Abstract
Recombinant CHO and NS0 cells were grown in a stirred laboratory bioreactor equipped with an integral tubular dialysis membrane with either 12 or 100 kDa molecular weight cut-off. The use of the dialysis membrane, which allowed perfusion of nutrients through its pores, enabled considerable increases in cell and product concentrations over those obtained in batch culture, with substantial improvements in growth and product yields on serum.
Keywords
- Mass Transfer Coefficient
- Dialysis Membrane
- Dissolve Oxygen Tension
- High Molecular Weight Fraction
- Viable Cell Concentration
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References
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© 2002 Springer Science+Business Media Dordrecht
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Yamaji, H., Amos, B., Emery, A.N., Al-Rubeai, M. (2002). Dialysis Perfusion Culture of Animal Cells for the Production of Biologicals. In: Shirahata, S., Teruya, K., Katakura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0728-2_21
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DOI: https://doi.org/10.1007/978-94-017-0728-2_21
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5934-5
Online ISBN: 978-94-017-0728-2
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