Abstract
Characterization of T-cell clones and identification of functional subsets of the helper T-cells with polarized cytokine production is based on testing of cytokine expression. Several methods have been developed that allow cytokine expression to be measured like ELISA, RT-PCR, ELISPOT, ISH and flowcytometry. Among all these methods, monitoring of cytokine production using flowcytometric analysis has its own advantages and disadvantages. Multi-parametric characterization of cytokine production on single cell basis, without long-term culture and cloning along with high throughput of samples is main feature attached to flowcytometric analysis. The interpretation may be difficult at times due to change in the phenotype of the cells. Cells with similar surface phenotype but synthesizing different cytokines and having different functional characteristics can be analyzed with this technique.
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© 2003 Springer Science+Business Media Dordrecht
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Arora, S.K. (2003). Analysis of intracellular cytokines using flowcytometry. In: Sobti, R.C., Krishan, A. (eds) Advanced Flow Cytometry: Applications in Biological Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0623-0_5
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DOI: https://doi.org/10.1007/978-94-017-0623-0_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6368-7
Online ISBN: 978-94-017-0623-0
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