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Production of Olfactory Receptors and Nanosomes Using Yeast System for Bioelectronic Nose

  • Marie-Annick PersuyEmail author
  • Guenhaël Sanz
  • Aurélie Dewaele
  • Christine Baly
  • Edith Pajot-Augy
Chapter

Abstract

Olfactory receptors (ORs) constitute the largest multigenic G protein-coupled receptor family, and are involved in the recognition of thousands of odorant molecules. However, if most of these ORs are identified on the basis of their DNA sequences, they are still unmatched to their natural ligands, and their deorphanization remains a challenging bottleneck. To identify odorant-olfactory receptor pairs, various techniques have been developed to clone and produce ORs in cells, to allow the screening of their response to odorants. During the last decade, various ORs were successfully expressed in S. cerevisiae, and many improvements achieved. This chapter reviews the latest developments of yeast-based technologies to produce ORs and test their functional response.

Keywords

Green Fluorescent Protein Yeast Cell Functional Response Olfactory Receptor Induction Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Marie-Annick Persuy
    • 1
    Email author
  • Guenhaël Sanz
    • 1
  • Aurélie Dewaele
    • 1
  • Christine Baly
    • 1
  • Edith Pajot-Augy
    • 1
  1. 1.INRA, UR1197 NeuroBiologie de l’OlfactionJouy-en-JosasFrance

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