Abstract
The effect of column verticality on liquid dispersion and separation efficiency in expanded bed adsorption columns was investigated using 1 and 5 cm diameter columns. Column misalignment of only 0.15° resulted in the reduction of the Bodenstein number from 140 to 50 for the 1 cm dia. column and from 75 to 45 for the 5 cm dia. column. This degree of misalignment was not detectable by visual assessment of adsorbent particle movement within the column. Depending on the relative importance of transport limitations, kinetic limitations and dispersion to any specific separation, this increase in dispersion with column alignment can significantly affect separation efficiency. Pure protein breakthrough profiles resulting from the application of bovine serum albumin onto STREAMLINE Q XL demonstrated that, at 10% breakthrough, 7.8% more protein could be applied to a vertical 1 cm dia. column compared to the same column misaligned by 0.15°. When an unclarified yeast homogenate was applied to a 1 cm dia. vertical column packed with STREAMLINE DEAE, 10% breakthrough of glucose-6-phosphate dehydrogenase (G6PDH) corresponded to a load 55% greater compared to the same column aligned 0.185° off-vertical. The G6PDH breakthrough curves for vertical and 0.15° off-vertical runs performed using a 5 cm column were essentially indistinguishable.
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References
Bruce LJ and Chase HA (1998) Monitoring of glucose-6-phosphate dehydrogenase breakthrough curves within an expanded bed adsorption column. Bioseparation, this volume.
Hudson C, Briens CL and Prakash A (1996) Effect of inclination on liquid-solid fluidized beds. Powder Tech. 89: 101 - 113.
Johansson U (1994) Hydrodynamics of a liquid-solid fluidized bed in the low Reynolds number region. Licentiate thesis. Royal Institute of Technology, Stockholm, Sweden.
Karau A, Benken C, Thommes J and Kula M-R (1997) The influence of particle size distribution and operating conditions on the adsorption performance in fluidized bed. Biotech. Bioeng. 55: 54-64.
Levenspiel 0 (1972) Chemical Reaction Engineering. Wiley, New York.
McCreath GE, Chase HA and Lowe CR (1994) Novel affinity separations based on perfluorocarbon emulsions. J. Chrom. A. 659: 275-287.
Van der Meer AP, Blanchard CMRJP and Wesselingh JA (1984) Mixing of particles in liquid fluidised beds. Chem. Eng. Des. Res. 64: 214-222.
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© 1999 Springer Science+Business Media Dordrecht
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Bruce, L.J., Ghose, S., Chase, H.A. (1999). The effect of column verticality on separation efficiency in expanded bed adsorption. In: Mattiasson, B. (eds) Expanded Bed Chromatography. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1519-5_6
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DOI: https://doi.org/10.1007/978-94-017-1519-5_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5380-0
Online ISBN: 978-94-017-1519-5
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