Abstract
Efficient design of fluidized-bed biomolecule adsorption from crude feed stock requires particles with elevated density, large adsorption capacity and broad chemical stability. Moreover, combinations of small particle diameters with high densities allow for high fluidization velocities while preserving a rapid mass transfer.
This approach has been implemented by combining stable porous mineral oxide of high density (2.2, 4.7, 5.7, 9.4 g/ml) with functionalized hydrogels. The cross-linked hydrogel derivative fills the internal porosity of the beads and provides a high equilibrium binding capacity.
Various porous mineral oxides (silica, titania, zirconia and hafnia) have been characterized in term of fluidiza?tion behavior, surface reactivity and chemical resistance to harsh CIP procedures. Porous zirconia particles were also modified into ion-exchangers by suitable surface modification and intraparticle polymerization of functional?ized stable derivatives of acrylic monomers. Back-mixings in fluidized bed columns were analyzed by residence time distribution analysis of inert tracers. 328 and 218 mixing plates per meter were found for respectively, bed expansions of 1.7 and 2.9. The dynamic protein adsorption behaviors of zirconia-based polymeric anion-exchange sorbents were obtained in fluidized-bed, using BSA as model protein. A dynamic binding capacity of 62 mg/ml was observed at a fluidizing velocity of 320 cm/h. These investigations substantiate the favorable physical and chemical characteristics anticipated for dense composite beads for use as fluidized bed adsorbents.
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References
Batt B, Yabannavar V, and Singh V (1995) Expanded bed adsorption process for protein recovery from whole mammalian cell culture broth. Bioseparation 5: 41–52.
Boschetti E. (1994) Advanced sorbents for preparative protein separation purposes. J. Chromatogr. A 658: 207–236.
Chang Y, McCreath G, and Chase H (1995) Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast cell homogenates. Biotechnol. Bioeng. 48: 355–366.
Degener A, Belew M, and Velander W (1998) Zn2+ selective purification of recombinant proteins from milk of transgenic animals. J. Chromatogr. A 799 (1–2): 125–137.
Dunlap C and Carr P (1996) The effect of mobile phase on protein retention and recovery using carboxymethyl dextran-coated zirconia stationary phases. J. Liq. Chromatogr. and related technology 19 (13): 2059–2076.
Finette G, Baharin B, Mao Q, and Hearn M (1998) Optimization considerations for the purification of alpha(1)- antitrypsin using silica-based ion-exchange adsorbents in packed and expanded beds. Biotechnol. Progress 14 (2): 286–236.
Foscolo P, Gibilaro L and Waldram S (1983) A unified model for the particulate expansion of fluidized beds and flow in fixed porous media. Chem. Eng. Sci. 38 (8): 1251–1260.
Griffith C, Morris J, Robichaud M, Annen M, McCormick A, and Flickinger M (1997) Fluidization characteristics of and protein adsorption on fluoride-modified porous zirconium oxide particles. J. Chromatogr. A 776: 179–195.
Levenspiel 0 (1972) Chemical Reaction Engineering, John Wiley & Sons, New York, Chichester, Brisbane, Toronto, Singapore.
Raymond F, Rolland D, Gauthier M and Jolivet M (1998) Purification of a recombinant protein expressed in yeast: optimization of analytical and preparative chromatography. J. Chromatogr. B 706: 113–121.
Richardson J and Zaki W (1954) Sedimentation and fluidization: part I. Trans. I.stn. Chem. Engrs. 32: 35–53.
Spence C, Schaffer C, Kessler S and Bailon P (1994) Fluidized-bed receptor-affinity chromatography. Biomedical Chromatography 8: 236–241.
Thommes J, Weiher M, Karau A and Kula M (1995) Hydrodynamics and performances in fluidized bed adsorption. Biotechnol. Bioeng. 48: 367–374.
Walas S (1997) Chemical reactors. Perry’s Chemical Engineers’ Handbook, R. Perry (ed.), Mc Graw-Hill, New York.
Weaver L and Carta G (1996) Protein adsorption on cation-exchangers: comparison of macroporous and gel-composite media. Biotechnol. Progress 12 (3): 342–355.
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© 1999 Springer Science+Business Media Dordrecht
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Voute, N., Bataille, D., Girot, P., Boschetti, E. (1999). Characterization of very dense mineral oxide—gel composites for fluidized-bed adsorption of biomolecules. In: Mattiasson, B. (eds) Expanded Bed Chromatography. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1519-5_13
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DOI: https://doi.org/10.1007/978-94-017-1519-5_13
Publisher Name: Springer, Dordrecht
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