Skip to main content

Doubled haploid production in wheat through wide hybridization

  • Chapter
Doubled Haploid Production in Crop Plants


Practical breeding programs for self-pollinating crops, such as wheat (Triticum aestivum L., 2n=6x=42), must include a process of genetic fixation for uniformity of agronomic traits after genetic recombination to increase variation. Repeated selection of heterozygous materials can increase uniformity, but many generation cycles are required to reach homozygosity in loci associated with agronomic traits. Of great interest to plant breeders, haploid production followed by chromosome doubling offers the quickest method for developing homozygous breeding lines. Doubled haploids derived from heterozygous materials show complete uniformity when used as recombinant inbred lines in selection procedures. An efficient technique for producing doubled haploids thus complements conventional breeding programs. Two major methods for producing wheat haploids (polyhaploids, 2n=3x=21), one using microspores (pollen) and one using megaspores (egg cells), have been examined over last two decades. The method for producing haploids from cultured pollen has developed as an anther or pollen culture technique, and is still constrained by the different responses of wheat genotypes. On the other hand, the method using megaspores in ultra-wide crosses with Panicoides subfamilial species followed by embryo rescue has recently been developed. This paper reports on progress achieved in developing a method for wheat haploid production through ultra-wide crosses since the previous publication on producing wheat haploids through the bulbosum technique (Inagaki, 1990). The recent achievements can be attributed to the collaborative research projects of JIRCAS with the International Center for Agricultural Research in the Dry Areas (ICARDA), Syria, and with the International Maize and Wheat Improvement Center (CIMMYT), Mexico.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others


  • Barclay, I.R., 1975. High frequencies of haploid production in wheat (Triticum aestivum) by chromosome elimination. Nature 256: 410–411.

    Article  Google Scholar 

  • Inagaki, M.N., 1990. Wheat haploids through the bulbosum technique. In: Biotechnology in Agriculture and Forestry. Bajaj, Y.P.S. (Ed.) Springer-Verlag, Berlin, pp. 448–459.

    Google Scholar 

  • Inagaki, M.N., T. Nagamine and A. Mujeeb-Kazi, 1997. Use of pollen storage and detached-tiller culture in wheat polyhaploid production through wide crosses. Cereal Res.Commun. 25 (1): 7–13.

    CAS  Google Scholar 

  • Laurie, D.A. and M.D. Bennett, 1986. Wheat x maize hybridization. Can.J.Genet.Cytol. 28: 313–316.

    Google Scholar 

  • Suenaga, K. and K. Nakajima, 1989. Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays). Plant Cell Rep. 8: 263–266.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer Science+Business Media New York

About this chapter

Cite this chapter

Inagaki, M.N. (2003). Doubled haploid production in wheat through wide hybridization. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht.

Download citation

  • DOI:

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6393-9

  • Online ISBN: 978-94-017-1293-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics