Abstract
Five streptococcal integration vectors containing different antibiotic resistance-encoding genes capable of expressing in both Streptococcus sp. and Escherichia coli are introduced. These plasmids can replicate in E. coli, but not in streptococci, because the plasmids lack a streptococcal origin of replication. If these plasmids carry a fragment of streptococcal DNA they can specifically integrate into the streptococcal chromosome via Campbell-like, single crossover homologous recombination. Methods are described to use these vectors for gene inactivation and cloning in streptococci.
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Abbreviations
- Ap:
-
ampicillin
- bp:
-
base pair(s)
- Cm:
-
chloramphenicol
- Em:
-
erythromycin
- kb:
-
kilobase(s) or 1000 bp
- Km:
-
kanamycin
- ori :
-
origin of DNA replication
- R :
-
resistant/resistance
- Sp:
-
spectinomycin
- Tc:
-
tetracycline
- TSS:
-
transformation and storage solution
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© 1998 Springer Science+Business Media Dordrecht
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Tao, L. (1998). Streptococcal integration vectors for gene inactivation and cloning. In: Fives-Taylor, P.M., LeBlanc, D.J. (eds) Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2258-2_6
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DOI: https://doi.org/10.1007/978-94-017-2258-2_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5262-9
Online ISBN: 978-94-017-2258-2
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