Optimization of the expression of a transgene in plants

  • Subhash C. Minocha
Part of the Forestry Sciences book series (FOSC, volume 64)


Transgenic manipulation of plants is now a well-established technology for crop improvement. In 1998, more than 30 million hectares of land were devoted to the cultivation of genetically modified crops (Moffat, 1998). While the numbers are impressive, plantations are presently limited to only a few herbaceous annual crops. However, for tree species, which potentially have the most to gain from genetic manipulation technologies (because long breeding cycles limit their genetic improvement through conventional breeding), relatively little progress has been made with the production of transgenic plants.


Transgenic Plant Transit Peptide Trans Gene Matrix Attachment Region Nature Biotech 
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  1. Abler, M.L. and Green, P.J. (1996) Control of mRNA stability in higher plants, Plant Mol Biol 32, 63–78.PubMedGoogle Scholar
  2. Allen, G.C., Hall Jr., G., Michalowski, S., Newman, W., Spiker, S., Weissinger, A.K., and Thompson, W.F. (1996) High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco, Plant Cell 8, 899–913.PubMedGoogle Scholar
  3. Aoyama, T. and Chua, N.-H. (1997) A glucocorticoid-mediated transcriptional induction system in transgenic plants, Plant J 11, 605–612.PubMedGoogle Scholar
  4. Assaad, F.F., Tucker, K.L., and Signer, E.R. (1993) Epigenetic repeat-induced gene silencing (RIGS) in Arabidopsis, Plant Mol Biol 22, 1067–1085.PubMedGoogle Scholar
  5. Bailey-Serres, J. (1999) Selective translation of cytoplasmic mRNAs in plants, TIPS 4, 142–148.Google Scholar
  6. Bailey-Serres, J. and Gallie, D.R. (eds.) (1998) A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, 183 pp.Google Scholar
  7. Bar-Peled, M., Bassham, D.C., and Raikhel, N.V. (1996) Transport of proteins in eukaryotic cells: more questions ahead, Plant Mol Biol 32, 223–249.PubMedGoogle Scholar
  8. Barkan, A. and Stern, D. (1998) Chloroplast mRNA processing: intron splicing and 3’-end metabolism, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 162–173.Google Scholar
  9. Bastola, D.R. and Minocha, S.C. (1995) Increased putrescine biosynthesis through transfer of mouse ornithine decarboxylase cDNA in carrot promotes somatic embryogenesis, Plant Physiol 109, 63–71.PubMedGoogle Scholar
  10. Baulcombe, D.C. (1996) RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants, Plant Mol Biol 32, 79–88.PubMedGoogle Scholar
  11. Bender, J. (1998) Cytosine methylation of repeated sequences in eukaryotes: the role of DNA pairing, TIBS 23, 252–256.PubMedGoogle Scholar
  12. Bennetzen, J.L. (1998) The structure and evolution of angiosperm nuclear genomes, Curr Opin Plant Biol 1, 103–108.PubMedGoogle Scholar
  13. Benyajati, C. and Worcel, A. (1976) Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster, Cell 9, 393–407.PubMedGoogle Scholar
  14. Bernardi, G. (1995) The human genome: organization and evolutionary history, Annu Rev Genet 29, 445–476.PubMedGoogle Scholar
  15. Bester, T.H. and Tycko, B. (1996) Creation of genomic methylation patterns, Nature Genet 12, 363–367.Google Scholar
  16. Bevan, M., Bennetzen, J.L., and Martienssen, R. (1998) Genome studies and molecular evolution commonalities, contrasts, continuity and change in plant genomes, Curr Opin Plant Biol 1, 101–102.PubMedGoogle Scholar
  17. Bilang, R. and Potrykus, I. (1998) Containing excitement over transplastomic plants, Nature Biotech 16, 333–334.Google Scholar
  18. Birch, R.G. (1997) Plant transformation: problems and strategies for practical application, Ann Rev Plant Physiol Plant Mol Biol 48, 297–326.Google Scholar
  19. Björklund, S., Almouzni, G., Davidson, I., Nightingale, K.P., and Weiss, K. (1999) Global transcription regulators of eukaryotes, Cell 96, 759–767.PubMedGoogle Scholar
  20. Boelens, W.C., Dargemont, C., and Mattaj, I.W. (1995) Export of mRNA through the nuclear pore complex, in A Lamond (ed.), Pre-mRNA Processing, R.G. Landes Publishers, Georgetown, TX, pp. 173–186.Google Scholar
  21. Borisjuk, N.V., Borisjuk, L.G., Logendra, S., Petersen, F., Gleba, Y., and Raskin, I. (1999) Production of recombinant proteins in plant root exudates, Nature Biotech 17, 466–469.Google Scholar
  22. Boston, R.S., Viitanen, P.V., and Vierling, E. (1996) Molecular chaperones and protein folding in plants, Plant Mol Bio132, 191–222.PubMedGoogle Scholar
