Application and Evaluation of Entomopathogens in Crucifers and Cucurbits

  • John D. Vandenberg
  • Anthony M. Shelton
  • Stephen P. Wraight

Abstract

Cruciferous and cucurbitaceous vegetable row crops are comprised of many species and varieties and are cultivated worldwide in many climates. In this chapter, we present a brief overview of selected pests and microbial control agents that are effective against some of them. We confine our remarks to crops grown in open field settings. Management of pests of crops grown in protected environments (e.g., under glass) is covered in Chapter VII-8. Using lepidopterous pests of crucifers and whitefly pests of cucurbits as examples, we will present protocols for the application and evaluation of microbial control agents, with special emphasis on the fungus Beauveria bassiana.

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References

  1. Anonymous 1999. “Crop Protection Reference.” 15th ed. C & P Press, New York.Google Scholar
  2. Baur, M. E., Kaya, H. K., Gaugler, R., and Tabashnik, B. 1997. Effects of adjuvants on entomopathogenic nematode persistence and efficacy against Plutella xylostella. Biocontr. Sci. Technol. 7, 513–525.CrossRefGoogle Scholar
  3. Baur, M. E., Kaya, H. K., Tabashnik, B. E., and Chilcutt, C. F. 1998. Suppression of diamondback moth (Lepidoptera: Plutellidae) with an entomopathogenic nematode (Rhabditida: Steinernematidae) and Bacillus thuringiensis Berliner. J. Econ. Entomol. 91, 1089–1095.PubMedGoogle Scholar
  4. Brewer, M. J. and Story, R. N. 1987. Larval spatial patterns and sequential sampling plan for pickleworm, Diaphania nitidalis (Stoll) (Lepidoptera: Pyralidae), on summer squash. Environ. Entomol. 16, 539–544.Google Scholar
  5. Cao, J., Tang, J. D., Strizhov, N., Shelton, A. M., and Earle, E. D. 1999. Transgenic broccoli with high levels of Bacillus thuringiensis Cry1C protein control diamondback moth larvae resistant to CrylA or Cry1C. Mol. Breeding 5, 131–141.CrossRefGoogle Scholar
  6. Choo, H. Y., Koppenhöfer, A. M., and Kaya, H. K. 1996. Combination of two entomopathogenic nematode species for suppression of an insect pest. J. Econ. Entomol. 89, 97–103.Google Scholar
  7. Clark, R. A., Casagrande, R. A., and Wallace, D. B. 1982. Influence of pesticides on Beauveria bassiana, a pathogen of the Colorado potato beetle. Environ. Entomol. 11, 67–70.Google Scholar
  8. Cunningham, J. T. 1998. North America. In “Insect Viruses and Pest Management” ( Hunter-Fujita, F. R., Entwistle, P. F., Evans, H. F. and Crook, N. E., Eds.), pp. 313–331. Wiley, New York.Google Scholar
  9. Edelson, J. V. 1986. Comparison of sampling methods for insect pests of canteloupe. J. Econ. Entomol. 79, 266–270.Google Scholar
  10. Federici, B. A. 1999. Naturally occurring baculoviruses for insect pest control. In “Methods in Biotechnology, vol. 5: Biopesticides: Use and Delivery” ( Hall, F. R. and Menn, J. J., Eds.), pp. 301–320. Humana Press, Totawa, NJ.Google Scholar
  11. Feng, M. G., Poprawski, T. J., and Khachatourians, G. G. 1994. Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: Current status. Biocontrol Sci. and Technol. 4, 3–34.CrossRefGoogle Scholar
  12. Finch, S. and Thompson, A. R. 1992. Pests of cruciferous crops. In “Vegetable Crop Pests” ( McKinlay, R. G., Ed.) pp. 87–138. CRC Press, Boca Raton, FL.Google Scholar
  13. Furlong, M. J., Pell, J. K., Choo, O. P., and Rahman, S. A. 1995. Field and laboratory evaluation of a sex pheromone trap for the autodissemination of the fungal entomopathogen Zoophthora radicans by the diamondback moth, Plutella xylostella. Bull. Entomol. Res. 85, 331–337.CrossRefGoogle Scholar
