Abstract
In this chapter, we gave the detailed protocols for studies of bypassing DNA damage using a DNA polymerase. These methods include kinetic analysis, LC-MS/MS sequence analysis of full-length extension products beyond DNA damage, and X-ray crystal structure analysis of DNA polymerase containing DNA damage. Kinetic analysis contains steady-state kinetic analysis of single dNTP incorporation against DNA damage, full-length extension beyond DNA damage, pre-steady-state kinetic analysis of dNTP incorporation, and kinetic analysis of conformational change. This analysis gave details about how a DNA polymerase bypasses DNA damage.
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Zhang, H. (2015). Protocols for Studies of Bypass of DNA Damage by DNA Polymerase. In: DNA Replication - Damage from Environmental Carcinogens. SpringerBriefs in Biochemistry and Molecular Biology, vol 17. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-7212-9_6
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DOI: https://doi.org/10.1007/978-94-017-7212-9_6
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