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Plant Cell and Tissue Culture pp 475–496Cite as

In vitro Culture of Tropical Fruits

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Abstract

Contributions to tropical fruit improvement by conventional plant breeding have been limited by complex genetic systems, extended juvenility, lack of knowledge, and in many cases, lack of effort. Most of today’s important tropical fruit cultivars arose from the selection of mutations within existing clones, or by selections from seedling populations derived from open pollinations. Due to the increasing significance of tropical fruits for food and export income, there has been a recent expansion of effort and resources devoted to tropical fruit cultivar improvement. Many programs are attempting to integrate emerging biotechnologies with conventional breeding methods to facilitate and expedite cultivar improvement. The objective of this chapter is to provide some pertinent information regarding in vitro methods for tropical fruit improvement, along with a discussion of how such methods can be applied to the genetic improvement of tropical fruits, with emphasis on increasing fruit quality and resistance to disease and environmental stress.

Keywords

  • Somatic Embryo
  • Somatic Embryogenesis
  • Plant Regeneration
  • Embryogenic Callus
  • Somatic Hybrid

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Amin, M.N. and Jaiswal, V.S. (1987). Rapid clonal propagation of guava through in vitro shoot proliferation and nodal explants of mature trees. Plant Cell Tissue Org. Cult. 9: 235–243.

    Google Scholar 

  • Arrias, O. and Huete, F. (1983). Propagation vegetativa in vitro de pejibaye (Bactris gasipaes H.B.K.). Turrialba 33: 103–108.

    Google Scholar 

  • Barbieri, C. and Morini, S. (1987). Plant regeneration from Actinidia callus cultures. J. Am. Soc. Hort. Sci. 62: 107–109.

    Google Scholar 

  • Barlass, M. and Skene, K.G.M. (1982). In vitro plantlet formation from Citrus species and hybrids. Sci. Hort. 17:333–341.

    Google Scholar 

  • Beachy, R.N., Rogers, S.G. and Fraley, R.T. (1987). Genetic transformation to confer resistance to plant virus disease. Genetic Eng. 9: 229–247.

    CrossRef  CAS  Google Scholar 

  • Ben-Hayyim, G. and Neumann, H. (1983). Stimulatory effect of glycerol on growth and somatic embryogenesis in citrus callus cultures. Z. Pflanzenphysiol. 110: 331–338.

    CAS  Google Scholar 

  • Berg, L.A. and Bustamante, M. (1974). Heat treatment and meristem culture for the production of virus free bananas. Phytopathology 64: 320–322.

    CrossRef  Google Scholar 

  • Bouzid, S. (1975). Quelques traits du comportement de boutures de Citrus en culture in vitro. Comptes Rendus de l’Academie des Sciences Serie D 280: 1689–1692.

    Google Scholar 

  • Chaturvedi, H.C. and Mitra, G.C. (1974). Clonal propagation of Citrus from somatic callus cultures. Hort. Sc. 9: 188–120.

    Google Scholar 

  • Chen, Z. (1990). Citrus: Anther Culture, Ch. 25. In: Handbook of Plant Cell Culture, Vol. 6. Z. Chen, D.A. Evans, W.R. Sharp, P.V. Ammirato and M.R. Sondahl (eds.), Perennial Crops. McGraw-Hill, New York, pp. 365–375.

    Google Scholar 

  • Deng, X.X., Grosser, J.W. and Gmitter, F.G., Jr. (1992). Intergeneric somatic hybrid plants from protoplast fusion of Fortunella crassifolia `Meiwa’ with Citrus sinensis `Valencia’. Sci. Hort. 49: 55–62.

    CrossRef  Google Scholar 

  • DeWald, S.G., Litz, R.E. and Moore, G.A. (1989a). Optimizing somatic embryo production in mango. J. Am. Soc. Hort. Sci. 114: 712–716.

    Google Scholar 

  • DeWald, S.G., Litz, R.E. and Moore, G.A. (1989b). Maturation and germination of mango somatic embryos. J. Am. Soc. Hort. Sci. 114: 837–841.

    Google Scholar 

  • Fahn, A. (1974). Plant Anatomy. Pergamon Press, New York.

    Google Scholar 

  • Fitch, M.M., Manshardt, R.M., Gonsalves, D., Slighton, J.L. and Sanford, J.C. (1990). Stable transformation of papaya via microprojectile bombardment. Plant Cell Rep. 9: 189–194.