  23. Bouchez, D. and Hofte, H. (1998) Functional genomics in plants, Plant Physiol 118, 725–732.PubMedGoogle Scholar
  24. Brendel, V., Carle-Urioste, J.C., and Walbot, V. (1998) Intron recognition in plants, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 20–28.Google Scholar
  25. Breyne, P., van Montagu, M., Depicker, N., Gheysen, G. (1992) Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco, Plant Cell 4, 463–471.PubMedGoogle Scholar
  26. Browning, K.S. (1996) The plant translational apparatus, Plant Mol Biol 32, 107–144.PubMedGoogle Scholar
  27. Browning, K.S., Goss, D.J., Roth, D.A., and Gallic, D.R. (1998) The translational machinery of plants, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 68–83.Google Scholar
  28. Bruick, R.K. and Mayfield, S.P. (1999) Light-activated translation of chloroplast mRNAs, TIPS 4, 190–195.Google Scholar
  29. Caddick, M.X., Greenland, A.J., Jepson, I., Krause, K.P., Qu, N., Riddell, K.V., Salter, M.G., Schuch, W., Sonnewald, U., and Tomsett, A.B. (1998) An ethanol inducible gene switch for plants used to manipulate carbon metabolism, Nature Biotech 16, 177–180.Google Scholar
  30. Cartes, N., Barakat, A., and Bernardi, G. (1995) The gene distribution of the maize genome, Proc Natl Acad Sci USA 92, 11057–11060.Google Scholar
  31. Cech, T.R. (1993) Ribozymes, in R.F. Gesteland and J.F. Atkin, (eds) The RNA World, Cold Spring Harbor Press, Cold Spring Harbor, NY, pp 239–269.Google Scholar
  32. Chinn, A.M., Payne, S.R., and Cornai, L. (1996) Variegation and silencing of the Heat Shock Cognate 80 gene are relieved by a bipartite downstream regulatory element, Plant J9, 325–339.PubMedGoogle Scholar
  33. Chiu, W., Niwa, Y., Zeng, W., Hirano, T., Kobayashi, H., and Sheen, J. (1996) Engineered GFP as a vital reporter in plants, Curr Biol 6, 325–330.PubMedGoogle Scholar
  34. Cogoni, C. and Macino, G. (1997) Conservation of transgene-induced post-transcriptional gene silencing in plants and fungi, TIPS 2, 438–443.Google Scholar
  35. Crameri, A., Whitehorn, E.A., Tate, E., and Stemmer, W.P.C. (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling, Nature Biotech 14, 315–319.Google Scholar
  36. Curtis, D., Lehmann, R., and Zamore, P.D. (1995) Translational regulation in development, Cell81, 171–178.PubMedGoogle Scholar
  37. Dale, P.J. and Irwin, J.A. (1995) The release of transgenic plants from containment, and the move towards their widespread use in agriculture, Euphytica 85, 425–43I.Google Scholar
  38. Daniell, H., Datta, R., Varma, S., Gray, S., and Lee, S.-B. (1998) Containment of herbicide resistance through genetic engineering of the chloroplast genome, Nature Biotech 16, 345–348.Google Scholar
  39. De Neve, M., De Buck, S., De Wilde, C., Van Houdt, H., Strobbe, I., Jacobs, A., Van Montagu, M., and Depicker, A. (1999) Gene silencing results in instability of antibody production in transgenic plants, Mol Gen Genet 260, 582–592.PubMedGoogle Scholar
  40. Depicker, A. and van Montagu, M. (1997) Post-transcriptional gene silencing in plants, Curr Opin Cell Biol 9, 373–382.PubMedGoogle Scholar
  41. DeScenzo, R.A. and Minocha, S.C. (1993) Modulation of cellular polyamines in tobacco by transfer and expression of mouse ornithine decarboxylase eDNA, Plant Mol Biol 22, 113–127.PubMedGoogle Scholar
  42. Doebley, J. and Lukens, L. (1998) Transcriptional regulators and the evolution of plant form, Plant Cell 10, 1075–1082.PubMedGoogle Scholar
  43. Düring, K., Porsch, P., Fladung, M., and Lörz, H. (1993) Transgenic potato plants resistant to the phytopathogenic bacterium Envinia carotovora, Plant J3, 587–598.Google Scholar
  44. Eckes, P., Schmitt, P., Daub, W., and Wengenmayer, F. (1989) Overexprcssion of alfalfa glutamine synthetase in transgenic tobacco plants, Mol Gen Genet 217, 263–268.PubMedGoogle Scholar
  45. Ellis, R.J., Dobson, C., and Hartl, U. (1998) Sequence does specify protein conformation, TIBS23, 468PubMedGoogle Scholar
  46. English, J.J., Davenport, G.F., Elmayan, T., Vaucheret, H., and Baulcombe, D.C. (1997) Requirement of sense transcription for homology-dependent virus resistance and trans-inactivation, Plant J 12, 597–604.Google Scholar
  47. Faiss, M., Zalubilova, J., Stmad, M., Schmülling, T. (1997) Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants, Plant J 12, 401–415.PubMedGoogle Scholar
