Abstract
Rat tumor cell line U41 is derived from rat malignant fibrous histiocytoma (MFHC) developed in the part of subcutaneous implantation of polyetherurethane (PEU) films. U41 cells is suppressed in gap junctional intercellular communication (GJIC). Western blot and RT-PCR analysis indicate that the expression levels of the gap junction protein connexin43 (Cx43) are decreased in this cell line. Rat Cx43 cDNA was transfected into U41 derived subclone U41-22. A stable Cx43 transfectant clone, which acquired high levels of Cx43 expression and the capacity of GJIC, was established and compared with mock transfectant control clone. Cx43 transfected clone U41-22/Cx43 showed reduced growth rate, recovery of contact inhibition and loss of colony formation ability in soft agar. These results strongly suggests that suppression of Cx43 expression play an important role in development of rat MFHC caused by PEU implantation, and Cx43 acts as tumor suppressor to PEU induced tumor.
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© 2002 Springer Science+Business Media Dordrecht
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Ichikawa, A., Tsuchiya, T. (2002). Reversion of Transformed Phenotype of Polyetherurethane-Induced Tumor Cells by Cx43 Transfection. In: Shirahata, S., Teruya, K., Katakura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0728-2_48
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DOI: https://doi.org/10.1007/978-94-017-0728-2_48
Publisher Name: Springer, Dordrecht
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