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Haploid wheat isolated microspore culture protocol

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Doubled Haploid Production in Crop Plants

Abstract

Haploid production in hexaploid wheats (Triticum aestivum L.) has been achieved mainly by wide hybridization. The first wide hybridization system using Hordeum bulbusom pollen was very genotype specific. However, the subsequent use of maize, sorghum or millet pollinators has largely overcome this genotype effect and the system is being used in many programmes for research and breeding. Anther culture in hexaploid wheat has given poor frequencies and high genotype specificity. The development of a highly responsive isolated microspore culture system for barley across genotypes and using a defined induction media encouraged us to also try to improve isolated microspore cultures of wheat. The following protocol has been developed primarily on ‘Chris’, ‘Pavon 79’ and ‘Bob White’ genotypes where response is very good and it is currently being more thoroughly tested across a range of genotypes. The protocol requires co-culture of microspores with wheat ovaries and thus it is not a completely defined media. A patent application covering this protocol has been filed (Kasha and Simion, 2001).

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References

  • Comeau, A., P. Nadeau, A. Plourde, R. Simard, O. Maës, S. Kelly, L. Harper, J. Lettre, B. Landry and C.-A. St-Pierre, 1992. Media for the in ovulo culture of proembryos of wheat and wheat-derived interspecific hybrids or haploids. Plant Sci. 81: 117–125.

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  • Kasha, K.J. and E. Simion, 2001. Embryogenesis and plant regeneration from microspores. International Patent Application No. PCT/CAOO/01436 (Published electronically June 14, 2001 ).

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  • Kasha, K.J., E. Simion, R. Oro, Q. A. Yao, T. C. Hu and A. R. Carlson, 2001. An improved in vitro technique for isolated microspore culture of barley. Euphytica 120 (3): 379–385.

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© 2003 Springer Science+Business Media New York

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Kasha, K.J., Simion, E., Miner, M., Letarte, J., Hu, T.C. (2003). Haploid wheat isolated microspore culture protocol. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_13

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  • DOI: https://doi.org/10.1007/978-94-017-1293-4_13

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6393-9

  • Online ISBN: 978-94-017-1293-4

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