Abstract
A high cell density process for C. utilis production on molasses was developed. A demonstration plant producing 70.8 kg h−1 was operated in 1990 and 1991. C. utilis was continuously cultivated at cell densities of 80 to 110 kg m−3 in an especial 10.5 m3 jet loop fermentor having an oxygen transfer rate of 350 mol O2m−3 h−1 at 1.1 VVM. The yeast was concentrated by a rotary filter yielding a 24% solids yeast cake, which is continuously thermolysed. The thermolysed product-which can be used as a natural flavor enhancer for food and fed — had a protein digestibility higher than 90%. The technology requires 20% less in capital investment and consumes only one third of the water involved in fermentation, if compared with conventional yeast production processes, being therefore a highly competitive alternative.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
Literature Cited
Guidoboni, G.E., The problems of large-scale yeast production, In: G.G. Stewart, I. Russell, R.D. Klein and R.R. Hiebsch (Eds.), Biological Research on Industrial Yeast vol. 1, p. 47 CRC Press, Boca Raton, Florida (1987).
Westalake, R., Chem. Ing. Tech. (Germany) 58, 934 (1986).
Staff article., Food Technology (USA) 43 7, 50 (1989).
Blenke, H., Biochemical loop reactors, In: H.J. Rehm and G. Reed (Eds.), Biotechnology vol. 2, p. 465 VCH Verlagsgesellschaft, Weinheim (1985).
Flores, Z., L.B. Flores, S. Garcia, J. Corona, O. Melchy and M. de la Torre, High cell density computer control of Candida utilis yeast production on molasses. In: N. Karim and G. Stephanopoulos (Eds.), Modeling and Control of Biotechnical Processes and Computer Applications in Fermentation Technology, p. 227 Pergamon Press, New York (1992).
Wang, H.Y., C.L. Cooney and D.I.C. Wang, Biotechnol. Bioeng., 21, 975 (1979).
Gonzalez, A. and E. Chong, Producción de levadura en el reactor de 10 m’ con control por computadora. Report No. PTE-054. Department of Biotechnology, CINVESTAV, Mexico (1990).
Hurrel, R.F., P. Lerman and K.J. Carpenter, J. Food Science, 44, 1221 (1979).
Flores Cotera, L.B. and S. Garcia Salas, U.S. patent appl. serial number 07/963,980 to CINVESTAV, (October, 1992 ).
Vasey, K.B. and K.A. Powell, Biotechnology and Genetic Eng. Reviews, 2, 285 (1984).
Katinger, W.D.H., European J. Appl. Microbiol, 3, 103 (1976).
Gutiérrez, E., Estudio técnicoeconómico sobre la separación de levadura forrajera por centrifugacién, floculación, sedimentación y filtración. II Elección del arreglo óptimo. Report No. PTB-001. Department of Biotechnology, CINVESTAV, México. (1987).
Gutiérrez, E., Termólisis de la levadura C. Utilis. Report No. PTE-019. Department of Biotechnology, CINVESTAV, México. (1987).
De la Torre, M. and M. Luna, Termólisis de la levadura NRRL-Y 900. Report No. PTE-046. Department of Biotechnology, CINVESTAV, México. (1989).
De la Torre, M. and M. Luna, Evaluación de los procesos de autólisis y termólisis en cuanto al incremento en digestibilidad proteica y el efecto en el contenido de lisina de levadura tórula, tanto a nivel laboratorio como en planta piloto. Report No. PTE-058. Department of Biotechnology, CINVESTAV, México. (1991).
Estévez, R., Levadura forrajera a partir de las mieles finales de cana, In: ICIDCA (Ed.), Los derivados de la cana de azúcar p. 288 Editorial Científico-Técnica, La Habana, Cuba (1980).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
de la Torre, M., Flores, L.B., Chong, E. (1994). High Cell Density Yeast Production: Process Synthesis and Scale-Up. In: Galindo, E., Ramírez, O.T. (eds) Advances in Bioprocess Engineering. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0641-4_9
Download citation
DOI: https://doi.org/10.1007/978-94-017-0641-4_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-4459-4
Online ISBN: 978-94-017-0641-4
eBook Packages: Springer Book Archive