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Structure and Functional Studies of an Oxygen-evolving Photosystem II Preparation

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Part of the book series: Advances in Agricultural Biotechnology ((AABI,volume 3))

Abstract

Photosystem II(PSII) provides the electrons for plastoquinone reduction and the positive charges for water oxidation. The secondary electron transfer events associated with PSII, such as plastoquinone reduction and water oxidation, remain to be elucidated. In the present work, we have examined the composition and functional properties of a resolved PSII preparation capable of oxygen evolution and plastoquinone photoreduction. Because this preparation is devoid of PSI and deficient in the cytochrome b/f complex, it is ideal for detailed structural analysis.

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References

  • Argyroudi-Akoyunoglou J and Thomou H (1981) Separation of thylakoid pigment protein complexes by SDS-sucrose density gradient centrifugation, FEBS Lett 135, 177–181.

    Article  CAS  Google Scholar 

  • Berthold DA, Babcock GT and Yocum CF (1981) A highly resolved, oxygenevolving photosystem II preparation from spinach thylakoid membranes, FEBS Lett. 134, 231–234.

    Article  CAS  Google Scholar 

  • Chua N-H (1980) Electrophoretic analysis of chloroplast proteins, Methods in Enzymology 69, 434–446.

    Article  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680–682.

    Article  PubMed  CAS  Google Scholar 

  • Lam E, Baltimore B, Ortiz W, Chollar S, Melis A and Malkin R (1983) Characterization of a resolved oxygen-evolving photosystem II preparation frorr spinach thylakoids, Biochim. Biophys. Acta, in the press.

    Google Scholar 

  • Melis A and Duysens LNM (1979) Biphasic energy conversion kinetics and absorbance difference spectra of photosystem II of chloroplasts, Photobiochem Photobiol. 29, 373–382.

    Article  CAS  Google Scholar 

  • Melis A and Hart RW (1980) A laboratory-constructed sensitive difference spectrophotometer for the ultraviolet, visible, and far-red region of the spectrum. In Carnegie Institution of Washington year book of 1979, pp. 170–172. Stanford, California; Carnegie Institution.

    Google Scholar 

  • Mullet JE and Arntzen CJ (1981) Identification of a 32–34 kilodalton polypeptide as a herbicide receptor protein in photosystem II, Biochim. Biophys Acta 635, 236–248.

    Article  PubMed  CAS  Google Scholar 

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© 1984 Springer Science+Business Media Dordrecht

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Lam, E., Baltimore, B., Ortiz, W., Melis, A., Chain, R.K., Malkin, R. (1984). Structure and Functional Studies of an Oxygen-evolving Photosystem II Preparation. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_47

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  • DOI: https://doi.org/10.1007/978-94-017-4973-2_47

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-247-2944-9

  • Online ISBN: 978-94-017-4973-2

  • eBook Packages: Springer Book Archive

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