Abstract
Hyperosmotic pressure has been recognized as being an economical solution to increase q in hybridoma (1,4), transfectoma (2), and rCHO cell cultures (5). Despite the potential of commercial strategies based on hyperosmotic pressure, the use of hyperosmolar medium has not been popular because cell growth is depressed at elevated osmolality. (1,4). Among various strategies to overcome depressed cell growth at elevated osmolality, the use of osmoprotective compounds such as glycine betaine is probably most feasible for improved foreign protein production (3,5). However, the efficacy of the simultaneous use of hyperosmotic pressure and glycine betaine was found to be variable among rCHO cell lines (5). In this study, we investigated the feasibility of simple biphasic culture strategy based on hyperosmotic culture for improved foreign protein production from rCHO cells. In the biphasic culture, the rCHO cells producing humanized antibody are first cultivated in the standard medium for cell growth and then in the hyperosmolar medium for antibody production.
Keywords
- Glycine Betaine
- rCHO Cell
- Recombinant Chinese Hamster Ovary Cell
- Increase Cell Concentration
- Chinese Hamster Ovary Cell Culture
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
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© 2003 Springer Science+Business Media Dordrecht
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Kim, M.S., Kim, N.S., Sung, Y.H., Lee, G.M. (2003). Biphasic Culture Strategy Based on Hyperosmotic Pressure for Improved Humanized Antibody Production in Chinese Hamster Ovary Cell Culture. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_27
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DOI: https://doi.org/10.1007/978-94-017-0726-8_27
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6557-5
Online ISBN: 978-94-017-0726-8
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