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Interphase Cytogenetics in Understanding Chromosome and Telomere Dynamics During Prophase I: Implications for Meiotic Telomere Movements

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Chromosomes Today

Abstract

Interphase cytogenetics (fluorescent in situ hybridization (FISH) to interphase nuclei; Cremer et al., 1986) has matured to a powerful tool capable to delineate whole chromosomes and subregions in nuclei, which usually lack chromosome-specific landmarks accessible to conventional staining methods. The possibility to simultaneously label several targets in interphase nuclei has led to widespread use of this technique in diagnostic settings and investigations on three-dimensional (3D) genome and chromosome organization during the cell cycle. Somatic interphase nuclei undergo architectural changes during development and differentiation. The most striking alterations of chromosome architecture and distribution occur during the extended prophase of the first of the two meiotic divisions. This article will focus on the contribution of interphase cytogenetics to the current view of chromosome organization and telomere dynamics during first meiotic prophase. It will be discussed that mammalian KU70 dsDNA end-binding protein may not play a structural role at the meiotic telomere. I will discuss a putative pathway linking the meiotic telomere to cell cycle progression, cytoskeleton and nuclear envelope that is based on recently disclosed two-hybrid interactions of the meiosis-specific telomere protein Ndj 1p of budding yeast. Other aspects of meiotic chromosome biology are dealt with elsewhere (e.g., Loidl, 1990; Kleckner, 1996; Roeder, 1997; Moens et al., 1998; Smith and Nicolas, 1998; Zickler and Kleckner, 1998; Villeneuve and Hillers, 2001; Cowan et al., 2001).

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  1. Max-Planck-Inst. für Molekulare Genetik, Ihnestr. 73, D-14195, Berlin, Germany

    Harry Scherthan

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Scherthan, H. (2004). Interphase Cytogenetics in Understanding Chromosome and Telomere Dynamics During Prophase I: Implications for Meiotic Telomere Movements. In: Schmid, M., Nanda, I. (eds) Chromosomes Today. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1033-6_13

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  • DOI: https://doi.org/10.1007/978-94-017-1033-6_13

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