Abstract
Somatic embyrogenesis is a promising tool in micropropagation. It is, however, a fact that the poor quality of produced somatic embryos restrict their commercial application. Somatic embryogenic suspension cultures contain embryos at various developmental stages, various shaped embryos, and even non-embryogenic tissues. As a result, the suspension cultures show heterogeneity in embryo quality, thus hindering the development of transplant production systems using somatic embyrogenesis. Methods which evaluate and sort somatic embryos by information related to embryo quality show good potential for obtaining uniform and high quality embryos. It is desirable to have an embryo quality evaluation method which is simple, nondestructive, and has potential for automation. Image analysis techniques, which extract features of an object from visual data, fulfill the above requirements and appear to be promising for use in developing embryo evaluation and sorting systems. Based on this idea, many applications of image analysis have been reported for the evaluation of somatic embryos. In this chapter, by introducing image analysis applications to somatic embyrogenesis and to embryo sorting systems, the problems and feasibility of somatic embryo sorting using image analysis will be discussed.
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Ibaraki, Y. (1999). Image Analysis for Sorting Somatic Embryos. In: Jain, S.M., Gupta, P.K., Newton, R.J. (eds) Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 55. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3032-7_7
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DOI: https://doi.org/10.1007/978-94-017-3032-7_7
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