Abstract
Conditional on preparation techniques, stem cell products contain a considerable amount of platelets. But platelets are the major source of soluble CD40 ligand (sCD40L) in the blood. It has been demonstrated that CD40L is cleaved from the surface of activated platelets. So it can be expected that sCD40L accumulates in stem cell products reaching levels above the physiological range.
Therefore sCD40L concentrations were observed in peripheral blood samples and in the respective stem cell products of allogenic stem cell donors (peripheral stem cell apheresis or bone marrow) as well as of patients undergoing autologous stem cell apheresis. sCD40L concentrations were normally determined by different ELISA techniques.
In stem cell products, sCD40L concentrations were manifold elevated (range from 1,239 to 3,839 pg/mL) in comparison to concentrations of peripheral blood samples (range from 43 to 321 pg/mL). Experimentally, using MMP-9-, MMP-2- and MMP-2/9-inhibitors sCD40L release by platelets could be inhibited up to 90% of the control values. During autologous stem cell apheresis, the decrease in platelet count from 95,070/μL ± 58,234/μL at the beginning to 54,638/μL ± 26,944/μL at the end of the procedure was accompanied by a significant lowering of sCD40L concentrations in peripheral blood samples from 239 pg/mL ± 139 pg/mL to 126 pg/mL ± 71 pg/mL (dependent on platelet count, linearly correlated, r = 0.95).
As known from platelet concentrates, an accumulation of sCD40L could also be observed in stem cell products pointing out the importance of sCD40L release by platelets. In addition, during stem cell apheresis sCD40L concentrations in peripheral blood were mainly influenced by alterations of platelet count.
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Wenzel, F. (2014). Soluble CD40L in Stem Cell Products. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 12. Stem Cells and Cancer Stem Cells, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-8032-2_21
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DOI: https://doi.org/10.1007/978-94-017-8032-2_21
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