  14. Goettel, M. S. and Inglis, G. D. 1997. Fungi: Hyphomycetes. In “Manual of Techniques in Insect Pathology” ( Lacey, L. A., Ed.) pp. 213–249. Academic Press, London.CrossRefGoogle Scholar
  15. Greene, G. L., Genung, W. C., Workman, R. B., and Kelsheimer, E. G. 1969. Cabbage looper control in Florida–a cooperative program. J. Econ. Entomol. 62, 798–800.Google Scholar
  16. Henderson, C. F. and Tilton, E. 1955. Test with acaricides against the brown wheat mite. J. Econ. Entomol. 18, 265–267.Google Scholar
  17. Huber, J. 1998. Western Europe. In “Insect Viruses and Pest Management” ( Hunter-Fujita, F. R., Entwistle, P. F., Evans, H. F. and Crook, N. E., Eds.), pp. 201–215. Wiley, New York.Google Scholar
  18. Humber, R. A. 1997. Fungi: Identification. In “Manual of Techniques in Insect Pathology” ( Lacey, L. A., Ed.) pp. 153–185. Academic Press, London.CrossRefGoogle Scholar
  19. Ibrahim, Y. B. and Low, W. 1993. Potential of mass-production and field efficacy of isolates of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus against Plutella xylostella. Int. J. Pest Manag. 39, 288–292.CrossRefGoogle Scholar
  20. Janos-Su, J., Groden, E., and Zhang, J. 1999. Effects of selected fungicides and the timing of fungicide application in Beauveria bassiana-induced mortality of the Colorado potato beetle (Coleoptera: Chrysomelidae). Biol. Contr. 15, 259–269.CrossRefGoogle Scholar
  21. Jaronski, S. T. and Lord, J. C. 1996. Evaluation of Beauveria bassiana (Mycotrol® WP) for control of whitefly in spring cantaloupes. Arthropod Manag. Tests 21, 103.Google Scholar
  22. Kadir, H. A. 1992. Potential of several baculoviruses for the control of diamondback moth and Crocidolomia binotalis on cabbages. In “Diamondback moth and other crucifer pests: Second international workshop, 1990” ( Talekar, N. S., Ed.) pp. 185–192. Asian Vegetable Research and Development Center, Tainan, Taiwan.Google Scholar
  23. Kaya, H. K., Burlando, T. M., Choo, H. Y., and Thurston, G. S. 1995. Integration of entomopathogenic nematodes with Bacillus thuringiensis or pesticidal soap for control of insect pests. Biol. Contr. 5, 432–441.CrossRefGoogle Scholar
  24. Lacey, L. A., Fransen, J. J., and Carruthers, R. 1996. Global distribution of naturally occurring fungi of Bemisia, their biologies and use as biological control agents. In “Bemisia 1995: Taxonomy, Biology, Damage, and Management” (Gerling, D. and Mayer, R., Eds.) pp. 401–433. Intercept, Andover, UK.Google Scholar
  25. Little, T. M. and Hills, F. J. 1978. “Agricultural Experimentation: Design and Analysis.” Wiley, NY.Google Scholar
  26. Lorin, R., Galaini, S., and Roberts, D. W. 1983. Survival of inoculum of the entomopathogenic fungus Beauveria bassiana as influenced by fungicides. Environ. Entamai. 12, 1724–1726.Google Scholar
  27. Lynch, R. E. and Simmons, A. M. 1993. Distribution of immatures and monitoring of adult sweetpotato whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) in peanut, Arachis hypogaea. Environ. Entomol. 22, 375–380.Google Scholar
  28. Metz, R. D., Roush, R. T., Tang, J. D., Shelton, A. M., and Earle, E. D. 1995. Transgenic broccoli expressing a Bacillus thuringiensis insecticidal crystal protein: implications for pest resistance management strategies. Mol. Breeding 1, 309–317.CrossRefGoogle Scholar
  29. Moscardi, F. 1999. Assessment of the application of baculoviruses for control of Lepidoptera. Annu. Rev. Entomol. 44, 257–289.PubMedCrossRefGoogle Scholar
  30. Naranjo, S. E. and Flint, H. M. 1994. Spatial distribution of preimaginal Bemisia tabaci (Homoptera:Aleyrodidae) in cotton and development of fixed-precision sequential sampling plans. Environ. Entomol 23, 254–266.Google Scholar
  31. Pell, J. K., Macaulay, E. D. M., and Wilding, N. 1993. A pheromone trap for dispersal of the pathogen zoophthora radicans Brefeld. (Zygomycetes: Entomophthorales) amongst populations of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae). Biocontr. Sci. Technol. 3 315–320.Google Scholar
  32. Perez, C. J., Shelton, A. M., and Derksen, R. C. 1995. Effect of application technology and Bacillus thuringiensis Subspecies on management of B. thuringiensis subsp. kurstaki-resistant diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol. 88, 1113–1119.Google Scholar
  33. Perez, C. J., Shelton, A. M., and Erickson, N. 1996. Testing for water pH effect on Bacillus thuringiensis toxicity to diamondback moth. Arthropod Manag. Tests 21, 399–400.Google Scholar
  34. Reiners, S., Petzoldt, C. H., Hoffmann, M. P., and Schoenfeld, C. C., Eds. 1999. “Integrated crop and pest management recommendations for commercial vegetable production.” Cornell University Cooperative Extension, Ithaca, New York. 305 pp.Google Scholar
  35. Schroeder, P. C., Ferguson, C. S., Shelton, A. M., Wilsey, W. T., Hoffman, M. P., and Petzoldt, C. 1996. Greenhouse and field evaluations of entomopathogenic nematodes (Nematoda: Hetrorhabditidae and Steinemematidae) for Control of cabbage maggot (Diptera: Anthomyiidae) on cabbage. J. Econ. Entomol. 89, 1109–1115.PubMedGoogle Scholar
  36. Sears, M. K., Shelton, A. M., Quick, T. C., Wyman, J. A., and Webb, S. E. 1985. Evaluation of partial plant sampling procedures and corresponding action thresholds for management of Lepidoptera on cabbage. J. Econ. EntomoL 78, 913–916.Google Scholar
  37. Shah, P. A. and Goettel, M. S., Eds. 1998. “Directory of microbial control products and services.” Society for Invertebrate Pathology, Division of Microbial Control, Gainesville, Florida. 26 pp.Google Scholar
  38. Shelton, A. M., Andaloro, J. T., and Barnard, J. 1982. Effects of cabbage looper, imported cabbageworm, and diamondback moth on fresh market and processing cabbage. J. Econ. Entomol 75, 742–745.Google Scholar
  39. Shelton, A. M., Kroening, M. K., Eigenbrode, S. D., Petzold, C., Hoffmann, M. P., Wyman, J. A., Wilsey, W. T., Cooley, R. J., and Pedersen, L. H. 1996. Diamondback moth (Lepidoptera: Plutellidae) contamination of cabbage transplants and the potential for insecticide resistance problems. J. Entomol. Sci. 31, 347–354.Google Scholar
  40. Shelton, A. M., Robertson, J. L., Tank, J. D., Perez, C., Eigenbrode, S. D., Preisler, H. K., Wilsey, W. T., and Cooley, R. J. 1993. Resistance of diamonback moth (Lepidoptera: Plutellidae) to Bacillus thuringiensis subspecies in the field. J. Econ. Entomol. 86, 697–705.Google Scholar
  41. Shelton, A. M., Sears, M. K., Wyman, J. A., and Quick, T. C. 1983. Comparison of action thresholds for lepidopterous larvae on fresh market cabbage. J. Econ. Entomol 76, 196–199.Google Scholar
  42. Shelton, A. M., Vandenberg, J. D., Ramos, M., and Wilsey, W. T. 1998. Efficacy and persistence of Beauveria bassiana and other fungi for control of diamondback moth (Lepidoptera: Plutellidae) on cabbage seedlings. J. Entomol. Sci. 33, 142–151.Google Scholar
  43. Shelton, A. M., Wilsey, W. T., and Schmaedick, M. A. 1998. Management of onion thrips on cabbage using plant resistance and insecticides. J. Econ. EntomoL 91, 329–333.Google Scholar
  44. Sokal, R. R. and Rohlf, F. J. 1995. “Biometry.” 3rd ed. Freeman, New York. 887 pp.Google Scholar
  45. Tabashnik, B. E., Cushing, N. L., Finson, N., and Johnson, M. W. 1990. Field development of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol 83, 1671–1676.Google Scholar
