Random amplified polymorphic DNA (RAPD) markers
Williams  and Welsh and McClelland  demonstrated the utility of single short oligonucleotide primers of arbitrary sequence for the amplification of DNA segments distributed randomly throughout the genome. Welsh and McClelland showed that the pattern of amplified bands could be used for genome fingerprinting  and Williams et al.  showed that the differences (polymorphisms) in the pattern of bands amplified from genetically distinct individuals behaved as mendelian genetic markers (named RAPDs, for Random Amplified Polymorphic DNA). Well-saturated maps of the Arabidopsis  and pine  genomes have been constructed using RAPD technology. A single set of arbitrary-sequence 10-mers may be used for mapping or fingerprinting any species. The many advantages of RAPD markers over RFLPs or isozyme markers accelerated the adoption of RAPD technology for the construction of genetic maps, fingerprinting, and population genetic studies . Current reviews of the applications of RAPD technology are available [10, 12].
KeywordsSomatic Embryo Recombinant Inbred Stoffel Fragment
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- 1.Anon. (1991) User Manual GenAmp PCR System 9600. Norwalk, CT: Perkin Elmer Cetus.Google Scholar
- 4.Grattapaglia D, Chaparro J, Wilcox P, McCord S, Werner D, Amerson H, McKeand S, Bridgwater S, Whetten R, O’Malley D, Sederoff R (1992) Mapping in woody plants with RAPD markers: Application to breeding in forestry and horticulture. In: Proceedings of the Symposium ‘Application of RAPD Technology to Plant Breeding’, Joint Plant Breeding Symposia Series, Crop Science Society of America/American Society for Horticultural Science/American Genetic Association, November 1, 1992, Minneapolis, MN.Google Scholar
- 5.Dellaporta SL, Wood J, Hicks JB (1985) Maize DNA Miniprep. In: Molecular Biology of Plants, pp. 36–37. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.Google Scholar
- 7.Horn P, Rafalski, A (1992) Non-destructive RAPD Genetic diagnostics of microspore-derived Brassica embryos. Plant Mol Biol Rep 10: 281–289.Google Scholar
- 8.Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning. A Laboratory Manual. (1st ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.Google Scholar
- 10.Rafalski JAV, Tingey S, Williams JGK (1991) RAPD markers — a new technology for genetic mapping and plant breeding. AgBiotech News and Information 3: 645–648.Google Scholar
- 15.Williams JGK, Rafalski JA, Tingey SV (1993) Genetic analysis using RAPD markers. In: R. Wu (Eds.) Methods in Enzymology. Orlando, FL: Academic Press. Vol. 218, 704–740.Google Scholar