Abstract
Since AMF are obligate biotrophs, their culture in in vitro condition is rather difficult and has imposed a major limitation for its practical exploitation and understanding the physiological, biochemical and genetical characteristics of the organism. The common inoculum production methods involving soil-root culture, inoculum rich soil-pellets, soil-free inoculum, nutrient film culture and aeroponic culture systems also do not fulfill the actual need of the inoculum. Recently the use of Ri T-DNA transformed roots (hairy roots) has given a new impetus to the culture production of these obligate symbionts. The procedures and technologies developed so far are described and their usefulness is discussed in detail.
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Abdul-Khaliq, Gupta, M.L., Alam, M. (2002). Biotechnological Approaches for Mass Production of Arbuscular Mycorrhizal Fungi : Current Scenario and Future Strategies. In: Mukerji, K.G., Manoharachary, C., Chamola, B.P. (eds) Techniques in Mycorrhizal Studies. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3209-3_16
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DOI: https://doi.org/10.1007/978-94-017-3209-3_16
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