Abstract
A PCR DNA diagnostics system based on a hybrid microfluidic platform is described which offers the advantage of disposability for high DNA concentration PCR. PCR results for a 100 μ1 solution of M13 Bacteriophage DNA as well as a β-Actin human DNA TaqMan® assay show positive amplification after automated sample preparation in the block for both cases. Results have also shown successful follow-up decontamination of the reusable platform after each sample preparation and amplification run. The design and processes utilized in this hybrid system has been leveraged to design and develop a fully disposable cartridge platform for detection of Chlamydia and Gonorrhea from urine samples.
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© 2000 Springer Science+Business Media Dordrecht
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Pourahmadi, F. et al. (2000). Versatile, Adaptable and Programmable Microfluidic Platforms for DNA Diagnostics and Drug Discovery Assays. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_56
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DOI: https://doi.org/10.1007/978-94-017-2264-3_56
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5496-8
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