Skip to main content
SpringerLink
Log in
Book cover

Plant Molecular Biology Manual pp 1–55Cite as

Custom polymerase chain reaction engineering plant expression vectors and genes for plant expression

  • Chapter

Abstract

In Chapter C3 we have shown how the polymerase chain reaction (PCR) amplifi-cation can be an extremely useful technique for the identification of transgenic plants. In this chapter we continue to demonstrate the vast capabilities of the PCR technique by showing that it can be used to aid in the construction of plant expression vectors and the engineering of beneficial genes for expression in these vectors. The engineered expression vectors and genes described in this chapter have the potential to produce superior plant varieties that are protected from plant virus infections (Sections 1 and 2) or produce seed proteins with improved nutritional quality (Sections 3 to 5).

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allison R, Johnston RE, Dougherty WG (1986) The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9–20.

    Article  PubMed  CAS  Google Scholar 

  2. Altenbach SB, Pearson KW, Leung FW, Sun SSM (1987) Cloning and sequence analysis of a cDNA encoding a Brazil nut protein exceptionally rich in methionine. Plant Mol Biol 8: 239–250.

    Article  CAS  Google Scholar 

  3. Altenbach SB, Pearson KW, Meeker G, Saraci LC, Sun SSM (1989) Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants. Plant Mol Biol 13: 513–522.

    Article  PubMed  CAS  Google Scholar 

  4. An G (1986) Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells. Plant Physiol 81: 86–91.

    Article  PubMed  CAS  Google Scholar 

  5. Beaucage SL, Caruthers MH (1981) Deoxynucleoside phosphoramidites–A new class of key intermediates for deoxypolynucleotide synthesis. Tetrahedron Lett 22: 1859–1862.

    Article  CAS  Google Scholar 

  6. Benfey PN, Chua N-H (1989) Regulated genes in transgenic plants. Science 244: 174–181.

    Article  PubMed  CAS  Google Scholar 

  7. Benfey PN, Ren L, Chua N-H (1989) The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J 8: 2195–2202.

    CAS  Google Scholar 

  8. Carrington JC, Dougherty WG (1987) Processing of the tobacco etch virus 49 K protease requires autoproteolysis. Virology 160: 355–362.

    Article  PubMed  CAS  Google Scholar 

  9. Carrington JC, Cary SM, Parks TD, Dougherty WG (1989) A second proteinase encoded by a plant potyvirus genome. EMBO J 8: 365–370.

    CAS  Google Scholar 

  10. Chee PP, Klassy RC, Slightom JL (1986) Expression of a bean storage protein `phaseolin minigene’ in foreign plant tissues. Gene 41: 47–57.

    Article  PubMed  CAS  Google Scholar 

  11. Chen Z-L, Schuler MA, Beachy R (1986) Functional analysis of regulatory elements in a plant embryo-specific gene. Proc Natl Acad Sci USA 83: 8560–8564.

    Article  PubMed  CAS  Google Scholar 

  12. Domier LL, Franklin KM, Shahabuddin M, Hellmann GM, Overmeyer JH, Hiremath ST, Siaw MFE, Lomonossoff GP, Shaw JG, Rhoads RE (1986) The nucleotide sequence of tobacco vein mottling virus RNA. Nucl Acids Res 14: 5417–5430.

    Article  PubMed  CAS  Google Scholar 

  13. Dougherty WG, Carrington JC (1988) Expression and function of potyviral gene products. Ann Rev Phytopath 26: 123–143.

    Article  CAS  Google Scholar 

  14. Dougherty WG, Cary SM, Parks TD (1989) Molecular genetic analysis of a plant virus polypro-tein cleavage site: a model. Virology 171: 356–364.

    Article  PubMed  CAS  Google Scholar 

  15. Doyle JJ, Schuler MA, Godette WD, Zenger V, Beachy RN, Slightom JL (1986) The glyco-sylated seed storage proteins of Glycine max and Phaseolus vulgaris: structural homologies of genes and proteins. J Biol Chem 261: 9228–9238.

    PubMed  CAS  Google Scholar 

  16. Erlich HA (1989) PCR technology: Principles and Applications for DNA Amplification. Stockton Press, New York.

    Google Scholar 

  17. Gallie DR, Sleat DE, Watts JW, Turner PC, Wilson TMA (1987) A comparison of eukaryotic viral 5’-leader sequences as enhancers of mRNA expression in vivo. Nucl Acids Res 15: 8693–8711.

    Article  PubMed  CAS  Google Scholar 

  18. Guilley H, Dudley RK, Jonard G, Balazs E, Richards KE (1982) Transcription of cauliflower mosaic virus DNA: detection of promoter sequences, and characterization of transcripts. Cell 30: 763–773.

