1335–1345CrossRefGoogle Scholar
  51. Sánchez FJ, Fuentes L, Otero JJ, Lago MJ, Linares F, Pazos G, Iglesias J (2010) Effect of tank volume on the growth and survival of reared Octopus vulgaris paralarvae. In: Aquaculture Europe, CD abstracts, Porto, pp 1170–1171Google Scholar
  52. Seixas P (2009) Composición bioquímica y crecimiento de paralarvas de pulpo (Octopus vulgaris Cuvier, 1797), alimentadas con juveniles de Artemia enriquecidos con microalgas y otros suplementos nutricionales. PhD Thesis, University of Santiago de Compostela, p 279. ISBN: 978-84-9887-253–8Google Scholar
  53. Seixas P, Rey-Méndez M, Valente LMP, Otero A (2010) High DHA content in Artemia is ineffective to improve Octopus vulgaris paralarvae rearing. Aquac 300:156–162CrossRefGoogle Scholar
  54. Skiftesvik AB, Browman HI, St-Pierre JF (2003) Life in green water: the effect of microalgae on the behaviour of Atlantic cod (Gadus morhua) larvae. In: 26th annual larval fish conference, Norway OS, 22–26 July 2002. In: Browman HI, Skiftesvik AB (eds) Big fish bang, pp 97–103Google Scholar
  55. Sykes AV, Baptista FD, Gonçalves RA, Andrade JP (2012) Directive 2010/63/EU on animal welfare: a review on the existing scientific knowledge and implications in cephalopod aquaculture research. Rev Aquac 4:142–162CrossRefGoogle Scholar
  56. Uriarte I, Iglesias J, Domingues P, Rosas C, Viana MT, Navarro JC, Seixas P, Vidal E, Ausburger A, Pereda S, Godoy F, Paschke K, Farías A, Olivares A, Zúñiga O (2011) Current status and bottleneck of octopod aquaculture: the case of American species. J World Aquac Soc 42(6):735–752CrossRefGoogle Scholar
  57. Vaz-Pires P, Seixas P, Barbosa A (2004) Aquaculture potential of the common octopus (Octopus vulgaris Cuvier, 1797): a review. Aquac 238(1–4):221–238CrossRefGoogle Scholar
  58. Viciano E, Iglesias J, Lago MJ, Sánchez FJ, Otero JJ, Navarro JC (2011) Fatty acid composition of polar and neutral lipid fractions of Octopus vulgaris Cuvier, 1797 paralarvae reared with enriched on-grown Artemia. Aquac Res 42:704–709CrossRefGoogle Scholar
  59. Vidal EAG, DiMarco FP, Wormuth JH, Lee PG (2002) Optimizing rearing conditions of hatchling loliginid squid. Mar Biol 140:117–127CrossRefGoogle Scholar
  60. Vidal et al (manuscript in elaboration) In: Vidal E, Villanueva R (eds) Cephalopod culture: current status on main biological models and research priorities. Adv Mar BiolGoogle Scholar
  61. Villanueva R (1994) Decapod crab zoeae as food for rearing cephalopod paralarvae. Aquac 128:143–152CrossRefGoogle Scholar
  62. Villanueva R (1995) Experimental rearing and growth of planktonic Octopus vulgaris from hatching to settlement. Can J Fish Aquat Sci 52:2639–2650CrossRefGoogle Scholar
  63. Villanueva R, Bustamante P (2006) Composition in essential and non-essential elements of early stages of cephalopods and dietary effects on the elemental profiles of Octopus vulgaris. Aquac 261:225–240CrossRefGoogle Scholar
  64. Villanueva R, Norman MD (2008) Biology of the planktonic stages of benthic octopuses. In: Gibson RN, Gordon JDM (eds) Oceanography and marine biology: an annual review, vol 46. CRC Press, pp 105–202Google Scholar
  65. Villanueva R, Koueta N, Riba J, Boucaud-Camou E (2002) Growth and proteolytic activity of Octopus vulgaris paralarvae with different food rations during first-feeding, using Artemia nauplii and compound diets. Aquac 205:269–286CrossRefGoogle Scholar
  66. Villanueva R, Riba J, Ruíz-Capillas C, González AV, Baeta M (2004) Amino acid composition of early stages of cephalopods and effect of amino acid dietary treatments on Octopus vulgaris paralarvae. Aquac 242:455–478CrossRefGoogle Scholar
  67. Villanueva R, Escudero JM, Deulofeu R, Bozzano A, Casoliva C (2009) Vitamin A and E content in early stages of cephalopods and their dietary effects in Octopus vulgaris paralarvae. Aquac 286:277–282CrossRefGoogle Scholar
  68. Young RE, Harman RF (1988) Larva, paralarvae and subadult in cephalopod terminology. Malacologia 29:201–207Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Oceanographic Center of VigoInstituto Español de Oceanografía (IEO)VigoSpain

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