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Lactic Acid Bacteria: Genetics, Metabolism and Applications pp 123–132Cite as

Genome plasticity in Lactococcus lactis

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Abstract

Comparative genome analyses contribute significantly to our understanding of bacterial evolution and indicate that bacterial genomes are constantly evolving structures. The gene content and organisation of chromosomes of lactic acid bacteria probably result from a strong evolutionary pressure toward optimal growth of these microoranisms in milk. The genome plasticity of Lactococcus lactis was evaluated at inter- and intrasubspecies levels by different experimental approaches. Comparative genomics showed that the lactococcal genomes are not highly plastic although large rearrangements (a.o. deletions, inversions) can occur. Experimental genome shuffling using a new genetic strategy based on the Cre-loxP recombination system revealed that two domains are under strong constraints acting to maintain the original chromosome organisation: a large region around the replication origin, and a smaller one around the putative terminus of replication. Future knowledge of the rules leading to an optimal genome organisation could facilitate the definition of new strategies for industrial strain improvement.

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Authors and Affiliations

  1. Laboratoire de Microbiologie et Génétique Moléculaire du CNRS (UMR5100), Université Paul Sabatier, 31062, Toulouse, France

    Nathalie Campo, Miguel J. Dias, Marie-Line Daveran-Mingot, Paul Ritzenthaler & Pascal Le Bourgeois

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  1. Nathalie Campo
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  2. Miguel J. Dias
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  3. Marie-Line Daveran-Mingot
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  4. Paul Ritzenthaler
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  5. Pascal Le Bourgeois
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Editor information

Editors and Affiliations

  1. Wageningen Center for Food Sciences, and NIZO food research, the Netherlands

    R. J. Siezen

  2. University of Groningen, the Netherlands

    J. Kok

  3. Wageningen Center for Food Sciences, and Wageningen University, the Netherlands

    T. Abee

  4. TNO Nutrition and Food Research, Zeist, the Netherlands

    G. Schasfsma

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© 2002 Springer Science+Business Media Dordrecht

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Campo, N., Dias, M.J., Daveran-Mingot, ML., Ritzenthaler, P., Le Bourgeois, P. (2002). Genome plasticity in Lactococcus lactis . In: Siezen, R.J., Kok, J., Abee, T., Schasfsma, G. (eds) Lactic Acid Bacteria: Genetics, Metabolism and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2029-8_8

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  • DOI: https://doi.org/10.1007/978-94-017-2029-8_8

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6141-6

  • Online ISBN: 978-94-017-2029-8

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