Abstract
Five clones of mouse-human hybridomas secreting antibodies to peanut allergens were generated from peripheral blood lymphocytes transformed with Epstein-Barr virus followed by cell fusion with mouse myeloma cells. Western blot analysis showed that two antibodies reacted to Ara h1 peanut allergen. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed two antibody-binding sequences in Ara h1 protein.
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© 2003 Springer Science+Business Media Dordrecht
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Shinmoto, H., Takahashi, T., Yamagishi, K., Kimura, T., Suzuki, M., Maleki, S.J. (2003). Generation of Mouse-Human Hybridomas Secreting Antibodies Against Peanut Allergens. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_46
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DOI: https://doi.org/10.1007/978-94-017-0726-8_46
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6557-5
Online ISBN: 978-94-017-0726-8
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