  48. Filipowicz, W., Gniadkowski, M., Klahre, U., and Liu, H.-X. (1995) Pre-mRNA splicing in plants, in A. Lamond (ed.), Pre-mRNA Processing, R.G. Landes Publishers, Georgetown, TX, pp. 65–78.Google Scholar
  49. Fu, H., Kim, S.Y., and Park, W.D. (1995a) High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 5’ and 3’ flanking sequences and the leader intron, Plant Cell 7, 1387–1394.PubMedGoogle Scholar
  50. Fu, H., Kim, S.Y., and Park, W.D. (1995b) A potato Sus3 sucrose synthase gene contains a context-dependent 3’ element and a leader intron with both positive and negative tissue-specific effects, Plant Cell 7, 1395–1403.PubMedGoogle Scholar
  51. Fussenegger, M., Bailey, J.E., Hauser, H., and Mueller, P.P. (1999) Genetic optimization of recombinant glycoprotein production by mammalian cells, Trends Biotech 17, 35–42.Google Scholar
  52. Fütterer, J., Gordon, K., Bonneville, J.M., Sanfacon, H., Pisan, B., Penswick, J., and Hohn, T. (1988) The leading sequence ofcaulimovirus large RNA can be folded into a large stem-loop structure, Nucleic Acids Res 16, 8377–8390.PubMedGoogle Scholar
  53. Fütterer, J. and Hohn, T. (1996) Translation in plants — rules and exceptions, Plant Mol Biol 32, 159–189.PubMedGoogle Scholar
  54. Fütterer, J., Potrykus, 1., Valles-Brau, M.P., Dasgupta, I., Hull, R., and Hohn, T. (1994) Splicing in a plant pararetrovirus, Virology 198, 663–670.Google Scholar
  55. Gallie, D.R. (1996) Translational control of cellular and viral mRNAs, Plant Mol Biol 32, 145–158.PubMedGoogle Scholar
  56. Gallie, D.R. (1998a) Controlling gene expression in transgenics, Curr Opin Plant Biol 1, 166–172.PubMedGoogle Scholar
  57. Gallie, D.R. (1998b) A tale of two termini: a functional interaction between the termini of an mRNA is a prerequisite for efficient translation initiation, Gene 216, 1–11.PubMedGoogle Scholar
  58. Gatz, C. (1997) Chemical control of gene expression, Annu Rev Plant Physiol Plant Mol Biol 48, 89–108.PubMedGoogle Scholar
  59. Gatz, C., Frohberg, C., and Wendenburg, R. (1992) Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants, Plant J 2, 397–404.PubMedGoogle Scholar
  60. Gatz, C. and Lenk, I. (1998) Promoters that respond to chemical inducers, TIPS 3, 352–358.Google Scholar
  61. Ghoda, L., van Daalen, W.T., Macrae, M., Ascheruran, D., and Coffino, P. (1989) Prevention of rapid intracellular degradation of ODC by a carboxyl-terminal truncation, Science 243, 1493–1495.PubMedGoogle Scholar
  62. Goodall, G.J. and Filipowicz, W. (1991) Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants, EMBO J 10, 2635–2644.PubMedGoogle Scholar
  63. Grens, A. and Scheffler, I.E. (1990) The 5’- and 3’-untranslated regions of ornithine decarboxylase mRNA affect the translational efficiency, J Biol Chem 265, 11810–11816.PubMedGoogle Scholar
  64. Guilfoyle, T.J. (1997) The structure of plant gene promoters, Genet Eng 19, 15–47.Google Scholar
  65. Guo, Z. and Sherman, F. (1996) 3’-end-forming signals of yeast mRNA, TIBS 21, 477–481.Google Scholar
  66. Hamilton, C.M., Frary, A., Lewis, C., and Tanksley, S.D. (1996) Stable transfer of intact high molecular weight DNA into plant chromosomes, Proc Natl Acad Sci USA 93, 9975–9979.PubMedGoogle Scholar
  67. Haseloff, J., Siemering, K.R., Prasher, D.C., and Hodge, S. (1997) Removal of cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly, Proc Natl Acad Sci USA 94, 2122–2127.PubMedGoogle Scholar
  68. Heins, L., Collerison, I., and Soll, J. (1998) The protein translocation apparatus of chloroplast envelopes, TIPS 3, 5661.Google Scholar
  69. Hicks, G.R. and Raikhel, N.V. (1995) Protein import into the nucleus: an integrated view, Annu Rev Cell Dey Biol 11, 155–188.Google Scholar
  70. Hohn, T., Dominguez, D.I., Scharer-Hernandez, Pooggin, M.M., Schmidt-Puchta, W., Hemmings-Mieszczak, M., and Fütterer, J. (1998) Ribosome shunting in eukaryotes: what the viruses tell me, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 84–95.Google Scholar
  71. Holmes-Davis, R. and Cornai, L. (1998) Nuclear matrix attachment regions and plant gene expression, TIPS 3, 91–97.Google Scholar
  72. Holtorf, H., Schob, H., Kunz, C., Waldvogel, R., and Meins Jr., F. (1999) Stochastic and nonstochastic posttranscriptional silencing of chitinase and β-1,3-glucanase genes involves increased RNA turnover —possible role for ribosome-independent RNA degradation, Plant Cell 11, 471–483.PubMedGoogle Scholar
