Summary
At least 4 zones of amylase activity were distinguished after starch-agar electrophoresis of swine serum. One zone was situated in the leading edge of serum albumin and three zones in the γ-globulin area. Two-dimensional electrophoresis, starch-agar-starch gel, demonstrated that the amylase of the albumin field migrates in starch gel and exhibits gene-controlled variations, while the amylase of the γ-field apparently remain at the insertion point.
Various tissues were subjected to starch-agar and starch gel electrophoresis for the separation of amylase. The majority of tissue amylase investigated appeared in the γ-globulin field. The amylases in the follicular fluid and uterus extracts showed, however, an electrophoretic picture identical to that of serum. Finally, the tissue source of serum amylase and the state of the various amylases in serum are discussed.
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References
Aronsson, T. and Grønvall, A,, 1957, Improved Separations of serum proteins in paper electrophoresis. A new electrophoresis buffer, Scand. J. Clin. Lab. Invest., 9, 338–341.
Ashton, G.C., 1960. “Thread protein” and ß-globulin polymorphism in the serum proteins of pigs, Nature (Lond.), 186, 991–992.
Ashton, G. C, 1965. Serum amylase (thread protein) polymorphism in cattle, Genetics (Princeton), 51, 431–437.
Berk, J. E. and Searcy, R. L., 1965. Isoenzymes of serum amylase in man, Gastroenterology, 48, 651–653.
Dreiling, D. A., Janowitz, H.D. and Josephberg, L.J., 1963. Serum isoamylases. An electro-phoretic study of the blood amylase and the patterns observed in pancreatic disease, Ann. Intern. Med., 58, 235–244.
Graetzer, M. A., Hesselholt, M., Moustgaard, J. and Thymann, M., 1964. Studies on protein polymorphism in pigs, horses and cattle. Blood Groups of Animals, Proc. 9 Europ. Anim. Blood Groups Conf, Prague, 1965, 279–293.
Hesselholt, M., 1966. Additional serum type alleles in pigs (In Danish, English summary), Aars-beretning. Inst. Sterilitetsforskn, Copenhagen, 75–83.
Hesselholt, M., Larsen, B. and Nielsen, P. B., 1966. Studies on serum amylase systems in swine, horses and cattle, Aarsskr. K. Vet. Landbohøjsk, 78–90.
Hesselholt, M. and Moustgaard, J., 1965. Serum amylase polymorphism in cattle and its application in the parentage control (In Danish, English summary). Aarsberetn. Inst. Sterilitets-forsk, Copenhagen, 175–182.
Hirschfeld, J. 1960. Immunoelectrophoresis — Procedure and application to the study of group-specific variations in sera, Sci. Tools, 7, 18–25.
Imlah, P., 1964. A study of blood groups in pigs. Blood Groups of Animals, Proc. 9 Europ. Anim. Blood Groups Conf., Prague, 1965, 109–122.
Latner, A. L., 1965. The binding of circulating enzymes by plasma proteins, West-Europ. Symp. on Clin. Chem, Vol.5, 121–127.
Mc Geachin, R. L. and Lewis, J. P., 1959. Electrophoretic behaviour of serum amylase, J. Biol. Chem., 234, 795–798.
Poulik, M. D. and Smithies, O., 1958. Comparison and combination of the starch-gel and filter-paper electrophoretic methods applied to human sera: Two-dimensional electrophoresis, Bio-chem. J., 68, 636–643.
Schröffel, J. and Hojný, J., 1962. β-globulin fractions in pig serum and their heredity (In Czech. Abstract), Cs. fysiologie, 11, 277.
Wilding, P., 1963. Use of gel filtration in the study of human amylase, Clin. Chim. Acta, 8, 918–924.
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Hesselholt, M. (1970). Additional Studies into Serum Amylase in Swine. In: XIth European Conference on Animal Blood Groups and Biochemical Polymorphism. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-5226-8_54
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DOI: https://doi.org/10.1007/978-94-017-5226-8_54
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