Abstract
We showed here that the scale-up of the production of the rickettsiae could benefit of the control of the migration properties of the host cells. Indeed, bead to bead transfer of endothelial cells, either normal or SV40 transformed and immortalized, was shown to be efficient in cell subculturing using Cytodex 3 microcarriers, avoiding therefore often fastidious, time- and labour-consuming procedures and possibly also physiological damage to the cells.
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© 2003 Springer Science+Business Media Dordrecht
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Hendrick, V. et al. (2003). Endothelial Cells Culture and Its Use to Produce a Vaccine Against Rickettsiae. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_40
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DOI: https://doi.org/10.1007/978-94-017-0726-8_40
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6557-5
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