  73. Hunt, A.G., Chu, N.M., Odell, J.T., Nagy, F., and Chua, N. (1987) Plant cells do not properly recognize animal gene polyadenylation signals, Plant Mol Biol 8, 23–35.Google Scholar
  74. Hunt, A.G. and Messing, J. (1998) mRNA polyadenylation in plants, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 29–39.Google Scholar
  75. Iglesias, V.A., Moscone, E.A., Papp, I., Neuhuber, F., Michalowski, S., Phelan, T., Spiker, S., Matzke, M., and Matzke, A.J.M. (1997) Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco, Plant Cell 9, 1251–1264.PubMedGoogle Scholar
  76. Iida, S., Scheid, O.M., Saul, M.W., Seipel, K., Miyazaki, C., and Potrykus, I. (1992) Expression of a downstream gene from a bicistronic transcription unit in transgenic tobacco plants, Gene 119, 199–205.PubMedGoogle Scholar
  77. Johnson, M.A., Baker, E.J., Colbert, J.T., and Green, P.J. (1998) Determinants of mRNA stability in plants, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 40–53.Google Scholar
  78. Jorgensen, R.A., Que, Q., and Stam, M. (1999) Do unintended antisense transcripts contribute to sense cosuppression in plants? Trends Genet 15, 11–12.PubMedGoogle Scholar
  79. Joshi, C.P., Zhou, H., Huang, X., and Chiang, V.L. (1997) Context sequences of translation initiation codon in plants, Plant Mol Biol 35, 993–1001.PubMedGoogle Scholar
  80. Kahana, C. and Nathans, D. (1985) Nucleotide sequence of murine ornithine decarboxylase mRNA, Proc Natl Acad Sci USA 82, 1673–1677.PubMedGoogle Scholar
  81. Keller, W. (1995) 3’ end cleavage and polyadenylation of nuclear messenger RNA precursors, in A. Lamond (ed.), Pre-mRNA Processing, R.G. Landes Publishers, Georgetown, TX, pp. 113–128.Google Scholar
  82. Kempin, S.A. Liljegren, L.J., Block, L.M., Rounsly, S.D. and Yanofsky, M.F. (1997) Targeted disruption in Arabidopsis, Nature 389, 802–803.Google Scholar
  83. Klaff, P., Riesner, D., and Steger, G. (1996) RNA structure and the regulation of gene expression, Plant Mol Biol 32, 89–106.PubMedGoogle Scholar
  84. Klahre, UJ., Hemmings-Mieszczak, M., and Filipowicz, W. (1995) Extreme heterogeneity of polyadenylation sites in mRNAs encoding chloroplast RNA-binding proteins in Nicotiana plumbaginifolia, Plant Mol Biol 28, 569–574.PubMedGoogle Scholar
  85. Kozak, M. (1991) Structural features in eukaryotic mRNAs that modulate the initiation of translation, J Biol Chem 266, 19867–19870.PubMedGoogle Scholar
  86. Koziel, M.G., Carozzi, N.B., and Desai, N. (1996) Optimizing expression of transgenes with an emphasis on post-transcriptional events, Plant Mol Biol 32, 393–405.PubMedGoogle Scholar
  87. Kricker, M.C., Drake, J.W., and Radman, M. (1992) Duplication-targeted DNA methylation and mutagenesis in the evolution of eukaryotic chromosomes, Proc Natl Acad Sci USA, 89, 1075–1079.PubMedGoogle Scholar
  88. Ku, M.S.B., Agarie, S., Nomura, M., Fukayama, H., Tsuchida, 11., Ono, K., Hirose, S., Told, S., Miyao, M., and Matsuoka, M. (1999) High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants, Nature Biotech 17, 76–81.Google Scholar
  89. Kumpatla, S.P., Chandrasekharan, M.B., lyer, L.M., Li, G., and Hall, T.C. (1998) Genome intruder scanning and modulation systems and transgene silencing, TIPS 3, 97–104.Google Scholar
  90. Laroia, G., Cuesta, R., Brewer, G., and Schneider, R.J. (1999) Control of mRNA decay by heat shock-ubiquitinproteasome pathway, Science 284, 499–502.PubMedGoogle Scholar
  91. Leech, M.J., May, K., Hallard, D., Verpoorte, R., De Luca, V., and Christou, P. (1998) Expression of two consecutive genes of a secondary metabolic pathway in transgenic tobacco: molecular diversity influences levels of expression and product accumulation, Plant Mol Biol 38, 765–774.PubMedGoogle Scholar
  92. Lehmann, V. (1998) Patent on seed sterility threatens seed saving, Biotech Devel Monitor 35, 6–8.Google Scholar
  93. Lemmetyinen, J., Keinonen-Mettäla, Lännenpää, M., von Weissenberg, K., and Sopanen, T. (1998) Activity of the CaMV 355 promoter in various parts of transgenic early flowering birch clones, Plant Cell Rep 18, 243–248.Google Scholar
  94. Lewin, B. (1997) Genes VI, Oxford University Press, New York, NY, pp. 809–883.Google Scholar
  95. Lijegren, S.J. and Yanofsky, M.F. (1998)…Response: targeting Arabidopsis, TIPS 3, 79–80.Google Scholar