    CAS  Google Scholar 

  • Fu, L. (1990). Litchi: Anther Culture, Ch. 16. In: Handbook of Plant Cell Culture. Vol. 6. Z. Chen, D.A. Evans, W.R. Sharp, P.V. Ammirato and M.R. Sondahl (eds.), Perennial Crops. McGraw-Hill, New York, pp. 376–384.

    Google Scholar 

  • Gmitter, F.G., Jr. and Ling, X. (1991). Embryogenesis in vitro and nonchimeric tetraploid plant recovery from undeveloped Citrus ovules treated with colchicine. J. Am. Soc. Hort. Sci. 116: 317–321.

    Google Scholar 

  • Gmitter, F.G., Jr., Ling, X.B. and Deng, X.X. (1990). Induction of triploid Citrus plants from endosperm calli in vitro. Theor. Appl. Genet. 80: 785–790.

    CrossRef  Google Scholar 

  • Gmitter, F.G., Jr., Ling, X., Cai, C. and Grosser, J.W. (1991). Colchicine-induced polyploidy in Citrus embryogenic cultures, somatic embryos, and regenerated plantlets. Plant Sci. 74: 135–141.

    CrossRef  CAS  Google Scholar 

  • Gmitter, F.G., Jr. and Moore, G.A. (1986). Plant regeneration from undeveloped ovules and embryogenic calli of Citrus: Embryo production, germination, and plant survival. Plant Cell Tissue Org. Cult. 6: 139–147.

    CAS  Google Scholar 

  • Goh, H.K.L., Rao, A.N. and Loh, C.S. (1988). In vitro plantlet formation in mangosteen (Garcinia mangostana L.). Ann. Bot. 62:87–93.

    Google Scholar 

  • Grinblat, H. (1972). Differentiation of Citrus stem in vitro. J. Am. Soc. Hort. Sci. 97:599–603. Grosser, J.W. (1990). Citrus rootstock improvement by cell fusion. Citrus and Veg. Mag., September, pp. 28, 31–32.

    Google Scholar 

  • Grosser, J.W. and Chandler, J.L. (1986). In vitro multiplication of Swingle citrumelo rootstock with coumarin. Hort. Sc. 21:518–520.

    Google Scholar 

  • Grosser, J.W. and Chandler, J.L. (1987). Aseptic isolation of leaf protoplasts from Citrus, Poncirus, Citrus x Poncirus hybrids and Severinia for use in somatic hybridization experiments. Sci. Hort. 31: 253–257.

    CrossRef  Google Scholar 

  • Grosser, J.W. and Gmitter F.G., Jr. (1990a). Protoplast fusion in Citrus improvement. Plant Breed. Rev. 8: 339–374.

    Google Scholar 

  • Grosser, J.W. and Gmitter, F.G., Jr. (19906). Wide hybridization of Citrus via protoplast fusion: Progress, strategies and limitations. In: Horticultural Biotechnology, Wiley-Liss, New York, pp. 31–41.

    Google Scholar 

  • Grosser, J.W. and Gmitter, F.G., Jr. (1990c). Somatic hybridization of Citrus with wild relatives for germplasm enhancement and cultivar development. Hort. Sc. 25: 147–151.

    Google Scholar 

  • Grosser, J.W., Gmitter, F.G., Jr. and Chandler, J.L. (1988a). Intergeneric somatic hybrid plants of Citrus sinensis cv. Hamlin and Poncirus trifoliata cv. Flying Dragon. Plant Cell Rep. 7: 5–8.

    CrossRef  Google Scholar 

  • Grosser, J.W., Gmitter, F.G., Jr. and Chandler, J.L. (1988b). Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha. Theor. Appl. Genet. 75: 397–401.

    CrossRef  Google Scholar 

  • Grosser, J.W., Gmitter, F.G., Jr., Tusa, N. and Chandler, J.L. (1990). Somatic hybrid plants from sexually incompatible woody species: Citrus reticulata and Citropsis gilletiana. Plant Cell Rep. 8: 656–659.

    CrossRef  Google Scholar 

  • Grosser, J.W., Moore, G.A. and Gmitter, F.G., Jr. (1989). Interspecific somatic hybrid plants from the fusion of `Key’ lime (Citrus aurantifolia) with `Valencia’ sweet orange (Citrus sinensis) protoplasts. Sci. Hort. 39: 23–29.