    Article  PubMed  CAS  Google Scholar 

  19. Hatzenbuhler NT, Rehberg EF, Carter JB, Palermo DP, Hubert EV, Bergum PW, Erickson LA, Marotti KR (1989) Design and chemoenzymatic synthesis of a tissue plasminogen activator gene with unique restriction sites: a model for studying protein domain function. Nucleosides Nucleo-tides 8: 991–994.

    Article  Google Scholar 

  20. Hepburn AG, White J, Pearson L, Maunders MJ, Clarke LE, Prescott AG, Blundy KS (1985) The use of pNJ5000 as an intermediate vector for the genetic manipulation of Agrobacterium Ti-plasmids. J Gen Microbiol 131: 2961–2969.

    PubMed  CAS  Google Scholar 

  21. Innis MA, Gelfand DH, Sninsky JJ, White TJ (1989) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York.

    Google Scholar 

  22. Jobling SA, Gehrke L (1987) Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence. Nature 325: 622–625.

    Article  PubMed  CAS  Google Scholar 

  23. Kaufman DL, Evans GA (1990) Restriction endonuclease cleavage at the termini of PCR products. Biotechniques 9: 304–306.

    PubMed  CAS  Google Scholar 

  24. Kay R, McPherson J (1987) Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucl Acids Res 15: 2778.

    Article  PubMed  CAS  Google Scholar 

  25. Keohavong P, Thilly WG (1989) Fidelity of DNA polymerases in DNA amplification. Proc Natl Acad Sci USA 86: 9253–9257.

    Article  PubMed  CAS  Google Scholar 

  26. Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44: 283–292.

    Article  PubMed  CAS  Google Scholar 

  27. Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA (1987) Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48.

    CAS  Google Scholar 

  28. MacFerrin KD, Terranoma MP, Schreiber SL, Verdine GL (1990) Overproduction and dis-section of proteins by the expression-cassette polymerase chain reaction. Proc Natl Acad Sci USA 87: 1937–1941.

    Article  PubMed  CAS  Google Scholar 

  29. Maiss E, Timpe U, Briske A, Jelkmann W, Casper R, Himmler G, Mattanovich D, Katinger HWD (1989) The complete nucleotide sequence of plum pox virus RNA. J Gen Virology 70: 513–524.

    Article  CAS  Google Scholar 

  30. Maniatis T, Fritsch E, Sambrook J (1982)Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor NY.

    Google Scholar 

  31. Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Meth Enzymol 65: 499–560.

    Article  PubMed  CAS  Google Scholar 

  32. Morelle G (1989) A plasmid extraction procedure on a miniprep scale. Focus 11: 7–8.

    Google Scholar 

  33. Mullis KB, Faloona F, Scharf SJ, Saiki RK, Horn GT, Erlich HA (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp Quant Biol 51: 263–273.

    Article  PubMed  CAS  Google Scholar 

  34. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 4321–4325.

    Article  PubMed  CAS  Google Scholar 

  35. Namba S, Ling K-S, Gonsalves C, Slightom JL, Gonsalves D (1991) Transgenic plants that express watermelon mosaic virus II, zucchini yellow mosaic virus, or papaya ringspot virus coat protein gene show different level of protection when challenge inoculated with various potyvi-ruses (in preparation).

    Google Scholar 

  36. Ow DW, Jacobs JD, Howell SH (1987) Functional regions of the cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity. Proc Natl Acad Sci USA 84: 4870–4874.

    Article  PubMed  CAS  Google Scholar 

  37. Pietrzak M, Shillito RD, Hohn T, Potrykus I (1986) Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector. Nucl Acids Res 14: 5857–5868.

    Article  PubMed  CAS  Google Scholar 

  38. Quemada H, Kearney C, Gonsalves D, Slightom JL (1989) Nucleotide sequences of the coat protein genes and flanking regions of cucumber mosaic virus strains C and WL RNA 3. J Gen Virol 70: 1065–1073.

    Article  PubMed  CAS  Google Scholar 

  39. Quemada H, L’Hostis B, Gonsalves D, Reardon I, Heinrickson R, Hiebert EL, Sieu LC, Slightom JL (1990) The nucleotide sequences of the 3’ terminal regions of papaya ringspot virus strains W and P. J Gen Virol 71: 203–210.

    Article  PubMed  CAS  Google Scholar 

  40. Quemada H, Sieu LC, Siemieniak DR, Gonsalves D, Slightom JL (1990) Watermelon mosaic virus II and Zucchini yellow mosaic virus: cloning of 3’ -terminal regions, nucleotide sequences, and phylogenic comparisons. J Gen Virol 71: 1451–1460.