  96. Liu, B. and Alberts, B.M. (1995) Head-on collision between a DNA replication apparatus and RNA polymerase transcription complex, Science 267, 1131–1137.PubMedGoogle Scholar
  97. Lloyd, A.M., Schena, M., Walbot, V., and Davis, R.W. (1994) Epidermal cell fate determination in Arabidopsis: patterns defined by a steroid-inducible regulator, Science 266, 436–439.PubMedGoogle Scholar
  98. Lough, T., Tourneur, C., Masson, J., and Robaglia, C. (1997) Expression of genes in transgenic plants from bicistronic transcriptional units, Plant Sci 129, 91–99.Google Scholar
  99. Luehrsen, K.R., Taha, S., and Walbot, V. (1994) Nuclear pre-mRNA processing in higher plants, Prog Nucl Acid Res Mol Biol 47, 149–193.Google Scholar
  100. Maia, I.G., Séron, K., Haenni, A.-L., and Bernardi, F. (1996) Gene expression from viral RNA genomes, Plant Mol Biol 32, 367–391.PubMedGoogle Scholar
  101. Marcand, S., Gasser, S.M., and Gilson, E. (1996) Chromatin: a sticky silence, Curr Biol 6, 1222–1225.PubMedGoogle Scholar
  102. Marcotte Jr., W.R. (1998) Developmental regulation of translation and mRNA stability, in J. Bailey-Serres and D.R. Gal lie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 64–67.Google Scholar
  103. Martienssen, R.A. and Richards, E.J. (1995) DNA methylation in eukaryotes, Curr Opin Genet Dey 5, 234–242.Google Scholar
  104. Matsuoka, K. and Bednarek, S.Y. (1998) Protein transport within the plant cell endomembrane system: an update, Curr Opin Plant Biol 1, 463–469.PubMedGoogle Scholar
  105. Matzke, A.J.M. and Matzke, M.A. (1995) How and why do plants inactivate homologous (trans)genes?, Plant Physiol 107, 679–685.Google Scholar
  106. Matzke, A.J.M. and Matzke, M.A. (1998a) Position effects and epigenetic silencing of plant transgenes, Curr Opin Plant Biol 1, 142–148.PubMedGoogle Scholar
  107. Matzke, M.A. and Matzke, A.J.M. (1998b) Epigenetic silencing of plant transgenes as a consequence of diverse cellular defense responses, Cell Mol Life Sci 54, 94–103.PubMedGoogle Scholar
  108. Mayfield, S.P. and Cohen, A. (1998) Translational regulation in the chloroplast, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 174–179.Google Scholar
  109. McNellis, T.W., Mudgett, M.B., Li, K., Aoyama, T., Horvath, D., Chua, N.-H., and Staskawicz, B.J. (1998) Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death, Plant J 14, 247–257.PubMedGoogle Scholar
  110. Meins Jr., F. (1996) Epigenetic modifications and gene silencing in plants, in V.E.A. Russo, R.A. Martienssen, and A.D. Riggs (eds.), Epigenetic Mechanisms of Gene Regulation, Cold Spring Harbor Press, Cold Spring Harbor, NY, pp. 415–442.Google Scholar
  111. Meisel, L., and Lam, E. (1997) Switching on gene expression: analysis of the factors that spatially and temporally regulate plant gene expression, Genet Eng 19, 183–195.Google Scholar
  112. Mcshi, T. and lwabuchi, M. (1995) Plant transcription factors, Plant Cell Physiol 36, 1405–1420.Google Scholar
  113. Mett, V.L., Lochhead, L.B., and Reynolds, P.H.S. (1993) Copper controllable gene expression system for whole plants, Proc Nail Acad Sci USA 90, 4567–4571.Google Scholar
  114. Mett, V.L., Podivinsky, E., Tennant, A.M., Lochhead, L.P., Jones, W.T., and Reynolds, P.H. (1996) A system for tissue-specific copper-controllable gene expression in transgenic plants: nodule-specific antisensc expression of aspartate aminotransferase-P2, Transgen Res 5, 105–113.Google Scholar
  115. Mette, M.F., van der Winden, J., Matzke, M.A., and Matzke, A.J.M. (1999) Production of aberrant promoter transcripts contributes to methylation and silencing of unlinked homologous promoters in trans, EMBO J 18, 241–248.PubMedGoogle Scholar
  116. Meyer, P. (1995) Variation of transgene expression in plants, Euphytica 85, 359–366.Google Scholar
  117. Miao, Z.H. and Lam, E. (1995) Targeted disruption of the TGA3 locus in Arabidopsis thaliana, Plant J7, 359–365.PubMedGoogle Scholar
  118. Mlynárová, L., Loonen, A., Heldens, J. Jansen, R.C., Keizer, P., Stiekema, W.J., and Nap, J.-P. (1994) Reduced position effect in mature transgenic plants conferred by the chicken lysozyme matrix-associated region, Plant Cell 6, 417–426.PubMedGoogle Scholar
  119. Mlynârovâ, L., Jansen, R.E., Conner, A.J., Stiekema, W.J., and Nap, J.-P. (1995) The MAR-mediated reduction in position effect can be uncoupled from copy number-dependent expression in transgenic plants, Plant Cell 7, 599–609.PubMedGoogle Scholar
  120. Moffatt, A.S. (1998) Toting up the early harvest of transgenic plants, Science 282, 2176–2178.Google Scholar
  121. Moore, M.J., Query, C.C., and Sharp, P.A. (1993) Splicing of precursors to messenger RNAs by the spliceosomc, in R.F. Gestland and J. F. Atkins (eds.), The RNA World, Cold Spring Harbor Press, Cold Spring Harbor, NY, pp. 303–358.Google Scholar
  122. Motyka, V. Faiss, M., Strnad, M., Kaminek, M., and Schmülling (1996) Changes in cytokinin content and cytokinin oxidase activity in response to derepression of ipt gene transcription in transgenic tobacco calli and plants, Plant Physiol 112, 1035–1043.Google Scholar
  123. Muller, K.J., Romano, N., Gerstner, O., Garcia-Maroto, F., Pozzi, C., and Salamini, R.W. (1995) The barley Hooded mutation is caused by a duplication in a homebox gene intron, Nature 374, 727–730.PubMedGoogle Scholar
  124. Mulligan, R.M. and Maliga, P. (1998) RNA editing in mitochondria and plastids, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD pp. 153–161.Google Scholar
  125. Nagatani, A. (1998) Regulated nuclear targeting, Curr Opin Plant Biol 1, 470–474.PubMedGoogle Scholar
  126. Oakes, J.V., Shewmaker, C.K., and Stalker, D.M. (1991) Production of cyclodextrins, a novel carbohydrate, in the tubers of transgenic potato plants, Biotechnology 9, 982–986.PubMedGoogle Scholar
  127. Odell, J.T., Hoopes, J.L., and Vermerris, W. (1994) Seed-specific gene activation mediated by the cre/lox site-specific recombination system, Plant Physiol 106, 447–458.PubMedGoogle Scholar
  128. Okita, T. and Rogers, J. (1996) Compartmentation of proteins in the endomembrane system of plant cells, Annu Rev Plant Physiol Plant Mol Biol 47, 327–350.PubMedGoogle Scholar
  129. Oliver, M.J., Quisenberry, J.E., Trolinder, N.L.G., and Keim, D.L. (1998) Control of plant gene expression, Patent # 5,723,765, Delta and Pine Land Co (Scott, MS).Google Scholar
  130. Ow, D.W. (1996) Recombinase-directed chromosome engineering in plants, Curr Opin Bimechn 7, 181–186.Google Scholar
  131. Pabo, C.O. and Sauer, R.T. (1992) Transcription factors: structural families and principles ol DNA recognition, Annu Rev Biochem 61, 1053–1095.PubMedGoogle Scholar
  132. Pain, V.M. (1996) Initiation of protein synthesis in eukaryotic cells, Eur J Biochem 236, 747–771.PubMedGoogle Scholar
  133. Palauqui, J.-C. and Balzergue, S. (1999) Activation of systemic acquired silencing by localised introduction of DNA, Curr Biol 9, 59–66.PubMedGoogle Scholar
  134. Palauqui, J.-C., Elmayan, T., Pollien, J., and Vaucheret, H. (1997) Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions, EMBO J 16, 4738–4745.PubMedGoogle Scholar
  135. Palauqui, J.-C. and Vaucheret, H. (1998) Transgenes are dispensable for the RNA degradation step of cosuppression, Proc Nall Acad Sci USA 95, 9675–9680.Google Scholar
  136. Pegg, A.E. (1989) Characteristics of ornithine decarboxylase from various sources, in S.-I. Hayashi (ed.), Ornithine Decarboxylase: Biology, Enzymology, and Molecular Genetics, Pergamon Press, New York, pp. 21–34.Google Scholar
  137. Perlak, F.J., Fuchs, R.L., Dean, D.A., McPherson, S.L., and Fischhoff, D.A. (1991) Modification of the coding sequence enhances plant expression of insect control protein genes, Proc Nall Acad Sci USA 88, 3324–3328.Google Scholar
  138. Pesole, G., Liuni, S., Grillo, G., and Saccone, C. (1997) Structural and compositional features of untranslated regions of eukaryotic mRNAs, Gene 205, 95–102.PubMedGoogle Scholar
  139. Pestova, T.V. and Hellen, C.U.T. (1999) Ribosome recruitment and scanning: what’s new?, T/BS 24, 85–87.Google Scholar
  140. Proudfoot, N. (1989) How RNA polymerase II terminates transcription in higher eukaryotes, Trends Biochem Sci 14, 105–110.PubMedGoogle Scholar
  141. Puchta, H. (1998) Towards targeted transformation in plants, TIPS 3, 77–78.Google Scholar
  142. Puchta, H. and Hohn, B. (1996) From centiMorgans to basepairs: homologous recombination in plants, TIPS 1, 340348.Google Scholar
  143. Puchta, H., Swoboda, P. and Hohn, B. (1994) Homologous recombination in plants. Experientia, 50, 277–284.Google Scholar
  144. Radin, J.W. (1999) The technology protection system: revolutionary or evolutionary?, Biotech Devel Monitor 37, 24.Google Scholar
  145. Raikhel, N. (1992) Nuclear targeting in plants, Plant Physiol 100, 1627–1632.PubMedGoogle Scholar
