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Immortalisation of Primary Cells

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Abstract

In nature the progression of cells through the cell cycle leading to proliferation of is normally subject to tight regulation by means of the complex and coordinated interaction between cyclins (A-E) and cyclin dependent kinases (for a recent review see Abu-Absi, 2000). ‘Immortalised’ cells escape the normal controls of the cell cycle. Thus, they divide and grow continuously beyond the limits seen in “normal” tissues and primary cells. Historically these continuous cell lines (CCLs) have been primarily isolated from tumours and embryos. In the case of tumours the resulting cell lines may arise from cancer cells that undergo secondary mutations which stabilise or otherwise modify the expression of oncogenes. Viral sequences may also be responsible for the appearance of immortalised cells from cultures of primary cells. Nevertheless, in general where cell lines appear to arise spontaneously from such cultures the mechanism of their apparent replicative immortality is unknown.

Keywords

Primary Cell Genetically Modify Organism Continuous Cell Line Rapid Cell Proliferation Animal Cell Technology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  1. 1.NIBSCSouth Mimms, HertsUK
  2. 2.University of PaisleyGlasgowScotland, UK

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