    Article  PubMed  CAS  Google Scholar 

  41. Quemada H, Gonsalves D, Slightom JL (1991) Expression of cucumber mosaic virus strain-C coat protein gene: analysis of protection against infections by CMV strains -C, -WL, or -Chi using mechanical and aphid transmission vectors. Phytopathology, in press.

    Google Scholar 

  42. Rehberg EF, Theriault NY, Carter JB, Palermo DP, Hubert EV, Bergum PW, Wunderlich CJ, Erickson LA, Marotti KR (1989) Construction of a tissue plasminogen activator gene having unique restriction sites to facilitate domain manipulation. Protein Enging 2: 371–377.

    Article  CAS  Google Scholar 

  43. Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F (1989) Nucleotide sequence of potato virus Y ( N strain) genomic RNA. J Gen Virol 70: 935–947.

    Google Scholar 

  44. Rogers SO, Bendich A (1988) Extraction of DNA from plant tissues. In: Gelvin SB et al. (eds), Plant Molecular Biology Manual, Chap. A6. Kluwer Academic Publishers, Dordrecht, Netherlands.

    Google Scholar 

  45. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics Proc Natl Acad Sci USA 81: 8014–8018.

    CAS  Google Scholar 

  46. Saiki RK, Gelfand DH, Stoffel S, Scharf S, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491.

    Article  PubMed  CAS  Google Scholar 

  47. Scharf SJ, Horn GT, Erlich HA (1986) Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233: 1076–1078.

    Article  PubMed  CAS  Google Scholar 

  48. Siemieniak DR, Sieu LC, Slightom JL (1991) Strategy and methods for directly sequencing cosmid clones. Anal Biochem 192: 441–448.

    Article  PubMed  CAS  Google Scholar 

  49. Sinha ND, Biernat J, McManus J, Koster H (1984) Polymer support oligonucleotide synthesis XVIII: use of f3cyanoethyl-N, N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product. Nucl Acids Res 12: 4539–4557.

    Google Scholar 

  50. Slightom JL, Sun SM, Hall TC (1983) Complete nucleotide sequence of a French bean storage protein gene: plaseolin. Proc Natl Acad Sci USA 80: 1897–1901.

    Article  PubMed  CAS  Google Scholar 

  51. Slightom JL, Drong RF, Klassy RC, Hoffman LM (1985) Nucleotide sequences from phaseolin cDNA clones: the major storage proteins form Phaseolus vulgaris are encoded by two unique gene families. Nucl Acids Res 13: 6483–6498.

    Article  PubMed  CAS  Google Scholar 

  52. Slightom JL, Siemieniak DR, Sieu LC (1991) DNA sequencing: strategy and methods to directly sequence large DNA molecules. In: Miyamoto MM, Cracraft J (eds), Phylogenetic Analysis of DNA. Oxford University Press, Oxford, in press.

    Google Scholar 

  53. Slightom JL (1991) Custom polymerase-chain-reaction engineering of a plant expression vector. Gene 100, in press.

    Google Scholar 

  54. Tindall KR, Kunkel TA (1988) Fidelity of DNA synthesis by Thermus aquaticus DNA polymerase. Biochemistry 27: 6008–6013.

    Article  PubMed  CAS  Google Scholar 

  55. Yeh S-D, Gonsalves D (1985) Translation of papaya ringspot virus RNA in vitro: detection of a possible polyprotein that is processed for capsid protein, cylindrical-inclusion protein, and amorphous-inclusion protein. Virology 143: 260–271.

    Article  PubMed  CAS  Google Scholar 

  56. Yu MH, Frenkel MJ, McKern NM, Shukla DD, Strike PM, Ward CW (1989) Coat protein of potyviruses. 6. Amino acid sequence suggest watermelon mosaic virus 2 and soybean mosaic virus-N are strains of the same potyvirus. Arch Virol 105: 55–64.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Biology—Unit 7242, The Upjohn Company, Kalamazoo, MI, 49007, USA

    Jerry L. Slightom, Roger F. Drong, Leang C. Sieu & Paula P. Chee

Authors
  1. Jerry L. Slightom
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Roger F. Drong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Leang C. Sieu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paula P. Chee
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Purdue University, West Lafayette, Indiana, USA

    Stanton B. Gelvin

  2. Leiden State University, Leiden, The Netherlands

    Robbert A. Schilperoort

  3. Ohio State University, Columbus, Ohio, USA

    Desh Pal S. Verma

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media New York

About this chapter

Cite this chapter

Slightom, J.L., Drong, R.F., Sieu, L.C., Chee, P.P. (1991). Custom polymerase chain reaction engineering plant expression vectors and genes for plant expression. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4820-9_1

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-4820-9_1

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-1341-0

  • Online ISBN: 978-94-017-4820-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation