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Production of Olfactory Receptors and Nanosomes Using Yeast System for Bioelectronic Nose

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Bioelectronic Nose

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Abstract

Olfactory receptors (ORs) constitute the largest multigenic G protein-coupled receptor family, and are involved in the recognition of thousands of odorant molecules. However, if most of these ORs are identified on the basis of their DNA sequences, they are still unmatched to their natural ligands, and their deorphanization remains a challenging bottleneck. To identify odorant-olfactory receptor pairs, various techniques have been developed to clone and produce ORs in cells, to allow the screening of their response to odorants. During the last decade, various ORs were successfully expressed in S. cerevisiae, and many improvements achieved. This chapter reviews the latest developments of yeast-based technologies to produce ORs and test their functional response.

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Authors and Affiliations

  1. INRA, UR1197 NeuroBiologie de l’Olfaction, 78350, Jouy-en-Josas, France

    Marie-Annick Persuy, Guenhaël Sanz, Aurélie Dewaele, Christine Baly & Edith Pajot-Augy

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  1. Marie-Annick Persuy
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  2. Guenhaël Sanz
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  3. Aurélie Dewaele
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  4. Christine Baly
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  5. Edith Pajot-Augy
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Correspondence to Marie-Annick Persuy .

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Editors and Affiliations

  1. School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea, Republic of (South Korea)

    Tai Hyun Park

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Persuy, MA., Sanz, G., Dewaele, A., Baly, C., Pajot-Augy, E. (2014). Production of Olfactory Receptors and Nanosomes Using Yeast System for Bioelectronic Nose. In: Park, T. (eds) Bioelectronic Nose. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8613-3_8

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