    CrossRef  Google Scholar 

  • Grumet, R. (1990). Genetically engineered plant virus resistance. Hort. Sc. 25: 508–513.

    CAS  Google Scholar 

  • Gupta, P.P. (1986). Eradication of mosaic disease and rapid clonal multiplication of bananas and plantains through meristem tip culture. Plant Cell Tissue Org. Cult. 6: 33–39.

    CAS  Google Scholar 

  • Gupta, P.K., Kenkurkar, S.V., Kulkarni, V M., Shirgurkar, M.V. and Mascarenhas, A.F. (1984). Somatic embryogenesis and plants from zygotic embryos of coconut (Cocos nucifera L.) in vitro. Plant Cell Rep. 3: 222–225.

    CrossRef  Google Scholar 

  • Hendrix, R.C., Litz, R.E. and Kirchoff, B.G. (1987). In vitro organogenesis and plant regeneration from leaves of Solanum candidum Lindl., S. quitoense Lam. (naranjilla) and S. sessiflorum Dunal. Plant Cell Tissue Org. Cult. 11:67–73.

    Google Scholar 

  • Hidaka, T. and Kajiura, I. (1988) Plantlet differentiation from callus protoplasts induced from Citrus embryo. Sci. Hort. 34: 85–92.

    CrossRef  Google Scholar 

  • Hidaka, T., Omura, M., Ugaki, M., Tomiyama, M., Kato, A., Oshima, M. and Motoyoshi, F. (1990). Agrobacterium-mediated transformation and regeneration of Citrus spp. from suspension cells. Japan. J. Breed. 4: 199–207.

    Google Scholar 

  • Jaiswal, V.S. and Narayan, P. (1985). Regeneration of plantlets from the callus of stem segments of adult plants of Ficus religiosa L. Plant Cell Rep. 4: 256–258.

    CrossRef  CAS  Google Scholar 

  • Jordan, M. (1988). Multiple shoot formation and rhizogenesis from cherimola (Annona cheri- mola L.) hypocotyls and petiole explants. Gartenbauwissenschaften 53: 234–237.

    Google Scholar 

  • Karunaratne, S. and Periyapperuma, K. (1989). Culture of immature embryos of coconut, Cocos nucifera L., callus proliferation and somatic embryos. Plant Sci. 62: 247–253.

    CrossRef  Google Scholar 

  • Kobayashi, S. and Ohgawara, T. (1988). Production of somatic hybrid plants through protoplast fusion in Citrus. J. Agric. Rev. Quarterly 22: 181–188.

    Google Scholar 

  • Kobayashi, S., Ohgawara, T., Ohgawara, E., Oiyama, I. and Ishii, S. (1988). A somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin. Plant Cell Tissue Org. Cult. 14: 63–69.

    Google Scholar 

  • Kobayashi, S., Oiyama, I., Yoshinaga, K., Ohgawara, T., and Ishii, S. (1991). Fertility in an intergeneric somatic hybrid plant of Rutaceae. Hort. Sc. 26: 207.

    Google Scholar 

  • Kobayashi, S. and Uchimaya, H. (1989). Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer. Japan. J. Genet. 64: 91–97.

    CrossRef  Google Scholar 

  • Kobayashi, S., Uchimaya, H. and Ikeda, I. (1983) Plant regeneration from `Trovita’ orange protoplasts. Japan. J. Breed. 33: 119–122.

    Google Scholar 

  • Kochba, J., Spiegel-Roy, P., Neumann, H. and Saad, S. (1982). Effect of carbohydrates on somatic embryogenesis in subcultured nucellar callus of Citrus cultivars. Z. Pflanzenphysiol. 105:359–368.

    Google Scholar 

  • Kochba, J., Spiegel-Roy, P. and Safran, H. (1972). Adventive plants from ovules and nucelli in citrus. Planta 106: 237–245.

    CrossRef  Google Scholar 

  • Krug, C.A. and Bacchi, O. (1943). Triploid varieties of Citrus. J. Heredity 34: 277–283.

    Google Scholar 

  • Litz, R.E. (1984a). In vitro somatic embryogenesis from callus of jaboticaba, Myrciaria cauliflora. Hort. Sc. 190:62–64.

    Google Scholar 

  • Litz, R.E. (1984b). In vitro responses of adventitious embryos of two polyembryonic Eugenia species. Hort. Sc. 19:720–722.

    Google Scholar 

  • Litz, R.E. (1985). Somatic embryogenesis in tropical fruit trees. In: Tissue Culture in Forestry and Agriculture. R.R. Henke, K.W. Hughes, M.J. Constantin and A. Hollaender (eds.), Plenum, New York, pp. 179–193.

    Google Scholar 

  • Litz, R.E. (1987). Application of tissue culture to tropical fruits. In: Plant Tissue and Cell Culture. C.E. Green, D.A. Somers, W.A. Hackett and D.D. Biesboer (eds.), Alan R. Liss, New York, pp. 407–418.

    Google Scholar 

  • Litz, R.E. (1988). Somatic embryogenesis from cultured leaf expiants of the tropical tree Euphoria longan Stend. J. Plant Physiol. 132: 190–193.

    CrossRef  CAS  Google Scholar 

  • Litz, R.E. and Conover, R.A. (1980). Partial organogenesis in tissue cultures of Averrhoa carambola. Hort. Sc. 15: 735.

    Google Scholar 

  • Litz, R.E. and Conover, R.A. (1982). In vitro somatic embryogenesis and plant regeneration from Carica papaya L. ovular callus. Plant Sci. Lett. 26:153–158.

    Google Scholar 

  • Litz, R.E. and Conover, R.A. (1983). High frequency somatic embryogenesis from Carica suspension cultures. Ann. Bot. 51: 683–686.

    Google Scholar 

  • Litz, R.E. and Griffis, J.L. (1989). Carambola (Averrhoa carambola L.). In: Biotechnology in Agriculture and Forestry, Vol. 5. Y.P.S. Bajaj (ed.), Trees II. Springer Verlag, Berlin, pp. 59–67.

    Google Scholar 

  • Litz, R.E. and Gray, D.J. (1992). Organogenesis and somatic embryognesis. In: Biotechnology of Perennial Fruit Crops, Chapter 1. F. Hammerschlag and R. Litz (eds.), pp. 3–34. CAB International, Wallingford, Oxon, U.K.

    Google Scholar 

  • Litz, R.E. and Jaiswal, V.S. (1991). Micropropagation of tropical and subtropical fruits. In: Micropropagation. P.C. Debergh and R.H. Zimmerman (eds.), Kluwer Academic Publishers, Dordrecht, pp. 247–263.

    CrossRef  Google Scholar 

  • Litz, R.E., Knight, R.J. and Gazit, S. (1984). In vitro somatic embryogenesis from Mangifera indica L. callus. Sci. Hort. 22:233–240.

    Google Scholar 

  • Litz, R.E., Moore, G.A. and Srinivasan, C. (1985). In vitro systems for propagation and improvement of tropical fruits and palms. Hort. Rev. 7:157–200.

    Google Scholar 

  • Litz, R.E., O’Hair, S. K. and Conover, R.A. (1983). In vitro growth of Carica papaya L. cotyledons. Sci. Hort. 19:287–293.

    Google Scholar 

  • Ma, S.S. and Shii, C.T. (1974). Growing banana plantlets from adventitious buds. J. Chinese Soc. Hort. Sci. 20: 6–12 (in Chinese).

    Google Scholar 

  • Maheshwari, P. and Rangaswamy, N.S. (1958). Polyembryony and in vitro cultures of embryos of Citrus and Mangifera. Indian J. Hort. 15: 272–282.

    Google Scholar 

  • Mapes, M.O. (1974). Tissue culture of Bromeliada. Int. Plant Prop. Comb. Soc. 23: 47–55.

    Google Scholar 

  • Menczel, L., Nagy, F., Kiss, Z. and Maliga, P. (1981). Streptomycin resistant and sensitive hybrids of Nicotiana tabacum and Nicotiana knightiana: Correlation of resistance with N. tabacum plastids. Theor. Appl. Genet. 59: 191–195.

    CrossRef  CAS  Google Scholar 

  • Mitra, G.C. and Chaturvedi, H.C. (1972). Embryoids and complete plants from unpollinated ovaries and from ovules of in vitro-grown emasculated flower buds of Citrus spp. Bull. Torrey Bot. Club 99: 184–189.

    CrossRef  Google Scholar 

  • Moore, G.A. (1985). Factors affecting in vitro embryogenesis from undeveloped ovules of mature Citrus fruit. J. Am. Soc. Hort. Sci. 110: 60–70.

    Google Scholar 

  • Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    CrossRef  CAS  Google Scholar 

  • Murashige, T. and Tucker, D.P.H. (1969). Growth factor requirements of citrus tissue culture. Proc. First Int. Citrus Symp. 3: 1155–1161.

    CAS  Google Scholar 

  • Nair, S., Gupta, P. K., Shirgurkas, M. V. and Mascarenhas, A. F. (1984). In vitro organogenesis from leaf explants of Annona squamosa Linn Plant Cell Tissue Org. Cult. 3:29–40.

    Google Scholar 

  • Navarro, L., Jaurez, J., Pina, J.A., Ballester, J.F. and Arregui, J.M. (1988). The citrus variety improvement program in Spain after eleven years. In: L.W. Timmer, S.M. Garnsey and L. Navarro (eds.), Proceedings 10th Conf. Intl. Organ. Cirus Virol, pp. 400–406.

    Google Scholar 

  • Navarro, L., Ortiz, J.M. and Juarez, J. (1985). Aberrant citrus plants obtained by somatic embryogenesis of nucelli cultured in vitro. Hort.Sc. 20: 214–215.

    Google Scholar 

  • Navarro, L., Roistacher, C.N. and Murashige, T. (1975). Improvement of shoot-tip grafting in vitro for virus-free citrus. J. Am. Soc. Hort. 100: 471–479.

    Google Scholar 

  • Novak, F.J., Afza, R., Van Duren, M., Perea Dallos, M., Conger, B.V. and Xiaolang, T. (1989). Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking (ABB) bananas (Musa spp.). Biotechnology 7: 154–159.

    CrossRef  Google Scholar 

  • Ohgawara, T., Kobayashi, S., Ishii, S., Yoshinaga, K. and Oiyama, I. (1989). Somatic hybridization in Citrus: Navel orange (C. sinensis Osb.) and grapefruit (C. paradisi Macf.). Theor. Appl. Genet. 71: 1–4.

    CrossRef  Google Scholar 

  • Ohgawara, T., Kobayashi, S., Ishii, S., Yoshinaga, K. and Oiyama, I. (1991). Fertile fruit trees obtained by somatic hybridization: Navel orange (Citrus sinensis) + Troyer citrange (C. sinensis x Poncirus trifoliata). Theor. Appl. Genet. 81: 141–143.

    CrossRef  Google Scholar 

  • Ohgawara, T., Kobayashi, S., Ohgawara, E., Uchimaya, H. and Ishii, S. (1985). Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor. Appl. Genet. 71: 1–4.

    CrossRef  Google Scholar 

  • Ohyama, K. and Oka, S. (1982). Multiple shoot formation from mulberry (Morus alba L.) hypocotyl by N-(2-chloro-4-pyridyl)-N’-phenylurea. 5th Intl. Cong. Plant Tissue and Cell Culture, Tokyo, pp. 149–150.

    Google Scholar 

  • Oiyama, I., Kobayashi, S., Yoshinaga, K., Ishii, S. and Ohgawara, T. (1991). Use of pollen from a somatic hybrid between Citrus and Poncirus in the production of triploids. Hort. Sc. 26: 1082.

    Google Scholar 

  • Oka, S. and Ohyama, K. (1981). In vitro initiation of adventitious buds and its modification by high concentrations of benzyladenine in leaf tissues of mulberry (Morus alba). Can. J. Bot. 59: 68–74.

    Google Scholar 

  • Oliveira, M.M. and Pais, M.S. (1991). Plant regeneration from protoplasts by long-term calius cultures of Actinidia deliciosa var. deliciosa cv. Hayword (Kiwifruit). Plant Cell Rep. 9; 643–646.

    CrossRef  Google Scholar 

  • Omura, M., Matsuta, N., Moriguchi, T. and Kozaki, I. (1987). Adventitious shoot and plantlet formation from cultured pomegranate leaf explants. Hort. Sc. 22: 133–134.

    CAS  Google Scholar 

  • Pais, M.S., Oliveira, M.M. and Barroso, J. (1987). Use of petiole segments of Actinidia chinensis (Kiwi) for plant differentiation and production of friable calli for protoplast isolation. Acta Hort. 212: 687–690.

    Google Scholar 

  • Pang, S.C. and Sanford, J.C. (1988). Agrobacterium mediated gene transfer in papaya. J. Am. Soc. Hort. Sci. 113: 287–291.

    Google Scholar 

  • Philip, V.J. (1984). In vitro organogenesis and plantlet formation in cashew (Anacardium occidentale L.). Ann. Bot. 54:149–152.

    Google Scholar 

  • Raj Bhansali, R. and Arya, A.C. (1978a). Shoot formation in stem and root callus of Citrus aurantifolia (Christm.) Swingle grown in culture. Current Sci. 47: 775–776.

    Google Scholar 

  • Raj Bhansali, R. and Arya, A.C. (1978b). Differentiation in explants of Citrus paradisi Macf. (grapefruit) grown in culture. Indian J. Expt. Biol. 16: 409–411.

    CAS  Google Scholar 

  • Rangan, T.S., Murashige, T. and Bitters, W.P. (1968). In vitro initiation of nucellar embryos in monoembryonic Citrus. Hort. Sc. 3:226–227.

    Google Scholar 

  • Revilla, M.A. and Power, J.B. (1988). Morphogenetic potential of long-term callus cultures of Actinidia deliciosa. J. Hort. Sci. 63: 541–545.

    Google Scholar 

  • Reynolds, J.F. and Murashige, T. (1979). Asexual embryogenesis in callus cultures of palms. In vitro 15: 383–387.

    CrossRef  Google Scholar 

  • Sabharwal, P.S. (1963). In vitro culture of ovules, nucelli and embryos of Citrus reticulata Blanco var. Nagpuri. In: Plant Tissue and Organ Culture–A Symposium. P. Maheshwari and N.S. Rangaswamy (eds.), University of Delhi, Delhi, pp. 265–274.

    Google Scholar 

  • Sauton, A., Mouras, A. and Lutz, A. (1982). Plant regeneration from citrus root meristems. J. Am. Soc. Hort. Sci. 57: 227–231.

    Google Scholar 

  • Scorza, R. and Janick, J. (1976). Tissue culture in passiflora. Proc. Trop. Region Am. Soc. Hort. Sci. 20: 179–183.

    Google Scholar 

  • Sim, G.E., Loh, C.S. and Goh, C.J. (1988). Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco. Plant Cell Rep. 7: 418–420.

    Google Scholar 

  • Smith, C.J.S., Watson, C.F., Ray, J., Bird, C.R., Morris, P.C., Schuch, W. and Grierson, D. (1988). Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334: 724–726.

    CrossRef  CAS  Google Scholar 

  • Soost, R.K. and Cameron, J.W. (1980). `Oroblanco’, a triploid pummelo-grapefruit hybrid. Hort. Sc. 15:667–669.

    Google Scholar 

  • Soost, R.K. and Cameron, J.W. (1985). `Melogold’, a triploid pummelo-grapefruit hybrid. Hort. Sc. 20:1134–1135.

    Google Scholar 

  • Starantino, A. and Recupero, G.R. (1981). Citrus hybrids obtained in vitro from 2x females and 4x males. Proc. Int. Soc. Citriculture, pp. 31–32.

    Google Scholar 

  • Surga Rivas, J.G. (1988). Obtencion de plantas libres del virus mosaico del pepino por cultivo de apices meristematicos aislados in vitro de dos cultivares de banano. Fitopatologia Venezolana 1: 69–72.

    Google Scholar 

  • Swingle, W.T. and Reece, P.C. (1967). The botany of citrus and its wild relatives. In: The Citrus Industry, Vol. 1. W. Reuther, L.D. Batchelor and H.J. Ueber (eds.), University of California Press, Berkeley, pp. 190–430.

    Google Scholar 

  • Sykes, S.R. (1988). Overview of the family Rutaceae. In: R.R. Walker (ed.), Proceedings of the Breeding Workshop, Citrus Breeding in Australia and the Use of New Breeding Technologies. CSIRO, Melbourne, pp. 93–100.

    Google Scholar 

  • Tao, R., Murayama, H., Moriguchi, K. and Sugiura, A. (1988). Plant regeneration from callus cultures derived from primordial leaves of adult Japanese persimmon. Hort. Sc. 23: 1055–1056.

    Google Scholar 

  • Timmer, L. W. (1988). Blight. In: Compendium of Citrus Diseases. J.O. Whiteside, S.M. Garnsey and L.W. Timmer (eds.), APS Press, St. Paul, pp. 66–67.

    Google Scholar 

  • Tsai, C.K. (1988). Plant regeneration from leaf callus protoplasts of Actinidia chinensis Planch. var. chinensis. Plant Sci. 54: 231–235.

    CrossRef  Google Scholar 

  • Tusa, N., Grosser, J.W. and Gmitter, F.G., Jr. (1990). Plant regeneration of `Valencia’ sweet orange,’Femminello’ lemon, and the interspecific somatic hybrid following protoplast fusion. J. Am. Soc. Hort. Sci. 115: 1043–1046.

    Google Scholar 

  • Vaeck, M., Reynaerts, A., Hofte, H., Jansens, S., DeBeuckeleer, M., Dean, C., Zabeau, M., Van Montagu, M. and Leemans, J. (1987). Transgenic plants protected from insect attack. Nature 328: 33–37.

    CrossRef  CAS  Google Scholar 

  • Vardi, A., Arzee-Gonen, P., Frydman-Shani, A., Bleichman, S. and Galun, E. (1989). Protoplast fusion mediated transfer of organelles from Microcitrus and Citrus and regeneration of novel alloplasmic trees. Theor. Appl. Genet. 78: 741–747.

    CrossRef  Google Scholar 

  • Vardi, A., Bleichman, S. and Aviv, D. (1990). Genetic transformation of citrus protoplasts and regeneration of transgenic plants. Plant Sci. 69: 199–206.

    CrossRef  CAS  Google Scholar 

  • Vardi, A., Breiman, A., and Galun, E. (1987). Citrus cybrids: Production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles. Theor. Appl. Genet. 75: 51–58.

    CrossRef  CAS  Google Scholar 

  • Vardi, A. and Galun, E. (1988). Recent advances in protoplast culture of horticultural crops: Citrus. Sci. Hort. 37: 217–230.

    CrossRef  Google Scholar 

  • Vardi, A., Hutchison, D.J. and Galun, E. (1986). A protoplast to tree system in Microcitrus based on protoplasts derived from a sustained embryogenic callus. Plant Cell Rep. 5: 412–414.

    CrossRef  Google Scholar 

  • Vardi, A., Spiegel-Roy, P. and Galun, E. (1982). Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species. Theor. Appl. Genetics 62: 171–176.

    CrossRef  Google Scholar 

  • Wakasa, K. ( 1979. Variation in plants differentiated from tissue culture of pineapple. Japan. J. Breed. 29: 13–22.

    Google Scholar 

  • Wang, D. and Chang, C.J. (1978). Triploid citrus plantlet from endosperm culture (in Chinese). Sci. Sinica 21: 822–827.

    Google Scholar 

  • Wei, W. (1990). Longan: Anther and embryo culture, Ch. 27. In: Handbook of Plant Cell Culture. Vol. 6. Z. Chen, D.A. Evans, W.R. Sharp, P.V. Ammirato and M.R. Sondahl (eds), Perennial Crops. McGraw-Hill, New York, pp. 385–396.

    Google Scholar 

  • Yie, S. and Liaw, S.I. (1977). Plant regeneration from shoot tips and callus of papaya. In vitro 13: 564–567.

    CrossRef  PubMed  CAS  Google Scholar 

  • Yokoyama, T. and Takeuchi, M. (1976). Organ and plantlet formation from callus in Japanese persimmon (Diospyros kaki). Phytomorphology 26: 273–275.

    Google Scholar 

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Authors
  1. J. W. Grosser
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Editors and Affiliations

  1. Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 1143 Fifield Hall, 32611-0690, Gainesville, FL, USA

    Indra K. Vasil

  2. Plant Physiology Research Group, Department of Biological Sciences, The University of Calgary, 2500 University Drive NW, T2N 1N4, Calgary, Alberta, Canada

    Trevor A. Thorpe

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© 1994 Springer Science+Business Media Dordrecht

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Grosser, J.W. (1994). In vitro Culture of Tropical Fruits. In: Vasil, I.K., Thorpe, T.A. (eds) Plant Cell and Tissue Culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2681-8_19

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  • DOI: https://doi.org/10.1007/978-94-017-2681-8_19

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