Abstract
Microcarrier (M.C.) for the large-scale culture of anchorage dependent animal cells is available. We have established a microcarrier perfusion culture (1L-scle) of recombinant CHO cells to produce humanized monoclonal antibody, KM8400 and evaluated the productivity of this culture. A cell density of 25×107 cells/ml was reached on day 13 and this culture maintained for 26 days. The total amount of KM8400 produced was 35g.
During the culture’s stationary phase, specific production rate was 13 fold higher than in the growth phase. Surprisingly, we found that the productivity of this cell line increased with the increase of G1 phase cell population.
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© 2002 Springer Science+Business Media Dordrecht
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Makimoto, Y., Takahashi, E., Takasugi, H. (2002). Relationship Between Cell Cycle Phases and Monoclonal Antibody Production in Microcarrier Perfusion Culture of Recombinant CHO Cells. In: Shirahata, S., Teruya, K., Katakura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0728-2_19
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DOI: https://doi.org/10.1007/978-94-017-0728-2_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5934-5
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