  46. Talekar, N. S. and Shelton, A. M. 1993. Biology, ecology and management of the diamondback moth. Ann. Rev. Entomol 38, 275–302.CrossRefGoogle Scholar
  47. Todorova, S. I., Coderre, D., Duchesne, R. M., and Cote, J. C. 1998. Compatibility of Beauveria bassiana with selected fungicides and herbicides. Environ. Entomol. 27, 427–433.Google Scholar
  48. Tonhasca, A., Jr., Palumbo, J. C., and Byrne, D. N. 1994. Distribution patterns of Bemisia tabaci (Homoptera: Aleyrodidae) in cantaloupe fields in Arizona. Environ. Entomol. 23, 949–954.Google Scholar
  49. Treacy, M. F. 1999. Recombinant baculoviruses. In “Methods in Biotechnology, vol. 5: Biopesticides: Use and Delivery” ( Hall, F. R. and Menn, J. J., Eds.), pp. 321–340. Humana Press, Totawa, NJ.Google Scholar
  50. Treacy, M. F., All, J. N., and Ghidiu, G. M. 1997. Effect of ecdysteroid UDP-glucosyltransferase gene deletion on efficacy of a baculovirus against Heliothis virescens and Trichoplusia ni (Lepidoptera: Noctuidae). J. Econ. Entomol. 90, 1207–1214.PubMedGoogle Scholar
  51. Vandenberg, J. D., Shelton, A. M., Wilsey, W. T., and Ramos, M. 1998. Assessment of Beauveria bassiana sprays for control of diamondback moth (Lepidoptera: Plutellidae) on crucifers. J. Econ. Entomol. 91, 624–630.Google Scholar
  52. Webb, S. E. and Shelton, A. M. 1990. Effect of age structure on the outcome of viral epizootics in field populations of imported cabbageworm. Environ. Entomol. 19, 111–116.Google Scholar
  53. Webb, S. E. and Shelton, A. M. 1991. A simple action threshold for timing applications of a granulosis virus to control Pieris rapae. Entomophaga 36, 379–389.CrossRefGoogle Scholar
  54. Wood, H. A., Hughes, P. R., and Shelton, A. 1994. Field studies of the co-occlusion strategy with a genetically altered isolate of the Autographa californica nuclear polyhedrosis virus. Environ. Entomol. 23, 211–219.Google Scholar
  55. Wraight, S. P. and Bradley, C. A. 1996. Use of Mycotrol (Beauveria bassiana) for biological control of Bemesia argentifolii (Homoptera: Aleyrodidae) in field crops. In “Technology transfer in biological control: from research to practice” (Silvy, C., Ed.) pp. 151. IOBC WPRS Bulletin 19 (8), Montpellier, France.Google Scholar
  56. Wraight, S. P. and Carruthers, R. I. 1999. Production, delivery and use of mycoinsecticides for control of insect pests of field crops. In “Biopesticides: Use and Delivery” ( Hall, F. and Menn, J., Eds.), pp. 233–269. Humana Press, Totowa, NJ.Google Scholar
  57. Wraight, S. P., Carruthers, R. I., and Bradley, C. A. 1996. Development of entomopathogenic fungi for microbial control of whiteflies of the Bemisia tabaci complex. In “Simposio de Controle Biologico, Avais: Conferëncias e Palestras.” pp. 28–34. V SICONBIOL, Foz do Iguaçu, Brazil.Google Scholar
  58. Wraight, S. P., Carruthers, R. I., Jaronski, S. T., Bradley, C. A., Garza, C. J., and Galaini-Wraight, S. 2000. Evaluation of the entomopathogenic fungi, Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly, Bemesia argentifolii. Biol. Contr. 17 (in press).Google Scholar
  59. York, A. 1992. Pests of cucurbit crops: marrow, pumpkin, squash, melon and cucumber. In “Vegetable Crop Pests” ( McKinlay, R. G., Ed.) pp. 139–161. CRC Press, Boca Raton, FL.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2000

Authors and Affiliations

  • John D. Vandenberg
    • 1
  • Anthony M. Shelton
    • 2
  • Stephen P. Wraight
    • 1
  1. 1.USDA ARS, U. S. Plant, Soil & Nutrition LaboratoryPlant Protection Research UnitIthacaUSA
  2. 2.Department of EntomologyCornell UniversityGenevaUSA

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