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Improvement of Sialylation in Chinese Hamster Ovary (CHO) Cell Culture by Feeding N-Acetylmannosamine

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Book cover Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 12))

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Abstract

The degree of sialylation is a major issue in the development of therapeutic glycoproteins. In this study, the feeding of N-acetylmannosamine (ManNAc) to Chinese hamster ovary (CHO) cell cultures was investigated as a method of improving sialylation profile, using a model glycoprotein we call, “Glycoprotein X”. For the purpose, moreover, a simplified 4-step method for sialylation profile analysis was developed without the use of special or expensive equipment. The addition of ManNAc into the culture medium largely improved sialylation of Glycoprotein X in a dose-dependent manner up to 20 mM ManNAc.

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Authors and Affiliations

  1. Irvine Scientific, Santa Ana, CA, 92705, USA

    Yoichi Oba, Tom Fletcher, Mark Hisaka & William Jay Treat

Authors
  1. Yoichi Oba
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  2. Tom Fletcher
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  3. Mark Hisaka
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  4. William Jay Treat
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Editor information

Editors and Affiliations

  1. Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan

    Sanetaka Shirahata , Kiichiro Teruya  & Yoshinori Katakura ,  & 

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© 2002 Springer Science+Business Media Dordrecht

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Oba, Y., Fletcher, T., Hisaka, M., Treat, W.J. (2002). Improvement of Sialylation in Chinese Hamster Ovary (CHO) Cell Culture by Feeding N-Acetylmannosamine. In: Shirahata, S., Teruya, K., Katakura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0728-2_37

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  • DOI: https://doi.org/10.1007/978-94-017-0728-2_37

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5934-5

  • Online ISBN: 978-94-017-0728-2

  • eBook Packages: Springer Book Archive

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