Skip to main content
SpringerLink
Log in

Development of a Serum-Free Medium Supplemented with Hydrolysate for the Suspension Culture of Recombinant CHO Cells Producing Human Thrombopoietin

  • Conference paper
Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 13))

  • 422 Accesses

Abstract

Human thrombopoietin (hTPO) is a potential therapeutic glycoprotein for the amelioration of thrombocytopenia associated with chemotherapy, irradiation and bone marrow transplantation [1]. The hTPO has been expressed in Chinese hamster ovary (CHO) cells, but its expression level is low (0.7 – 8.5 μg/mL) [2]. To increase the hTPO expression level, culture medium specifically designed for hTPO production needs to be developed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ulich, T.R., del Castillo, J., Yin, S., Swift, S., Padilla, D., Senaldi, G, Bennett, L., Shutter, J., Bogenberger, J., Sun, D., Samal, B., Shimamoto, G., Lee, R., Steinbrink, R., Boone, T., Sheridan, W.T. and Hunt, P. (1995) Megakaryocyte growth and development factor ameliorates carboplatin-induced thrombocytopenia in mice, Blood 86, 971 - 976.

    PubMed  CAS  Google Scholar 

  2. Kim, T.K., Ryu, J.S., Chung, J.Y., Kim, M.S. and Lee, GM. (2000) Osmoprotective effect of glycine betaine on thrombopoietin production in hyperosmotic Chinese hamster ovary cell culture: Clonal variations, Biotechnol. Prog. 16, 775 - 781.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-Dong, Yusong-Gu, Daejon, 305-701, Korea

    Yun Hee Sung & Gyun Min Lee

Authors
  1. Yun Hee Sung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gyun Min Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Applied Biological Science, Tokyo Noko University, Tokyo, Japan

    Kazumi Yagasaki , Yutaka Miura , Makoto Hatori  & Yoshihiro Nomura , ,  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer Science+Business Media Dordrecht

About this paper

Cite this paper

Sung, Y.H., Lee, G.M. (2003). Development of a Serum-Free Medium Supplemented with Hydrolysate for the Suspension Culture of Recombinant CHO Cells Producing Human Thrombopoietin. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_21

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-0726-8_21

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6557-5

  • Online ISBN: 978-94-017-0726-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation