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Engineering Hydrogenases for H2 Production: Bolts and Goals

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Book cover Microbial BioEnergy: Hydrogen Production

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 38))

Summary

Hydrogenases are efficient biological catalysts of H2 oxidation and production. Most of them are inhibited by oxygen, and a prerequisite for their use in biotechnological applications under air is to improve their oxygen tolerance. A few bacteria, however, contain hydrogenases that activate H2 even in the presence of O2. Intriguingly, molecular, kinetic and spectroscopic studies lead to assume that different mechanisms might be responsible for the resistance, depending on the enzyme type. In order to better understand the molecular bases of resistance to O2 inhibition, this chapter focuses on the hydrogenases and their reaction with O2 and examines the different strategies to lead to engineer kinetically efficient hydrogenases operating under aerobic conditions.

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Abbreviations

DFT:

Density function theory;

ENDOR:

Electron nuclear double resonance;

EPR:

Electron paramagnetic resonance;

EXAFS:

Extended X-Ray absorption fine structure;

Fd:

Ferredoxin;

FHL:

Formate hydrogen lyase;

FNR:

Ferredoxin NADPH reductase;

FTIR:

Fourier Transform InfraRed spectroscopy;

Hmd:

H2-forming methylenetetrahydromethanopterin dehydrogenase;

MBH:

Membrane-bound hydrogenase;

PFV:

Protein film voltammetry;

RH:

Regulatory hydrogenase;

SH:

Soluble hydrogenase;

SHE:

Standard hydrogen electrode;

WT:

Wild type;

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Authors and Affiliations

  1. CNRS, BIP, 31 chemin Joseph Aiguier, 13402, Marseille Cedex 20, France

    Marc Rousset

  2. IRD, Aix-Marseille Université, CNRS/INSU, MIO, UM 110, 13288, Marseille Cedex 09, France

    Pierre-Pol Liebgott

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  1. Dept. of Pharmacy and BioTechnology, University of Bologna, Bologna, Italy

    Davide Zannoni

  2. Dept. of AgriFood Production and Environmental Sciences, University of Florence, Florence, Italy

    Roberto De Philippis

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Rousset, M., Liebgott, PP. (2014). Engineering Hydrogenases for H2 Production: Bolts and Goals. In: Zannoni, D., De Philippis, R. (eds) Microbial BioEnergy: Hydrogen Production. Advances in Photosynthesis and Respiration, vol 38. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8554-9_3

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