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Generation of Mouse-Human Hybridomas Secreting Antibodies Against Peanut Allergens

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 13))

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Abstract

Five clones of mouse-human hybridomas secreting antibodies to peanut allergens were generated from peripheral blood lymphocytes transformed with Epstein-Barr virus followed by cell fusion with mouse myeloma cells. Western blot analysis showed that two antibodies reacted to Ara h1 peanut allergen. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed two antibody-binding sequences in Ara h1 protein.

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References

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Author information

Authors and Affiliations

  1. National Food Research Institute, 2-1-12 Kannondai, Tsukuba, 305-8642, Japan

    Hiroshi Shinmoto

  2. National Agricultural Research Center for Tohoku Region, 50 Harajuku-Minami, Arai, Fukushima, 960-2156, Japan

    Toshihiro Takahashi, Kenji Yamagishi, Toshiyuki Kimura & Masahiro Suzuki

  3. Southern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 1100 Robert E. Lee Boulevard, 70124, New Orleans, Louisiana, USA

    Soheila J. Maleki

Authors
  1. Hiroshi Shinmoto
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  2. Toshihiro Takahashi
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  3. Kenji Yamagishi
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  4. Toshiyuki Kimura
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  5. Masahiro Suzuki
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  6. Soheila J. Maleki
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Editor information

Editors and Affiliations

  1. Department of Applied Biological Science, Tokyo Noko University, Tokyo, Japan

    Kazumi Yagasaki , Yutaka Miura , Makoto Hatori  & Yoshihiro Nomura , ,  & 

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© 2003 Springer Science+Business Media Dordrecht

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Shinmoto, H., Takahashi, T., Yamagishi, K., Kimura, T., Suzuki, M., Maleki, S.J. (2003). Generation of Mouse-Human Hybridomas Secreting Antibodies Against Peanut Allergens. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_46

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  • DOI: https://doi.org/10.1007/978-94-017-0726-8_46

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6557-5

  • Online ISBN: 978-94-017-0726-8

  • eBook Packages: Springer Book Archive

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