Abstract
The technique of expanded bed adsorption is originally designed for a direct recovery of proteins from fermentor feedstocks. In this article we describe the use of expanded bed adsorption for the recovery of a-lactalbumins from defatted milk using the hydrophobic Streamline Phenyl gel. a-Lactalbumins are Ca2+- binding proteins. Upon Ca2+ removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique property we develop a protocol for fast and efficient purification of α-lactalbumin from milk. The use of this technique results in a reduction of the number of chromatographic purification steps.
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Abbreviations
- BLA:
-
bovine α-lactalbumin
- CIP:
-
cleaning in place
- EBA:
-
expanded bed adsorption
- GLA:
-
goat a-lactalbumin
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© 1999 Springer Science+Business Media Dordrecht
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Noppe, W., Haezebrouck, P., Hanssens, I., De Cuyper, M. (1999). A simplified purification procedure of α-lactalbumin from milk using Ca2+-dependent adsorption in hydrophobic expanded bed chromatography. In: Mattiasson, B. (eds) Expanded Bed Chromatography. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1519-5_17
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DOI: https://doi.org/10.1007/978-94-017-1519-5_17
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5380-0
Online ISBN: 978-94-017-1519-5
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