  146. Ranish, J.A. and Hahn, S. (1996) Transcription: basal factors and activation, Curr Opin Genet Devel 6, 151–158.Google Scholar
  147. Ratcliff, F., Harrison, B. and Baulcombe, D. (1997) A similarity between viral defense and gene silencing in plants, Science 276, 1558–1560.PubMedGoogle Scholar
  148. Razin, A. (1998) CpG methylation, chromatin structure and gene silencing — a three-way connection, EMBO J 17, 4905–4908PubMedGoogle Scholar
  149. Richter, J.D. (1993) Translational control in development: a perspective, Devel Genet 14, 407–411.Google Scholar
  150. Rodriguez, C.M., Freire, M.A., Camilleri, C., and Robaglia, C. (1998) The Arabidopsis thaliana cDNAs coding for eIF4E and elF(iso)4E are not functionally equivalent for yeast complementation and are differentially expressed during plant development, Plant J 13, 465–473.PubMedGoogle Scholar
  151. Roesler, K.R., Shorrosh, B.S., and Ohlrogge, J.B. (1994) Structure and expression of an Arabidopsis acetyl-coenzyme A carboxylase gene, Plant Physiol 105, 611–617.PubMedGoogle Scholar
  152. Rossi, F.M.V. and Blau, H.M. (1998) Recent advances in inducible gene expression systems, Curr Opin Biotech 9, 451–456.PubMedGoogle Scholar
  153. Rothnie, H.M. (1996) Plant mRNA 3’-end formation, Plant Mol Biol 32, 43–61.PubMedGoogle Scholar
  154. Rouwendal, G.J.A., Mendes, O., Wolbert, E.J.H., and deBoer, A.D. (1997) Enhanced expression in tobacco of the gene encoding green fluorescent protein by modification of its codon usage, Plant Mol Biol 33, 989–999.PubMedGoogle Scholar
  155. Rugh, C.L., Senecoff, J.F., Meagher, R.B., and Merkle, S.A. (1998) Development of transgenic yellow poplar for mercury phytoremediation, Nature Biotech 16, 925–928.Google Scholar
  156. Rushton, P.J. and Somssich, I.E. (1998) Transcriptional control of plant genes responsive to pathogens, Curr Opin Plant Biol 1, 311–315.PubMedGoogle Scholar
  157. Schaefer, D.G. and Zryd, J.-P. (1997) Efficient gene targeting in the moss Physcomitrella patens, Plant J. 11, 11951206.Google Scholar
  158. Schuler, M.A. (1998) Plant pre-mRNA splicing, in J. Bailey-Serres and D.R. Gallie (eds.), A Look Beyond Transcription: Mechanisms Determining mRNA Stability and Translation in Plants, American Society of Plant Physiologists, Rockville, MD, pp. 1–19.Google Scholar
  159. Schwechheimer, C. and Bevan, M. (1998) The regulation of transcription factor activity in plants, TIPS 3, 378–383.Google Scholar
  160. Schwechheimer, C., Smith, C., and Bevan, M.W. (1998) The activities of acidic and glutamine-rich transcriptional activation domains in plant cells: design of modular transcription factors for high-level expression, Plant Mol Biol36, 195–204.PubMedGoogle Scholar
  161. Seraphin, B. (1995) Sm and Sm-like proteins belong to a large family: identification of proteins of U6 as well as the U1, U2 U4 and U5snRNPs, EMBO J14, 2089–2098.Google Scholar
  162. Shantz, L.M., Viswanath, R., and Pegg, A.E. (1994) Role of the 5’-untranslated region of mRNA in the sysnthesis of S-adenosylmethionine decarboxylase and its regulation by spermine, Biochem J 302. 765–772.PubMedGoogle Scholar
  163. Simpson, G.G. and Filipowicz, W. (1996) Splicing of precursors to messenger RNA in higher plants: mechanism, regulation and sub-nuclear organization of the spliceosomal machinery, Plant Mol Biol 32, 1–41.PubMedGoogle Scholar
  164. Sinibaldi, R.M. and Mettler, I.J. (1992) Intron splicing and intron-mediated enhanced gene expression in monocots, Progr Nucl Acid Res Mol Biol 42, 229–257.Google Scholar
  165. Smith, H.M.S. and Raikhel, N.V. (1999) Protein targeting to the nuclear pore. What can we learn from plants?, Plant Physiol 119, 1157–1163.PubMedGoogle Scholar
  166. Spiker, S. and Thompson, W.F. (1996) Nuclear matrix attachment regions and transgene expression in plants. Plant Physiol 110, 15–21.PubMedGoogle Scholar
  167. Suzuki, T., Yan, Q., and Lennarz, W.J. (1998) Complex, two-way traffic of molecules across the membrane of the endoplasmic reticulum, J Biol Chem 273, 10083–10086.PubMedGoogle Scholar
  168. Thieffry, D. and Sarkar, S. (1998) Forty years under the central dogma, TIBS 23, 312–316.PubMedGoogle Scholar
  169. Thomas, K.R., Deng, C., and Capecchi, M.R. (1992) High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors, Mol Cell Biol 12, 2919–2923.PubMedGoogle Scholar
