Skip to main content

Grape pulp, grape pips and their mixture: Novel substrates or supports for solid state fermentation

Pulpe de raisin, pépins de raisins et leur mélange: Nouveaux substrats ou supports pour la fermentation en milieu solide

  • Chapter
Advances in Solid State Fermentation

Summary

Grape marc, comprising of grape pulp and grape pips, is generated in high quantities during ethanol production, but has no significant commercial value. The components of the grape marc, individually or in combination, can serve as substrate or support in solid state fermentation. The potential benefits of sieved fractions (800-3150 µm particle size) was, therefore, evaluated using Trichoderma viride, with or whithout enrichment. Based on sorption isotherm profiles, grape pulp showed the best performance, though its ability to promote biomass formation was the lowest. The latter could be improved by enrichment with glucose. It, thus, offers potential as a reusable support. Grape pips promoted high biomass, without any enrichment, but was low in water retention capacity. The performance of the mixture of grape pulp + grape pips (1:1, w/w) was in between that of the individual fractions. Data also indicated that the mycelial fragments frozen at -20°C up to 15 days can be used as an inoculum.

Resume

Le marc de raisin, dont sont issus la pulpe et les pépins, est un sous-produit de la vinification largement disponible en sortie de distillerie, mais sans grande valeur commerciale. Les constituants du marc, individuellement ou en mélange, peuvent être utilisés comme substrats ou supports en fermentation en milieu solide. Ainsi, en présence de Trichoderma viride, les aptitudes de fractions solides tamisées, aux dimensions comprises entre 800 et 3150 µm, ont été comparées avec et sans enrichissement. Sur la base des isothermes de sorption, la pulpe de raisin montre la plus grande rétention d’eau, bien que son aptitude à favoriser la formation de biomasse soit la plus faible. Cette dernière peut être améliorée par enrichissement en glucose. Ainsi, la pulpe se présente potentiellement comme un support réutilisable. Les pépins de raisins favorisent les plus fortes productions de biomasse, même sans enrichissement, mais ils ont les plus faibles capacités de rétention d’eau. Les performances du mélange pulpe + pépins (1:1, m/m) correspondent aux valeurs moyennes des fractions individuelles. Les résultats indiquent aussi que le mycélium conservé congelé à -20°C durant moins de 15 jours peut être utilisé comme inoculum.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Cochet, N., Tyagi, R., Ghose, T. and Lebeault, J.M. 1984. ATP measurement for cellulase production control. Biotechnol. Lett., 6: 9 – 16.

    Article  Google Scholar 

  • Debeaufort, F. 1994. Etude des transferts de matière au travers de films d’emballages. Thèse de Doctorat. Université de Bourgogne, France.

    Google Scholar 

  • Delas, J. 1967. Utilisation des marcs de raisin comme amendement organique dans les sols de vignoble. Vignes et Vins, 164: 19 – 29.

    Google Scholar 

  • Faure, D. and Deschamps, A.M. 1990. Physicochemical and microbiological aspects in composting of grape pulps. Biol.Wastes 34: 251 – 258.

    Article  CAS  Google Scholar 

  • Hassid, W.Z. and Neufeld, E.F. 1964. Determination of starch in plant tissues. In R.L. Whistler (Ed.), Methods in Carbohydrate Chemistry-4. Acad. Press, New York, pp 33 – 36.

    Google Scholar 

  • Hysert, D.W., Kovecses, F. and Morisson, N.M. 1976. A firefly bioluminescense ATP assay method for rapid detection and enumeration of brewery microorganisms. J. Am. Soc. Biochem. Chem., 34: 145 – 150.

    CAS  Google Scholar 

  • ITEB. 1988. Les sous-produits en alimentation animale. Guide de l’utilisation. Institut Technique d’Elevage Bovin. Technipel, Paris.

    Google Scholar 

  • Lonvaud-Funel, A. and Joyeux, A. 1982. Application de la bioluminescence au dénombrement des microorganismes vivants dans le vin. Connaissance Vigne Vin, 46: 1224 – 1226.

    Google Scholar 

  • Lossin, R.D. 1970. Compost studies: degree of decomposition. Compost Sci., 11, 17 – 27.

    Google Scholar 

  • Miller, G.L. 1959. Use of dinitrosalicilic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426 – 429.

    Article  CAS  Google Scholar 

  • Mustin, M. 1987. Le compost: gestion de la matière organique. In F. Dubusc (Ed.), Librairie Cocagne, Paris.

    Google Scholar 

  • Pirt, S.J. 1967. A kinetic study of the mode of growth of surface colonies of bacteria and fungi. J. Gen. Microbiol. 47: 181 – 187.

    Article  CAS  Google Scholar 

  • Serrano-Carreon, L., Hathout, Y., Bensoussan, M. and Belin, J.M. 1992. Lipid accumulation in Trichoderma species. FEMS Microbiol. Leu., 93: 181 – 188.

    Article  CAS  Google Scholar 

  • Serrano-Carreon, L., Hathout, Y., Bensoussan, M. and Belin, J.M. 1993. Metabolism of linoleic acid or mevalonate and 6-pentyl-a-pyrone biosynthesis by Trichoderma species. Appl. Environ. Microbiol. 59: 2945 – 2950.

    CAS  Google Scholar 

  • Suberkropp, K., Gessner, M.O. and Chauvet, E. 1993. Comparison of ATP and ergosterol as indicators of fungal biomass associated with decomposing leaves in streams. Appl. Environ. Microbiol. 59: 3367 – 3372.

    CAS  Google Scholar 

  • Thierry, A. and Chicheportiche, R. 1988. Use of ATP bioluminescence measurement for the estimation of biomass during biological humification. Appl. Microbiol. Biotechnol. 28: 199 – 202.

    Article  CAS  Google Scholar 

  • Trinci, A.P.J. 1971. Influence of the width of the peripherical growth zone on the radial rate of fungal colonies on solid media. J. Gen. Microbiol. 67: 325 – 344.

    Article  Google Scholar 

  • Vaccarino, C., Lo Curto, R.B., Tripodo, M.M., Patane, R. and Ragno, A. 1992. Grape marc as a source of feedstuff after chamical treatments and fermentation with fungi. Bioresource Tech. 40: 35 – 41.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Bensoussan, M., Serrano-Carreon, L. (1997). Grape pulp, grape pips and their mixture: Novel substrates or supports for solid state fermentation. In: Roussos, S., Lonsane, B.K., Raimbault, M., Viniegra-Gonzalez, G. (eds) Advances in Solid State Fermentation. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0661-2_18

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-0661-2_18

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4904-9

  • Online ISBN: 978-94-017-0661-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics