Abstract
When forty years ago, I made my first attempt at preparing isolated hepatocytes it would have been quite impossible to imagine the extraordinary diversity of studies for which they would be employed. Indeed, many of the topics covered in this book were not even part of the body of scientific knowledge at that time, and it is pleasing to know that much new knowledge concerning the liver has come to light as a consequence of the availability of the isolated hepatocyte preparation. In this chapter I will give a brief history of the development of methods for isolated hepatocyte preparation. In Chapter 21 the results of some of the metabolic studies of our group are described in the context of macromolecular organisation.
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References
Berry MN. Metabolic properties of cells isolated from adult mouse liver. J Cell Biol 1962; 15: 1–8.
Anderson NG. Mass isolation of whole cells from rat liver. Science 1953; 117: 627–628.
Berry MN and Simpson FO. Fine structure of cells isolated from adult mouse liver. J Cell Biol 1962; 15: 9–17.
Rodbell M. Metabolism of isolated fat cells. J Biol Chem 1964; 239: 375–380.
Howard RB, Christensen AK, Gibbs FA and Pesch LA. The enzymatic preparation of isolated intact parenchymal cells from rat liver. J Cell Biol 1967; 35: 675–684.
Berry MN and Friend DS. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol 1969; 43: 506–520.
Berry MN, Werner HV and Kun E. Effects of bicarbonate on intercompartmental reducing-equivalent translocation in isolated parenchymal cells from rat liver. Biochem J 1974; 140: 355–361.
Seglen PO. Preparation of rat liver cells. I. Effect of Cat+ on enzymatic dispersion of isolated, perfused liver. Exp Cell Res 1972; 74: 450–454.
Dawson RMC, Elliott DC, Elliott WH and Jones K.M. Data for Biochemical Research. 3rd ed. Oxford: Oxford University Press, 1986.
Berry MN, Edwards AM and Barritt GJ. Isolated Hepatocytes Preparation, Properties and Application. Amsterdam: Elsevier, 1991.
Ferzandi TR and MacGregor RR. Thapsigargin shifts the Ca set point of parathyroid cells to lower extracellular [Ca]. Endocrine 1997; 7: 361–366.
Bissell DM, Hammaker LE and Meyer UA. Parenchymal cells from adult rat liver in nonproliferating monolayer culture. I. Functional studies. J Cell Biol 1973; 59: 722–734.
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© 2000 Springer Science+Business Media Dordrecht
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Berry, M.N. (2000). Isolated hepatocytes: forty years on. In: Berry, M.N., Edwards, A.M. (eds) The Hepatocyte Review. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3345-8_1
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DOI: https://doi.org/10.1007/978-94-017-3345-8_1
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5402-9
Online ISBN: 978-94-017-3345-8
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