  170. Thykjaer, T., Finnemann, J., Schauser, L., Christensen, L., Poulsen, C., and Stougaard, J. (1997) Gene targeting approaches using positive-negative selection and large flanking regions, Plant Mol Biol 35, 523–530.PubMedGoogle Scholar
  171. Tinland, B. (1996) The integration of T-DNA in plant genomes, TIPS 1, 178–184.Google Scholar
  172. Udvardy, A. (1999) Dividing the empire: boundary chromatin elements delimit the territory of enhancers, EMBO J, 18, 1–8.PubMedGoogle Scholar
  173. Valcarel, J., Singh, R., and Green, M.R. (1995) Mechanisms of regulated pre-mRNA splicing, in A. Lamond (ed.), Pre-mRNA Processing, R.G. Landes Publishers, Georgetown, TX, pp. 97–112.Google Scholar
  174. van Aarssen, R., Soetaert, P., Stain, M., Dockx, J., Gosselé, V., Seurinck, J., Reynaerts, A., and Cornelissen, M. (1995) crylA(b) transcript formation in tobacco is inefficient, Plant Mol Biol 28, 513–524.Google Scholar
  175. van der Geest, A.H.M. and Hall, T.C. (1997) The ß-phaseolin 5’ matrix attachment region acts as an enhancer facilitator, Plant Mol Biol 33, 553–557.PubMedGoogle Scholar
  176. van Engelen, F.A., Schouten, A., Molthoff, J.W., Roosien, J., Salinas, J., Dirkse, W.G., Schots, A., Bakker, J., Gommers, F.J., Jongsma, M.A., Bosch, D., and Stiekema, W.J. (1994) Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco, Plant Mol Biol 26, 1701–1710.PubMedGoogle Scholar
  177. van Steeg, H., Van Oostrom, C.T., Hodemaekers, H.M., Peters, L., and Thomas, A.A. (1991) The translation in vitro of rat ornithine decarboxylase mRNA is blocked by its 5’ untranslated region in a polyamine-independent way, Biochem J274, 521–526.PubMedGoogle Scholar
  178. Vaucheret, H., Nussaume, L., Palauqui, J.-C., Quilleré, 1., and Elmayan, T. (1997) A transcriptionally active state is required for post-transcriptional silencing (co-suppression) of nitrate reductase host genes and transgenes, Plant Cell 9, 1495–1504.Google Scholar
  179. Verwoerd, T.C., van Paridon, P.A., van Ooyen, A.J.J., van Lent, J.W.M., Hoekema, A., and Pen, J. (1995) Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves, Plant Physiol 109, 1199–1205.PubMedGoogle Scholar
  180. Vitale, A. and Raikhel, N.V. (1999) What do proteins need to reach different vacuoles?, TIPS 4, 149–155.Google Scholar
  181. Voinnet, O., Vain, P., Angell, S., and Baulcombe, D.C. (1998) Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoter-less DNA, Cell 177, 187.Google Scholar
  182. von Hippel, P.H. (1998) An integrated model of the transcription complex in elongation, termination, and editing, Science 281, 660–665.Google Scholar
  183. Walbot, V. (1999) Genes, genomes, genomics. What can plant biologists expect from the 1998 National Science Foundation plant genome program? Plant Physiol 119, 1151–1155.PubMedGoogle Scholar
  184. Wang, S., Browning, K.S., and Miller, W.A. (1997) A viral sequence in the 3’-untranslated region mimics a 5’ cap in facilitating translation of uncapped mRNA, EMBO J 13, 4107–4116.Google Scholar
  185. Wassenegger, M. and Pélissier, T. (1998) A model for RNA-mediated gene silencing in higher plants, Plant Mol Biol 37, 349–362.PubMedGoogle Scholar
  186. Wassenegger, M. and Pélissier, T. (1999) Signalling in gene silencing, TIPS 4, 207–209.Google Scholar
  187. Weihe, A. and Börner (1999) Transcription and the architecture of promoters in chloroplasts, TIPS 4, 169–170.Google Scholar
  188. Weinmann, P., Gossen, M., Bitten, W., Bujard, H. and Gatz, C. (1994) A chimeric transactivator allows tetracycline-response gene expression in whole plants, Plant J 5, 559–569.PubMedGoogle Scholar
  189. Williamson, J.D., Hirsch-Wyncott, M.E., Larkins, B.A., and Gelvin, S.B. (1989) Differential accumulation of a transcript driven by the CaMV 35S promoter in transgenic tobacco, Plant Physiol 90, 1570–1576.PubMedGoogle Scholar
  190. Wolffe, A.P. (1994) Nucleosome positioning and modification: chromatin structures that potentiate transcription, TIBS 19, 240–244.PubMedGoogle Scholar
  191. Yoder, J.A., Walsh, C.P., and Bestor, T.H. (1997) Cytosine methylation and the ecology of intragenomic parasites, Trends Genet 13, 335–340.PubMedGoogle Scholar
  192. Zhou, D.-X. (1999) Regulatory mechanism of plant gene transcription by GT-elements and GT-factors, TIPS 4, 210–214.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2000

Authors and Affiliations

  • Subhash C. Minocha
    • 1
  1. 1.Department of Plant BiologyUniversity of New HampshireDurhamUSA

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